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Spectrophotometry and Fluorometry in Ion Transport Epithelia

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Book cover Measurement of Ion Transport and Metabolic Rate in Insects

Part of the book series: Springer Series in Experimental Entomology ((SSEXP))

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Abstract

The subject of this chapter is the use of spectrophotometry and fluorometry to monitor the redox state of respiratory enzymes in intact epithelial tissues, with an emphasis on insect epithelia. These optical methods were used initially in isolated mitochondria and later in a variety of intact tissues in vitro and even in vivo. Their main advantages reside in being rapid and noninvasive, the latter allowing the optical monitoring to be performed in conjunction with other types of measurements normally used to assess epithelial function. Spectrophotometry and fluorometry of intact epithelia have provided novel information not obtainable by other methods.

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Authors
  1. Lazaro J. Mandel
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Editors and Affiliations

  1. Department of Developmental and Cell Biology, School of Biological Sciences, University of California, 92717, Irvine, California, USA

    Timothy J. Bradley

  2. Department of Entomology, University of California, 92521, Riverside, California, USA

    Thomas A. Miller

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© 1984 Springer-Verlag New York Inc.

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Mandel, L.J. (1984). Spectrophotometry and Fluorometry in Ion Transport Epithelia. In: Bradley, T.J., Miller, T.A. (eds) Measurement of Ion Transport and Metabolic Rate in Insects. Springer Series in Experimental Entomology. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8239-3_6

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  • DOI: https://doi.org/10.1007/978-1-4613-8239-3_6

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4613-8241-6

  • Online ISBN: 978-1-4613-8239-3

  • eBook Packages: Springer Book Archive

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