Localization of Xanthine Oxidase Activity in Hepatic Tissue
A histochemical method of localization of xantine oxidase activiy in glutaraldehyde-fixed rat jejunum had been reported by Sackler (1). The reaction is carried out by the reduction of nitro-blue tetrazolium (NBT) into insoluble formazan, in phosphate buffer with hypoxanthine as substrate. Though Picklett et Al (2) have introduced a modification in fixing the sections, that histochemical technique is ineffective to demonstrate xanthine oxidase activity in rat liver. On the opposite side, biochemical methods can show this enzyme activity. The inability to demonstrate the hepatic enzyme is believed to depend on its lower concentration in liver as to compare to that of the jejunal mucosa (1).
KeywordsToluene Aldehyde Boiling Purine Tetrazolium
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- (2).Picklett J.P, Pendergrass R.E., Bradford W.D. and Elchepp J.G. Localization of xanthine oxidase in rat duodenum. Fixation of section instead of blocks. Stain Techn. 1970, 45, 35–36.Google Scholar