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Observing the Infection Process

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Handbook for Rhizobia

Abstract

Clover (Trifolium spp.) rhizobia enter their host’s roots through the root hairs. Infection is preceded by a deformation of root hairs and the forming of an infection thread that can be observed directly under the microscope. Root hair deformations may also be caused by nonnodulating strains of Rhizobium. Nonnodulating strains used in this chapter cause no infection threads to form. The purpose of this chapter is to observe the infection of legume roots by rhizobia. Clover seedlings are inoculated with rhizobia and stages of the infection process are then observed under the microscope.

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Key References

  • Dart, P.J. 1974. The infection process, pp. 381–429. In A. Quispel (ed.) The Biology of Nitrogen Fixation. North Holland Publishing Company, Amsterdam, Holland.

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  • Fáhraeus, A. 1957. The infection of clover root hairs by Nodule bacteria studied by a simple glass slide technique. J. Gen. Microbiol 16: 374–381.

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  • Hubbell, D.H. 1981. Legume infection by Rhizobium: A conceptual approach. Bioscience 31: 832–837.

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  • Yao, P.Y., and J.M. Vincent. 1969. Host specificity in the root hair “curling factor” of Rhizobium spp. Aust. J. Biol. Sci. 22: 413–423.

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© 1994 Springer-Verlag New York, Inc.

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Somasegaran, P., Hoben, H.J. (1994). Observing the Infection Process. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_2

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  • DOI: https://doi.org/10.1007/978-1-4613-8375-8_2

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4613-8377-2

  • Online ISBN: 978-1-4613-8375-8

  • eBook Packages: Springer Book Archive

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