Abstract
The aims of this chapter are to learn to distinguish rhizobia from other microorganisms by cell morphology, staining reactions, and growth responses on various media, and to show how media for rhizobia can be modified. Root nodule bacteroids, cultures of rhizobia and bradyrhizobia, and other microorganisms are examined under the microscope using staining techniques. They are also cultured on indicator media. The growth reactions of five strains of rhizobia and bradyrhizobia are observed on media containing combinations of C and N from different sources.
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Key References
Allen, O.N., and E.D. Allen. 1950. Biochemical and symbiotic properties of the rhizobia. Bacteriol. Rev. 14: 273–330.
Fred, E.B., I.L. Baldwin, and E. McCoy. 1932. Root Nodule Bacteria and Leguminous Plants. University of Wisconsin, Madison.
Graham, P.H., and C.A. Parker. 1964. Diagnostic features in the characterization of the root nodule bacterial of legumes. Plant Soil 20: 383–396.
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© 1994 Springer-Verlag New York, Inc.
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Somasegaran, P., Hoben, H.J. (1994). Cultural Properties, Cell Morphology, and Nutritional Requirements of Rhizobia. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_3
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DOI: https://doi.org/10.1007/978-1-4613-8375-8_3
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-8377-2
Online ISBN: 978-1-4613-8375-8
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