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Types of Size Heterogeneity of IgA Molecules Isolated from Myeloma and Normal Human Serum

  • J. M. Fine
  • P. Lambin
  • D. Frommel

Abstract

In normal human serum IgA is present in a series of polymeric forms; the largely predominant monomeric 7S form coexists with components of increasing sedimentation coefficients (1,2). This size heterogeneity was also observed in serum of IgA myeloma (3,4). In 1964, studying 32 isolated IgA myeloma proteins, we reported that starch gel electrophoresis revealed 3 different types of polymerization — type I, II and III (5). More than 95% of 165 IgA M-components analyzed in this laboratory could be ascribed to one of these types. In our experience the type of polymerization remains stable for any given IgA myeloma. Taking advantage of a procedure for IgA purification which avoids the sieving effects or gel filtration (6), we confirmed and extended our previous findings (7). In this communication we present data concerning the types of size heterogeneity observed in monoclonal and polyclonal IgA preparations.

Keywords

Normal Human Serum Size Heterogeneity Immunochemical Analysis Idiotypic Determinant Analytical Ultra 
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References

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    Fine, J.M. , Lambin, P. and Frommel, D., Biomedicine 18: 145, 1973.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • J. M. Fine
    • 1
  • P. Lambin
    • 1
  • D. Frommel
    • 1
  1. 1.Centre National de TransfusionParisFrance

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