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Glomerular Basement Membrane Antigens

  • Alfred J. Fish
  • Kathleen M. Carmody
  • Mark S. Schiffer
  • Alfred E. Michael

Abstract

In experimental nephritis produced by the administration of nephrotoxic serum, there is in vivo localization of heterologous immunoglobulin antibodies in a smooth linear pattern along the glomerular basement membrane (GBM) (Hammer and Dixon, 1963). Nephrotoxic serum, produced by immunization with isolated GBM, reacts in vitro by fixing not only to GBM, but also to tubular basement membrane and to Bowman’s capsule as noted by indirect immunofluorescence. The smooth linear staining of these basement membranes is similar to the GBM staining pattern described above in nephrotoxic serum nephritis. The antigenic specificity of nephrotoxic serum has not been defined but it is believed to be due to a reaction with noncollagenous glycoprotein components of GBM, since multiple precipitin lines are formed in Ouchterlony immunodiffusion against collagenase digests of normal GBM (Marquardt et al., 1973). Another experimental model of glomerulonephritis mediated by anti-GBM antibody can be induced in sheep by immunization with heterologous GBM. The proliferative glomerulonephritis, which was first described by Steblay (1962), is associated with linear deposition of host immunoglobulin along the GBM. In this model of experimental anti-GBM nephritis, circulating antibodies to GBM have been detected after nephrectomy (Lerner et al., 1967).

Keywords

Glomerular Basement Membrane Proliferative Glomerulonephritis Tubular Basement Membrane Glomerular Capillary Wall Dense Deposit Disease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Druet, P., Bariety, J., Bellon, B., and Laliberte, F., 1972, Nephrotoxic serum nephritis in the rat: Ultrastructural localization of nephrotoxic rabbit antibodies using peroxidase-labeled conjugates, Lab. Invest. 27:157.PubMedGoogle Scholar
  2. Duncan, D., Drummond, K. N., Michael, A. F., and Vernier, R. L., 1965, Pulmonary hemorrhage and glomerulonephritis: Report of six cases and study of the renal lesion by fluorescent antibody technique and electron microscopy, Ann. Intern. Med. 62:920.PubMedGoogle Scholar
  3. Fish, A. J., Carmody, K. M., and Michael, A. F., 1979, Distribution of antigens of human glomerular basement membrane, J. Lab. Clin. Med. 94:447.PubMedGoogle Scholar
  4. Hammer, D. K., and Dixon, F. J., 1963, Experimental glomerulonephritis. II. Immunologic events in the pathogenesis of nephrotoxic serum nephritis in the rat, J. Exp. Med. 117:1019.PubMedCrossRefGoogle Scholar
  5. Hoedemaeker, P. J., Feenstra, K., Nijkeuter, A., and Arends, A., 1972, Ultrastructural localization of heterologous nephrotoxic antibody in the glomerular basement membrane of the rat, Lab. Invest. 26:610.PubMedGoogle Scholar
  6. Kim, Y., Vernier, R. L., Fish, A. J., and Michael, A. F., 1979, Immunofluorescent studies of dense deposit disease: The presence of railroad tracks and mesangial rings, Lab. Invest. 40:474.PubMedGoogle Scholar
  7. Lerner, R. A., Glassock, R. J., and Dixon, F. J., 1967, The role of anti-glomerular basement embrane antibody in the pathogenesis of human glomerulone phritis, J. Exp. Med. 126:989.PubMedCrossRefGoogle Scholar
  8. McPhaul, J. J., and Dixon, F. J., 1970, Characteristics of human antiglomerular basement membrane antibodies eluted from glomerulonephritic kidneys, J. Clin. Invest. 49:308.Google Scholar
  9. Marquardt, H., Wilson, C. B., and Dixon, F. J., 1973, Isolation and immunological characterization of human glomerular basement membrane antigens, Kidney Int. 3:57.PubMedCrossRefGoogle Scholar
  10. Miller, K., and Michael, A. F., 1976, Immunopathology of renal extracellular membranes in diabetes mellitus: Specificity of tubular basement membrane fluorescence, Diabetes 25:701.PubMedCrossRefGoogle Scholar
  11. Scheinman, J. I., Fish, A. J., and Michael, A. F., 1974, The immunohistopathology of glomerular antigens: The GBM, collagen and actomyosin antigens in normal and diseased kidneys, J. Clin. Invest. 54:1144.PubMedCrossRefGoogle Scholar
  12. Scheinman, J. I., Fish, A. J., Matas, A. J., and Michael, A. F., 1978, The immunohistopathology of glomerular antigens. II. The glomerular basement membrane, actomyosin and fibroblast surface antigens in normal, diseased and transplanted human kidneys, Am. J. Pathol. 90:71.PubMedGoogle Scholar
  13. Schiffer, M. S., Michael, A. F., Kim, Y., Fish, A. J., 1981, Distribution of glomerular basement membrane antigens in diseased human kidneys, Lab. Invest. 44:234.PubMedGoogle Scholar
  14. Steblay, R. W., 1962, Glomerulonephritis induced in sheep by injections of heterologous glomerular basement membrane and Freund’s complete adjuvant, J. Exp. Med. 116:253.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag US 1983

Authors and Affiliations

  • Alfred J. Fish
    • 1
  • Kathleen M. Carmody
    • 1
  • Mark S. Schiffer
    • 1
  • Alfred E. Michael
    • 1
  1. 1.Department of PediatricsUniversity of Minnesota Medical SchoolMinneapolisUSA

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