Abstract
In this experiment, two serologically distinct strains of Bradyrhizobium japonicum are mixed in equal numbers to obtain mixed-strain inocula for studying interstrain competition for nodulation on soybean (Glycine max). The immunodiffusion technique, which is commonly employed for the detection of soluble antigens, is adopted for determining the identities of the strains occupying the nodules. The soluble somatic antigen characteristics of a particular strain and its bacteroid form are demonstrated as useful markers in identifying strains in competition experiments.
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Key References
Dudman, W.F., and J. Brockwell. 1965. Ecological studies of root-nodule bacteria introduced into field environments. I. A survey of field performance of clover inoculants by gel immune diffusion serology. Aust. J. Agric. Res. 19: 739–747.
Skrdleta, V. 1969. Application of immunoprecipitation in agar gel for the serological typing of soybean root-nodules. Folia Microbiologica (Praha) 14: 32–345.
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© 1994 Springer-Verlag New York, Inc.
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Somasegaran, P., Hoben, H.J. (1994). Determining Strain Occupancy in Soybean Nodules by Gel Immunodiffusion. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_12
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DOI: https://doi.org/10.1007/978-1-4613-8375-8_12
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-8377-2
Online ISBN: 978-1-4613-8375-8
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