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Confocal Microscopy of Botanical Specimens

  • Chapter
Multidimensional Microscopy

Abstract

In general, plant cells are highly heterogeneous with reference to their optical properties, i.e.,absorbance, refractive index, fluorescence, phosphorescence, and birefringence. Cellular organelles and extracellular structures such as light absorbing chloroplasts and pigments, the cuticular layer and waxes found on the surface of epidermal cells, the cell wall, the exine of the pollen grains, and starch, lipid, and protein granules commonly found in the storage tissues all differ significantly from each other and the surrounding aqueous medium in terms of optical properties. This optical heterogeneity creates a major problem in confocal microscopy of plant cells.

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References

  • Acharya, R. S., Cheng, P. C., Samarabandu, J. K., Summers, R. G., Chen, L. H. and Musi al, C. (1990): Multi-dimensional image analysis of confocal images, Trans. Roy. Micro., 1:289–292.

    Google Scholar 

  • Agard, D. A. and Sedat, J. W. (1983): Three-dimensional architecture of a polytene nucleus, Nature, 302: 676–681.

    Article  PubMed  CAS  Google Scholar 

  • Agard, D. A., Hiraoka, Y., Shaw, P. and Sedat, J. W. (1989): Fluorescence microscopy in three dimensions, Meth. Cell Biol., 30: 353–377.

    Article  CAS  Google Scholar 

  • Baker, E. A. (1982): Chemistry and morphology of plant epicuticular waxes, in: The Plant Cuticle, eds. Cutler, D. F., K. L. Alvin and C. E. Price, Academic Press, London, 139–165.

    Google Scholar 

  • Bommineni, V. R. and Cheng, P. C. (1990): The use of confocal microscopy to study the developmental morphology of shoot apical meristems: a procedure to prepare the specimen, Maize Genet. Coop. Newsletter, 64: 34.

    Google Scholar 

  • Bommineni, V. R., Cheng, P. C., Samarabandu, J. K., Lin, T. H. and Walden, D. B. (1993): Estimation of cell number in the maize apical meri stematic dome and a three dimensional view of the reconstructed apical meristem by confocal microscopy and multidimensional image analysis, Scanning, 15, Sup. III, 21–22.

    Google Scholar 

  • Bown, M. and Wolf, E. (1980): Principles of Optics, Pergamon Press, Oxford.

    Google Scholar 

  • Boyde, A. (1990): Direct recording of stereoscopic pair from disk-scanning confocal light microscopes, in: The Handbook of Biological Confocal Microscopy,Pawley, J.,Plenum Press, New York, 163–168.

    Google Scholar 

  • Buican, T. N. (1990): Real-time Fourier transform spectrometry for fluorescence imaging and flow cytometry, Spie,1205: 126–133.

    Article  Google Scholar 

  • Burke, M., and Clapham, D. (1993): Simultaneous ultraviolet and visible excitation confocal microscopy, in this volume.

    Google Scholar 

  • Chen, V. (1990): Non-laser illumination for confocal microscopy, in: The Handbook of Biological Confocal Microscopy. ed. Pawley, J., Plenum Press, New York, 69–76.

    Google Scholar 

  • Cheng, P. C., Walden, D. B. and Greyson, R. I. (1979): Improved plant microtechnique for TEM, SEM and LM specimen preparation, Natl. Sci. Counc. Month. Rep. China, 7: 1000–1007.

    Google Scholar 

  • Cheng, P. C., Greyson, R. I., and Walden, D. B. (1986): Anther cuticle of Zea mays, Can. J. Bot., 64: 2088–2079.

    Article  Google Scholar 

  • Cheng, P. C. and Kim, H. G. (1989): The use of x-ray contact microradiography in the study of silica deposition in the leaf blade, Maize Genet. Coop. News Letter, 63: 45–47.

    Google Scholar 

  • Cheng, P. C., Newberry, S. P., Kim, H. and Hwang, I. S. (1990): X-ray microradiography and shadow projection x-ray microscopy, in: Modern Microscopy, eds. Duke, P. J. and Michette, A. G., Plenum Press, New York.

    Google Scholar 

  • Cheng, P. C. and Summers, R. G. (1990): Image contrast in confocal light microscopy. In: The Handbook of Biological Confocal Microscopy, ed. Pawley, J., Plenum Press, New York. 179–195.

    Google Scholar 

  • Cheng, P. C. and Pareddy, D. (1990): Confocal light microscopy: A new tool for maize research. Maize Genet. Coop. Newsletter, 64, 32–34.

    Google Scholar 

  • Cheng, P. C., Kim, H. and Lin, T. H. (1992): The study of silica deposition in the leaf blade of Zea mays L. by x-ray contact microradiography and confocal microscopy, in: X-ray microscopy III, eds. Michette, A. G., Morrison, G. R. and Buckley, C. J., Springer-Verlag, Berlin. 417–422.

    Google Scholar 

  • Cheng, P. C., Acharya, R., Lin, T. H., Samarabandu, J. K., Wang, G., Shinozaki, D. M., Berezney, R., Meng, C., Tamg, W. H., Liou, W. S., Tan, T. C., Summers, R. G., Kuang, H. and Musial, C. (1992): 3-D image analysis and visualization in light microscopy and x-ray microtomography, in: Visualization in Biomedical Microscopies, ed. Kniete, A., VCH Publisher, Weinheim, 361–398.

    Google Scholar 

  • Cheng, P. C., Pareddy, D. R., Samarabandu, J. K. and Lin, T. H. (1993): The use of visible and UV confocal microscopy and multi-dimensional image analysis for the study of pachytene chromosomes in maize, Scanning, 15, Sup. III, 17–19.

    Google Scholar 

  • Chu, H. M., Trang, W. H., Lin, T. H. and P. C. Cheng (1990): Optimal 3-D viewing of confocal images, Scanning 13 (I), 37–39.

    Google Scholar 

  • Clark, G. (ed)(1981): Staining Procedures,Williams & Wilkins, Baltimore.

    Google Scholar 

  • Cogswell, C. (1993): High resolution confocal microscopy of phase and amplitude objects, in this volume.

    Google Scholar 

  • Crane, C. F. and Carman, J. G. (1987): Mechanisms of apomixis in Elymus rectisetus from eastern Australia and New Zealand, Amer. J. Bot., 72: 477–496.

    Article  Google Scholar 

  • Cutler, D. F., Alvin, K. L. and Price, C. E. (1982): The Plant Cuticle, Academic Press, New York.

    Google Scholar 

  • Dawe, R. K., Agard, D. A., Sedat, J. W. and Cande, W. Z. (1992): Pachytene DAPI map, Maize Genet. Coop. News Letter, 66: 23–25.

    Google Scholar 

  • Dayanandan, P., Kaufman, P. B. and Franklin, C. I. (1983): Detection of silica in plants, Amer. J. Bot., 70: 1079–1084.

    Google Scholar 

  • Dickinson, H. G. and Bell, P. R. (1972): The role of the tapetum in the formation of sporopollenin-containing structures during microsporogenesis in Pinus banksiana, Planta (Berl.), 107: 205–215.

    Google Scholar 

  • Dohrmann, G. J. and Wick, K. M. (1971): Demonstration of the microvasculature of the spinal cord by intra-venous injection of the fluorescenct dye, thioflavin S, Stain Technol. 46: 321–322.

    PubMed  CAS  Google Scholar 

  • Echlin, P. and Godwin, G. (1968): The ultrastructure and ontogeny of pollen in Heleborus foetidus L., I. The development of the tapetum and Ubisch body, J. Cell Biol., 3: 161–174.

    CAS  Google Scholar 

  • Federikson, M. (1992): The development of the female gemetophyte of Epipactis (Orchidaceae) and its inference for reproductive ecology. Amer. J. Bot., 79: 63–68.

    Article  Google Scholar 

  • Gratton, E. and vandeVen, M. J. (1990): Laser sources for confocal microscopy, in: The Handbook of Biological Confocal Microscopy. ed. J. Pawley, Plenum Press, New York. 53–67.

    Google Scholar 

  • Greenspan, P., Mayer, E. P. and Fowler, S. D. (1985): Nile red: A selective fluorescent stain for intracellular lipid droplets, J. Cell Biol., 100: 965–973.

    Article  PubMed  CAS  Google Scholar 

  • Herr, J. M. Jr. (1971): A new clearing squash technique for the study of ovule development in angers perms, Amer. J. Bot., 58: 785–790.

    Article  Google Scholar 

  • Herr, J. M. Jr. (1974): A clearing-squash technique for the study of ovule and megagametophyte development in angiosperms, in: Vascular Plant Systematics. eds. Radford, A. E., Dickison, W. C., Massey, J. R. and Bell, C. R., Harper & Row, New York.

    Google Scholar 

  • Herr, J. M. Jr. (1985): The removal of phlobaphenes for improved clearing of sections and whole structures, Amer. J. Bot., 72: 817.

    Google Scholar 

  • Herr, J. M. Jr. (1992): Recent advances in clearing techniques for study of ovule and female gametophyte development, in: Angersperm Pollen and Ovules, eds. Ottaviano, E., Mulcahy, W. L., SariGorla, M., and Mulcahy, G. B., Springer-Verlag, NY, 149–154.

    Google Scholar 

  • Heslop-Harrison, J. and Dickinson, H. G. (1969): Time relationship of sporopollenin synthesis associated with tapetum and microspores in Lilium, Planta (Berl.), 84: 199214.

    Google Scholar 

  • Hodson, M. J. and Sangster (1988): Silica deposition in the inflorescence bracts of wheat (Triticum aestivum), I. Scanning electron microscopy and light microscopy, Can. J. Bot., 66: 829–838.

    Article  Google Scholar 

  • Holloway, P. J. and Baker, E. A. (1968): Isolation of plant cuticles with zinc chloride-hydrochloric acid solution, Plant Physiol, 43: 1878–1879.

    Article  PubMed  CAS  Google Scholar 

  • Holloway, P. J. (1982): The chemical constitution of plant cutins, in: The Plant Cuticle. eds. Cutler, D. F., K. L. Alvin and C. E. Price, Academic Press, London, 45–85.

    Google Scholar 

  • Horner, H. T. and Wagner, B. L. (1992): Association of four different calcium crystals in the anther connective tissue and hypodermal stomium of Capsicum annuum (Solanaceae) during microsporogenesis, Amer. J. Bot., 79: 531–541.

    Article  Google Scholar 

  • Hudson, B. and Makin, M. J. (1970): The optimum tilt angle for electron stereo-microscopy. J. Phy. E.: Sci. Instru., 3: 311.

    Article  Google Scholar 

  • Hughes, J. and McCully, M. E. (1975): The use of an optical brightener in the study of plant structure, Stain Technol., 50: 319–329.

    PubMed  CAS  Google Scholar 

  • Johansen, D. A. (1940): Plant Microtechnique, McGraw-Hill, New York.

    Google Scholar 

  • Jones, L. H. P. and Handreck, K. A. (1967): Silica in soils, plants and animals, Adv. Agron., 19: 107–149.

    Article  CAS  Google Scholar 

  • Juniper, B. E. and Jeffree, C. E. (1983): Plant Surfaces, Edward Arnold, London.

    Google Scholar 

  • Kate, T., Tan, K., Balen, R., Groen, F. C. A., Smeulders, W. M. and Boer, G. (1990):

    Google Scholar 

  • SCILAIM: a multilevel interactive image processing system, Pat. Recogn. Lett., 10:429–441.

    Google Scholar 

  • Kim, H., Cheng, P. C., Wittman, M.D. and Kong, H. J. (1989): X-ray microscopy of living biological specimens using a laser-plasma as an x-ray source, Spie, 1140: 256–261.

