Conversion of φX174 and fd DNA to Their Replicative Forms by Two Enzyme Systems in Escherichia coli

  • S. Wickner
  • R. B. Wickner
  • M. Wright
  • I. Berkower
  • J. Hurwitz
Part of the Basic Life Sciences book series (BLSC, volume 3)

Abstract

Extracts of Escherichia coli pol Al strains catalyze the conversion of φX174 or fd (M13) single-stranded circular DNA to the replicative form (1,2,). The fd DNA dependent reaction is sensitive to rifampicin and requires all four ribo- nucleoside triphosphates, but does not involve the products of the dnaA or dnaB gene. The reaction with φX174 DNA, in contrast, is rifampicin resistant and is temperature sensitive in extracts of dnaBts cells (1,2).

Keywords

Glycerol Lysozyme Rifampicin Triphosphate Thiamine 

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • S. Wickner
    • 1
  • R. B. Wickner
    • 1
  • M. Wright
    • 1
  • I. Berkower
    • 1
  • J. Hurwitz
    • 1
  1. 1.Department of Developmental Biology and CancerAlbert Einstein College of MedicineBronxUSA

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