Confocal Microscopy of Botanical Specimens

  • Ping-chin Cheng
  • Dayakar R. Pareddy
  • Tein-Hsiang Lin
  • Jagath K. Samarabandu
  • Raj Acharya
  • Ge Wang
  • Wen Shan Liou


In general, plant cells are highly heterogeneous with reference to their optical properties, i.e.,absorbance, refractive index, fluorescence, phosphorescence, and birefringence. Cellular organelles and extracellular structures such as light absorbing chloroplasts and pigments, the cuticular layer and waxes found on the surface of epidermal cells, the cell wall, the exine of the pollen grains, and starch, lipid, and protein granules commonly found in the storage tissues all differ significantly from each other and the surrounding aqueous medium in terms of optical properties. This optical heterogeneity creates a major problem in confocal microscopy of plant cells.


Confocal Microscopy Pollen Tube Starch Granule Point Spread Function Confocal Image 
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Copyright information

© Springer-Verlag New York Inc. 1994

Authors and Affiliations

  • Ping-chin Cheng
  • Dayakar R. Pareddy
  • Tein-Hsiang Lin
  • Jagath K. Samarabandu
  • Raj Acharya
  • Ge Wang
  • Wen Shan Liou

There are no affiliations available

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