Abstract
Adenosine deaminase (ADA) catalyzes the irreversible hydrolytic deamination of adenosine to produce inosine and ammonia (1). Extremely reduced or absent ADA activity in man is associated with one type of severe combined immunodeficiency (SCID) (2). Previous studies of normal ADA from a variety of species has provided evidence for substantial molecular heterogeneity. