Localization of Xanthine Oxidase Activity in Hepatic Tissue

A New Histochemical Method
  • C. Auscher
  • N. Amory
  • C. Pasquier
  • F. Delbarre
Part of the Advances in Experimental Medicine and Biology book series

Abstract

A histochemical method of localization of xantine oxidase activiy in glutaraldehyde-fixed rat jejunum had been reported by Sackler (1). The reaction is carried out by the reduction of nitro-blue tetrazolium (NBT) into insoluble formazan, in phosphate buffer with hypoxanthine as substrate. Though Picklett et Al (2) have introduced a modification in fixing the sections, that histochemical technique is ineffective to demonstrate xanthine oxidase activity in rat liver. On the opposite side, biochemical methods can show this enzyme activity. The inability to demonstrate the hepatic enzyme is believed to depend on its lower concentration in liver as to compare to that of the jejunal mucosa (1).

Keywords

Toluene Aldehyde Boiling Purine Tetrazolium 

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References

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • C. Auscher
    • 1
  • N. Amory
    • 1
  • C. Pasquier
    • 1
  • F. Delbarre
    • 1
  1. 1.Institut de Rhumatologie, Centre de Recherches sur les Maladies osteoarticulairesU.5 INSERM;ERA 337 CNRSParisFrance

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