Abstract
This experiment is designed to demonstrate that the Bradyrhizobium japonicum bacteroids in soybean (Glycine max) nodules share common antigenic properties with its bacterial genotype in culture. Therefore, bacteroids from fresh, desiccated, or oven-dried nodules can be used directly for identifying the occupant strain by simple agglutination. This direct method eliminates the time-consuming steps of isolating the strain in pure culture prior to its use as an antigen in an agglutination reaction.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Key References
Means, U.M., H.W. Johnson, and R.A. Date. 1964. Quick serological method of classifying strains of Rhizobium japonicum in nodules. J. Bacteriol. 87: 547–533.
Somasegaran, P., R. Woolfenden, and J. Halliday. 1983. Suitability of oven-dried root nodules for Rhizobium strain identification by immunofluorescence and agglutination. J. Appl. Bacteriol. 55: 253–261.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer-Verlag New York, Inc.
About this chapter
Cite this chapter
Somasegaran, P., Hoben, H.J. (1994). Agglutinating Antigens from Root Nodules. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_10
Download citation
DOI: https://doi.org/10.1007/978-1-4613-8375-8_10
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-8377-2
Online ISBN: 978-1-4613-8375-8
eBook Packages: Springer Book Archive