Abstract
Guanosine 3′,5′-monophosphate (cGMP) has been detected in most animal (11) and plant (12) tissues and in bacteria (1). Its concentration generally varies between 10-8 and 10-7 mol/kg wet weight, although in some cases it can be as high as 10-6 mol/kg (11). This is very low compared to the concentrations of related nucleotides and of cAMP.
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BERNLOHR, R.W., HADDOX, M.K. and GOLDBERG, N.D. (1974) Cyclic guanosine 3’:5’-monophosphate in Escherichia coli and Bacillus licheniformis, J. Biol. Chem. 249, 4329
BÖHME, E., and SCHULTZ, G., (1974), Separation of cyclic nucleotides by thin layer chromatography on polyethyleneimine cellulose, in “Methods in Enzymology”, ed. by S.P. Colowick and N.O. Kaplan, 38, 27
BROOKER, G. (1972), High pressure anion exchange chromatography and enzymatic isotope displacement assays for cyclic AMP and cyclic GMP, Adv. Cyclic Nucl. Research 2, 111
BROOKER, G., THOMAS, L.J. and APPLEMAN, M.M. (1968), The assay of adenosine 3’:5’-cyclic monophosphate and guanosine 3’:5’-cyclic monophosphate in biological materials by enzymatic radioisotopic displacement, Biochemistry 7, 4177
CAILLA, H.L., , M.S. and DeLAAGE, M.A. (1973), Adenosine 3’,5’ cyclic monophosphate assay at 10-15 mole level. Analyt. Biochem. 56, 394
COOPER, R.H., ASHCROFT, S.J.H., and RANDLE, P.J. (1973) Concentration of adenosine 3’:5’-cyclic monophosphate in mouse pancreatic islets measured by a protein-binding radioassay. Biochem. J. 134, 599
DINNENDAHL, V. (1974) A rapid and simple procedure for the determination of guanosine 3’, 5’-monophosphate by use of the protein-binding method. Naunyn-Schmiedeberg’s Arch. Pharmacol. 284, 55
FLOURET, G., and HECHTER, O (1974) Adsorption chromatography of cyclic nucleotides on silica gel and alumina thin-layer sheets. Analyt. Biochem. 58, 276
GOLDBERG, N.D., DIETZ, S.B. and O’TOOLE, A.G. (1969) Cyclic guanosine 3’:5’-monophosphate in mammalian tissues and urine J. Biol. Chem. 244, 4458
GOLDBERG, N.D. and O’TOOLE, A.G. (1971) Analysis of cyclic 3’,5’-adenosine monophosphate and cyclic 3’5’-guanosine monophosphate, in “Methods Biochem. Anal.”, ed. by D. Glick, Vol. 20, 1
GOLDBERG, N.D., O’DEA, R.F. and HADDOX, M.K. (1973), Cyclic GMP, Adv. Cycl. Nucl. Res. 3, 155
HADDOX, M.K., STEPHENSON, J.H., and GOLDBERG, N.D. (1974) Cyclic GMP in meristematic and elongating regions of bean root. Fed. Proc. 33, 522
HARDMAN, J.G., DAVIS, J.W. and SUTHERLAND, E.W. (1966). Measurement of guanosine 3’, 5’-monophosphate and other cyclic nucleotides. Variations in urinary excretion with hormonal state of the rat. J. Biol. Chem. 241, 4812
HARDMAN, J.G., DAVIS, J.W. and SUTHERLAND, E.W. (1969). Effects of some hormonal and other facts on the excretion of guanosine 3’, 5’-monophosphate and adenosine 3’,5’-monophosphate in rat urine. J. Biol. Chem. 244, 6354
JAKOBS, K.H., BÖHME, E., MOCIKAT, S. (1974). Cyclic GMP formation in human platelets. Naunyn-Schmiedeberg’s Arch. Pharmacol. 282, R 40
JAKOBS, K.H., BÖHME, E., SCHULTZ, G. (1974). Determination of cyclic GMP and cyclic AMP in human platelets. Second International Conference on Cyclic AMP, Vancouver 1974, Abstracts, 51
KRISHNA, G., WEISS, B. and BRODIE, B.B. (1968) A simple sensitive method for the assay of adenyl cyclase. J. Pharmacol, exper. Therap. 163, 379
KUO, J.F., and GREENGARD, P. (1970) Cyclic nucleotide-dependent protein kinases. VI. Isolation and partial purification of a protein kinase activated by guanosine 3’,5’- monophosphate. J. Biol. Chem. 245, 2493
