Genetic Control of the Immune Response to Ordered Peptides of Tyrosine and Glutamic Acid
One of the important problems in understanding the genetic regulation of the immune response potential to random synthetic polypeptides is to establish the major determinant(s) of all possible amino acid combinations which is responsible for the phenotypic expression of the immune response to the whole immunogen. The random synthetic immunogen poly(LTyr, LGlu)-poly(DLAla)—poly(LLys) abbreviated (T, G)-A—L is one of the antigens most extensively used in studies of the genetic control of the immune responses. Therefore, in order to elucidate the above-stated problem we have prepared several ordered tetrapeptides composed of tyrosine and glutamic acid which were attached to multichain poly-DL-alanine (A—L) Only one of the antigenic determinants, namely Tyr-Tyr-Glu-Glu, resembled the random peptide (T,G) in the pattern of immune responses elicited against it and in the cross-reactivity of the specific antibodies with (T,G)-A—L. The immune response pattern to the other ordered tetrapeptides tested was different from that obtained with (T,G)-A—L, and no cross-reactivity was detected between the antibodies provoked with these peptides and (T,G)-A—L (1). The gene(s) controlling the ability to respond to the random polypeptide (T,G)-A—L was found to be linked to the major histocompatibility (H-2) locus of the mouse (2).