Abstract
Immunodiffusion in gel allows for the recognition of antigenically identical strains and for differentiating between closely related nonidentical strains. In this chapter, soluble and diffusible heat-stable somatic antigens of varying molecular sizes are studied. The antigen and test antiserum are filled in separate wells and allowed to diffuse toward each other slowly through agar, which provides the support medium. Line(s) of precipitation would form where optimal proportions of antigen and antibody molecules meet. Immunodiffusion is useful in analyzing the number of soluble somatic antigens present on the cells of rhizobia.
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Key References
Dudman, W.F. 1964. Immune diffusion analysis of the extracellular soluble antigens of two strains of Rhizobium meliloti. J. Bacteriol. 88: 782–794.
Dudman, W.F. 1971. Antigenic analysis of Rhizobium japonicum by immunodiffusion. Appl. Microbiol. 21: 973–985.
Vincent, J.M. 1970. A Manual for the Practical Study of Root Nodule Bacteria. IBP Handbook no. 15. Blackwell Scientific Publications, Oxford.
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© 1994 Springer-Verlag New York, Inc.
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Somasegaran, P., Hoben, H.J. (1994). Performing Rhizobial Antigen-Antibody Reactions by Gel Immunodiffusion. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_11
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DOI: https://doi.org/10.1007/978-1-4613-8375-8_11
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-8377-2
Online ISBN: 978-1-4613-8375-8
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