Recent Experience with Microbial Systems and Cancer Cells in Vitro in the Screening for Antitumor Antibiotics
New mutants of E. coli with altered cytoplasmic membrane and increased permeability have been profitably employed for the detection of new antimetabolites synthesized by microorganisms. Mitochondrial mutants of yeast also represent considerable interest in the screening for new antimetabolites synthesized by actinomycetes, since they can detect products which remain unnoticed with the aid of many other tests. Ascites tumor cells, after numerous alternating passages through the primary culture in the test tube and through the abdominal cavity of mice, are also useful in the screening of antitumor antibiotics.
KeywordsTest Tube Minal Cavity Cell Wall Biosynthesis Microbial System Microbiological Assay
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