Studies on the Effect of Fructose and Xylitol in the Rat Liver: 5′-Nucleotidase, Adenosine Deaminase, De Novo Purine Synthesis

  • J. D. Schwarzmeier
  • M. M. Müller
  • W. Marktl
Part of the Advances in Experimental Medicine and Biology book series

Abstract

Fructose as well as xylitol are both rapidly metabolized in liver the main product being glucose (1, 11). In high doses these sugar surrogates induce an increase of uric acid levels both on parenteral and oral application (4, 5, 8, 14, 16, 18, 20). The causes underlying the hyperuricemia thus produced are still unclear. Since the metabolism of both substrates involves a phosphorylation step, the liver cell ATP concentration is (at least temporarily) reduced. This reduction is accompanied by a depletion of ADP, AMP and inorganic phosphates. The loss of inorganic phosphates (Pi) is thought to activate catabolic enzyme reactions of purine metabolism which, in turn, would enhance purine nucleotide degradation (2, 5, 12, 17 – 20). If this hypothesis is correct, both fructose and xylitol should be expected to activate catabolic enzymes, such as 5′-nucleotidase and adenosine deaminase. This, in turn, would result in a secondary increase of de novo purine synthesis. To validate this assumption we studied the effects of the two sugar alcohols on 5′-nucleotidase and adenosine deaminase activities in the rat liver in vivo and in vitro. At the same time, the incorporation of the purine precursor glycine in rat liver adenine nucleotides was measured.

Keywords

Sugar Catheter Saccharose Glycine Adenosine 

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References

  1. 1.
    Bässler, K. H., Stein, G., Belzer, W.: Biochem. Z. 346, 171 (1966)Google Scholar
  2. 2.
    Bode, L., Schumacher, H., Goebell, H., Zelder, O., Pelzel, H.: Horm. Metab. Res. 3, 71 (1971)CrossRefGoogle Scholar
  3. 3.
    Brand, K.: In: Bergmeyer, H. V.: Methoden der enzymatischen Analyse, 3rd ed., p. 672, Verlag Chemie, Weinheim, 1974Google Scholar
  4. 4.
    Förster, H., Boecker, St., Ziege, M.: Med. u. Ernähr. 13, 193 (1972)Google Scholar
  5. 5.
    Fox, I. H., Kelley, W. N.: Metabolism 21, 713 (1972)PubMedCrossRefGoogle Scholar
  6. 6.
    Gerlach, U., Hiby, W.: In: Bergmeyer, H. V.: Methoden der enzymatischen Analyse, 3rd ed., p.903 Verlag Chemie, Weinheim, 1974Google Scholar
  7. 7.
    Giusti, G.: In: Bergmeyer, H. V.: Methoden der enzymatischen Analyse, 3rd ed., p. 1134, Verlag Chemie, Weinheim, 1974Google Scholar
  8. 8.
    Heuckenkamp, P. V., Zöllner, N.: Lancet II, 808 (1971)CrossRefGoogle Scholar
  9. 9.
    Horecker, B. L., Smyrniotis, P., Hurwitz, J.: J. biol. Chem. 223, 1009 (1956)PubMedGoogle Scholar
  10. 10.
    Kelley, W. N., Rosenbloom, F. M., Henderson, J. F., Seegmiller, J. E.: Proc. Nat. Acad. Sci (USA) 57, 1735 (1967)CrossRefGoogle Scholar
  11. 11.
    Leuthardt, F., Stuhlfauth, K.: Biochemische, physiologische und klinische Probleme des Fructosestoffwechsels. Thieme, Stuttgart, 1960Google Scholar
  12. 12.
    Mäenpää, P. H., Raivio, K. O., Kekomäki, M. P.: Science 161, 1253 (1968)PubMedCrossRefGoogle Scholar
  13. 13.
    Marko, P., Gerlach, E., Zimmer, H. G., Pechan, I., Cremer, Th., Trendelenburg, C.: Hoppe-Seylers Z. physiol. Chem. 350, 1669 (1969)PubMedCrossRefGoogle Scholar
  14. 14.
    Perheentupa, J., Raivio, K. O.: Lancet II, 528 (1967)CrossRefGoogle Scholar
  15. 15.
    Rothman, I. K., Zansani, E. D., Gordon, A. S., Silber, R.: J. Clin. Invest. 49, 2051 (1970)PubMedCrossRefGoogle Scholar
  16. 16.
    Sahebjami, H., Scalettar, R.: Lancet I, 366 (1971)CrossRefGoogle Scholar
  17. 17.
    Schwarzmeier, J. D., Marktl, W., Moser, K., Lujif, A.: Res. exp. Med. 162, 341 (1974)CrossRefGoogle Scholar
  18. 18.
    Woods, H. F., Alberti, K. G. M. M.: Lancet II, 1354 (1972)CrossRefGoogle Scholar
  19. 19.
    Woods, H. F., Krebs, H. A.: Biochem. J. 134, 437 (1973)PubMedGoogle Scholar
  20. 20.
    Woods, H. F.: Nutr. Metabol. 18 (Suppl. 1), 65 (1975)CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • J. D. Schwarzmeier
    • 1
    • 2
    • 3
  • M. M. Müller
    • 1
    • 2
    • 3
  • W. Marktl
    • 1
    • 2
    • 3
  1. 1.1st Dept. of MedicineUniversity of ViennaAustria
  2. 2.1st Dept. of Medical ChemistryUniversity of ViennaAustria
  3. 3.Dept. of PhysiologyUniversity of ViennaAustria

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