    Google Scholar 

  • Kim, H., Yaakibi, B., Soures, J. M. and Cheng, P. C. (1992): Laser-produced plasma as a source for x-ray microscopy, in: X-ray microscopy III, eds. Michette, A. G., Morri, G. R. and Buckley, C. J., Springer-Verlag, Berlin, 47–53.

    Google Scholar 

  • Kirk, P. Kl. Jr. (1970): Neutral red as a lipid fluorochrome, Stain Technol., 45: 1–4. Kitagawa, H. (1993): Theory and principal technologies of the laser scanning confocal microscope, in this volume.

    Google Scholar 

  • Kriete, A. (1990): 4-D data acquisition and visualization methods in computer assisted microscopy, Trans. Royal Micr. Soc., 1: 323–326.

    Google Scholar 

  • Kriete, A. and Pepping, T. (1992): Volumetric data representations in microscopy: Applications to confocal and NMR-microimaging, in: Visualization in Biomedical Microscopies. ed. A. Kriete. VCH Publisher, Veinheim, 329–360

    Google Scholar 

  • Kriete, A. (1993): Image quality consideration in computerized 2- D and 3-D microscopy, in this volume.

    Google Scholar 

  • Lechleiter, J. and Clapham, D. (1992): Molecular mechanism of intracellular calcium excitability in X. laevis oocytes, Cell, 69: 283–294.

    Article  PubMed  CAS  Google Scholar 

  • Lide, D. R. (ed)(1991): Handbook of Chemistry and Physics,CRC Press, Boca Raton, 729.

    Google Scholar 

  • Lillie, R. D. and Fullmer, H. M. (1976): Histopathologic Technique and Practical Histochemistry, fourth edition, McGraw-Hill, NY.

    Google Scholar 

  • Liou, W. S., Wang, G., Lin, T. H. and Cheng, P. C. (1992): Deconvolution of optical microscopic images. Scanning 14, Sup II, addendum, 15–17.

    Google Scholar 

  • Newman, W. M. and Sproull, R. E. (1979): Principles of Interactive Computer Graphics, second edition, McGraw-Hill, New York.

    Google Scholar 

  • Oud, J. L. and Nanninga, N. (1992): Cell shape, chromosome orientation and the position of the plane of division in Vicia faba root cortex cells, J. Cell Sci., 103: 847–855.

    Google Scholar 

  • Pace, G. M., Reed, J. N., Ho, L. C. and Fahey, J. W. (1987): Anther culture of maize and the visualization of embiyogenic microspores by fluorescent microscopy, Theor. Appl. Genet., 73: 863–869.

    Article  Google Scholar 

  • Palser, B. F., Rouse, J. L. and Williams, E. G. (1989): Coordinated timetables for megagametophyte development and pollen tube growth in Rhododendron nuttalli from anthesis to early post-fertilization, Amer. J. Bot., 76: 1167–1202.

    Article  Google Scholar 

  • Pareddy, D. R., Greyson, R. I. and Walden, D. B. (1989): Production of normal, germinable and viable pollen from in vitro cultured maize tassels, Theor. Appl. Genet., 77: 521–526.

    Article  Google Scholar 

  • Reijnen, W. H., van Herpen, M. M. A., de Groot, P. F. M., Olmedilla, A., Schrauwen, J. A. M. and Weterings, K. A. P. (1991): Cellular localization of a pollen-specific mRNA by in situ hybridization and confocal laser scanning microscopy, Sex Plant Report, 4: 254257.

    Google Scholar 

  • Russell, W. C., Newman, C. and Williams, D. H. (1975): A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses, Nature, 253: 461–462.

    Article  PubMed  CAS  Google Scholar 

  • Samarabandu, J. K., Acharya, R., Cheng, P. C., Meng, C., Berezney, R. and Summers, R. G. (1991a): Three-dimensional structural analysis from biological confocal images, Spie, 1556, 154–164.

    Article  Google Scholar 

  • Samarabandu, J. K., Acharya, R., Edirisinghe, C. D., Cheng, P. C., Kim, H., Lin, T. H., Summers, R. G. and Musial, C. E. (1991b): Multi-dimensional analysis of confocal images, Spie, 1450, 296–322.

