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Synthesis of Pure Polypeptide Hormones with Full Biological Activity

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The Chemistry of Polypeptides

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Abstract

In the synthesis and isolation of naturally occurring macromolecules, especially polypeptides, the two major methods of purification employed in organic synthesis, namely, recrystallization and fractional distillation, are usually not applicable. Only in the so-called lower region of smaller peptides or in a very few special cases is it possible to obtain pure materials by crystallization; in the so-called upper region of larger peptides, crystallization is successful only if preceded by other methods of purification. The present situation of research into the purification of polypeptide hormones can be comprehensively considered using as examples the total syntheses of the hormones glucagon, secretin, and [leucine-15]-human gastrin I, an analogue of the natural product.

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Authors and Affiliations

  1. Abteilung für Peptidchemie, Max-Planck-Institut für Biochemie, München, Germany

    Erich Wünsch

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  1. Erich Wünsch
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Editors and Affiliations

  1. Department of Biochemistry, Mount Sinai School of Medicine, City University of New York, New York, New York, USA

    P. G. Katsoyannis (Chairman) (Chairman)

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© 1973 Plenum Press, New York

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Wünsch, E. (1973). Synthesis of Pure Polypeptide Hormones with Full Biological Activity. In: Katsoyannis, P.G. (eds) The Chemistry of Polypeptides. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-4571-8_13

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  • DOI: https://doi.org/10.1007/978-1-4613-4571-8_13

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4573-2

  • Online ISBN: 978-1-4613-4571-8

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