KUO, J.F., WYATT, G.R. and GREENGARD, P. (1971) Cyclic nucleotide-dependent protein kinases. IX. Partial purification and some properties of guanosine 3’,5’-monophosphate-dependent and adenosine 3’,5’-monophosphate-dependent protein kinases from various tissues and species of arthropoda. J. Biol. Chem. 246, 7159
KUO, J.F., LEE, T.P., REYES, P.L., WALTON, K.G. DONNELLY, T.E., and GREENGARD, P. (1972) Cyclic nucleotide-dependent protein kinases. X. Assay method for measurement of guanosine 3’,5’-monophosphate in various biological materials and a study of agents regulating its levels in heart and brain. J. Biol. Chem. 247, 16
MAO, C.C., and GUIDOTTI, A. (1974) Simultaneous isolation of adenosine 3’,5’-cyclic monophosphate (cAMP) and guanosine 3’,5’-cyclic monophosphate (cGMP) in small tissue samples. Analyt. Biochem. 59, 63
MURAD, F., MANGANIELLO, V., and VAUGHAN, M. (1971). A simple, sensitive protein-binding assay for guanosine 3’,5’-monophosphate. Proc.Nat.Acad.Sci. US, 88, 736
NAKAZAWA, K., and SANO, M. (1974). Studies on guanylate cyclase. A new assay method for guanylate cyclase and properties of the cyclase from rat brain. J. Biol. Chem. 249, 4207
SCHMIDT, M.J., SCHMIDT, D.E., and ROBISON, G.A. (1971) Cyclic adenosine monophosphate in brain areas: Microwave irradiation as a means of tissue fixation. Science 173, 1142
SCHULTZ, G., HARDMAN, J.G., SCHULTZ, K., DAVIS, J.W. and SUTHERLAND, E.W. (1973). A new enzymatic assay for guanosine 3’:5’-cyclic monophosphate and its application to the ductus deferens of the rat. Proc.Nat.Acad.Sci. US, 70, 1721
SCHULTZ, G., BÖHME, E., and HARDMAN, J.G. (1974). Separation and purification of cyclic nucleotides by ion-exchange resin column chromatography, in “Methods in Enzymology”, ed. by S.P. Colowick and N.O. Kaplan, 38, 9
SCHULTZ, G., and HARDMAN, J.G. (1974). Determination of cyclic GMP by formation of (ß-32P) GDP, in “Methods in Enzymology”, ed. by S.P. Colowick and N.O. Kaplan, 38, 106
STEINER, A.L., KIPNIS, D.M., UTIGER, R.D. and PARKER, C.W. (1969), Radioimmunoassay for the measurement of adenosine 3’,5’-cyclic phosphate. Proc. Nat. Acad. Sci. US, 64, 367
STEINER, A.L., PARKET, C.W. and KIPNIS, D.M. (1972) Radioimmunoassay for cyclic nucleotides. I. Preparation of antibodies and iodinated cyclic nucleotides. J. Biol. Chem. 247, 1106
TSUBOI, K.K., and PRICE, T.D. (1959). Isolation, detection and measure of microgram quantities of labeled tissue nucleotides. Arch. Biochem. Biophys. 81, 223
WHITE, A.A., ZENSER, T.V. (1971) Separation of cyclic 3’,5’-nucleotide monophosphates from other nucleotides on aluminum oxide columns. Application to the assay of adenyl cyclase and guanyl cyclase. Analyt. Biochem. 41, 372
WUNDERWALD, P., and MICHAL, G. (1974), personal communication
ZETTNER, A., and DULY, P.E. (1974). Principles of competitive binding assays (Saturation analyses). II. Sequential saturation. Clin. Chem. 20, 5
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Jakobs, K.H., Böhme, E., Schultz, G. (1976). Determination of Cyclic GMP in Biological Material. In: Dumont, J.E., Brown, B.L., Marshall, N.J. (eds) Eukaryotic Cell Function and Growth. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-4322-6_14
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DOI: https://doi.org/10.1007/978-1-4613-4322-6_14
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