    Article  Google Scholar 

  • Samarabandu, J. K., Acharya, R., and Cheng, P. C. (1993): Analysis and presentation of three dimensional data sets, in this volume.

    Google Scholar 

  • Shaw, P. J. and Rawlins, D. J. (1990): Measurements of the point spread function and its use in deconvolution of confocal microscope images, Trans. Royl. Microsc. Soc., 1: 227230.

    Google Scholar 

  • Sheppard, C. J. R. (1993): Confocal microscopy: basic principles and system performance, in this volume.

    Google Scholar 

  • Shinozald, D. M., Cheng, P. C., Haridoss, S., Mitchell, R. and Fenster, A. (1991): Three-dimensional optical microscopy of water tree in polyethylene, J. Material Sci., 26: 6151–6160.

    Article  Google Scholar 

  • Stelly, D. M., Peloquin, S. J., Palmer, R. G. and Crane, C. F. (1984): Mayer’s hemalummethyl salicylate: a stain-clearing technique for observations within whole ovules. Stain Technol., 59: 155–161.

    PubMed  CAS  Google Scholar 

  • Stelzer, E. H. K. (1993): Designing a cconfocal fluorescence microscope, in this volume

    Google Scholar 

  • Summers R. G., Musial, C. E., Cheng, P. C., Leith, A. and Marko, M. (1991): The use of confocal microscopy and STERECON reconstructions in the analysis of sea urchin embryonic cell division, J. Electron Microscopy Technig. 18: 24–30.

    Article  CAS  Google Scholar 

  • Tsien, R. Y. (1989): Fluorescent probes of cell signalling. Annu. Rev. Neurosci., 12, 227–253.

    Article  PubMed  CAS  Google Scholar 

  • Vergne, P. Delvallee, I. and Dumas, C. (1987): Rapid assessment of microspore and pollen development stages in wheat and maize using DAPI and membrane permeabilization, Stain Technol.,62: 299–304.

    PubMed  CAS  Google Scholar 

  • Young, B. A., Sherwood, R. T. and Bashaw, E. C. (1979): Cleared-pistil and thick-sectioning techniques for detecting aposporous apomixis in grasses, Can. J. Bot., 57: 16681672.

    Google Scholar 

  • Wang, G., Liou, W. S., Lin, T. H. and Cheng, P. C. (1992): A new no-neighbor algorithm for microscopic image restoration, Scanning,14 Sup. H, Addendum, 18–20.

    Google Scholar 

  • Wang, G., Liou, W. S., Lin, T. H. and Cheng, P. C. (1993): Image restoration in light microscopy, in this volume.

    Google Scholar 

  • Wang, G., Liou, W. S., Lin, T. H., and Cheng, P. C. (1993): A model based no-neighbor algorithm for microscopic image deconvolution, Scanning, 15, Sup. III, 64–65.

    Google Scholar 

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Authors
  1. Ping-chin Cheng
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  2. Dayakar R. Pareddy
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  3. Tein-Hsiang Lin
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  4. Jagath K. Samarabandu
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  5. Raj Acharya
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  6. Ge Wang
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  7. Wen Shan Liou
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Editor information

Editors and Affiliations

  1. Advanced Microscopy and Imaging Lab Dept. of Electrical and Computer Engineering, State University of New York at Buffalo, 14260, Buffalo, NY, USA

    P. C. Cheng  & T. H. Lin  & 

  2. Institute of Zoology, Academia Sinica, Taipei, Taiwan, Republic of China

    W. L. Wu  & J. L. Wu  & 

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Cheng, Pc. et al. (1994). Confocal Microscopy of Botanical Specimens. In: Cheng, P.C., Lin, T.H., Wu, W.L., Wu, J.L. (eds) Multidimensional Microscopy. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8366-6_19

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  • DOI: https://doi.org/10.1007/978-1-4613-8366-6_19

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