Human Phosphoribosylpyrophosphate Synthetase: Relation of Activity and Quaternary Structure

  • Michael A. Becker
  • Laurence J. Meyer
  • William H. Huisman
  • Cheri S. Lazar
  • William B. Adams
Part of the Advances in Experimental Medicine and Biology book series

Abstract

Evidence from a variety of biochemical, pharmacological and clinical studies indicates that the intracellular concentration of 5-phosphoribosyl 1-pyrophosphate (PP-ribose-P)1 is an important determinant of the rate of purine nucleotide and thus uric acid synthesis (Reviews, references 1,2). PP-Ribose-P formation (Figure 1) from ATP and ribose-5-phosphate is catalyzed by the enzyme PP-ribose-P synthetase in a reaction requiring inorganic phosphate (Pi) and magnesium. Small molecule inhibitors also affect PP-ribose-P synthetase activity and include purine, pyrimidine and pyridine nucleotides as well as 2,3-diphosphoglycerate (2,3-DPG) (3). The significance of regulation of the activity of this enzyme is apparent in several families in whom purine overproduction and clinical gout result from different structural mutations in PP-ribose-P synthetase which lead to excessive enzyme activity and PP-ribose-P generation (4–6).

Keywords

Sucrose Magnesium Sedimentation Arginine Catalase 

Abbreviations

PP-ribose-P

5-phosphoribosyl 1-pyrophosphate

ribose-5-P

ribose-5-phosphate

Pi

inorganic phosphate

2, 3-DPG

2, 3-diphosphoglycerate

SDS

sodium dodecylsulfate

DTT

dithiothreitol

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Michael A. Becker
    • 1
  • Laurence J. Meyer
    • 1
  • William H. Huisman
    • 1
  • Cheri S. Lazar
    • 1
  • William B. Adams
    • 1
  1. 1.Department of MedicineUniversity of California, San Diego and San Diego Veterans Administration HospitalLa JollaUSA

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