Chemotherapy pp 335-340 | Cite as

In Vitro Methods for Use in Drug Metabolism Studies

  • B. J. Phillips
  • P. J. Cox


Cyclophosphamide is a potent and relatively specific anti-tumour alkylating agent which requires activation by liver microsomal enzymes. The drug has been under intensive study for many years and the general outline of its metabolism has been largely worked out (for a review see ref. 1). The purpose of this paper is to describe some cell culture techniques which were used as part of an extensive study of cyclophosphamide metabolism carried out at the Chester Beatty Research Institute, London. The contribution of these methods to the understanding of cyclophosphamide metabolism, and their potential usefulness in drug metabolism studies in general, will be discussed.


Secondary Oxidation Cell Culture Technique Phosphoramide Mustard Vitro Method NADPH Generate System 
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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • B. J. Phillips
    • 1
  • P. J. Cox
    • 1
  1. 1.Chester Beatty Research InstituteLondonUK

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