Riboflavin pp 81-98 | Cite as

Chromatographic and Radioisotopic Methods for the Analysis of Riboflavin and the Flavin Coenzymes

  • Arpad G. Fazekas


This chapter will describe chromatographic and radioisotopic methods for the analysis of riboflavin (RF) and the flavin coenzymes in animal tissues. Chromatographic separations of the three major tissue flavins—flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN), and RF—have been in wide use since the early 1950s and are summarized in excellent review articles(21) and textbooks.(16,14) The first part of this chapter, therefore, deals only with the most important and practical chromatographic methods, including those that have been successfully used in the author’s laboratory.


Flavin Adenine Dinucleotide Flavin Mononucleotide Radioisotopic Method Ammonium Sulfate Solution Thin Layer Chromatographic Separation 
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Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Arpad G. Fazekas
    • 1
  1. 1.Department of MedicineUniversity of Montreal and Laboratory of Endocrinology, Notre-Dame HospitalMontrealCanada

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