Producing Broth Cultures in Simple Glass Fermentors

  • Padma Somasegaran
  • Heinz J. Hoben

Abstract

A small glass fermentor of 2–3-liter capacity can be used to produce broth cultures to be applied as liquid inoculants in field experiments. It is also suitable for producing starter cultures for medium-sized commercial fermentors. In this chapter, a glass fermentor is assembled and then used for small-scale production of broth cultures. The broth cultures are monitored periodically for quality control, including checks for contamination and the progress of growth and cell multiplication.

Keywords

Burner Hydrogen Peroxide Agar Manifold Rubber 

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Key Reference

  1. Thompson, J A. 1980. Production and quality control of legume inoculants. pp. 489–533. In F.J. Bergersen (ed.) Methods for Evaluating Biological Nitrogen Fixation. John Wiley & Sons, New York.Google Scholar

Copyright information

© Springer-Verlag New York, Inc. 1994

Authors and Affiliations

  • Padma Somasegaran
    • 1
  • Heinz J. Hoben
    • 1
  1. 1.University of Hawaii NifTAL ProjectPaiaUSA

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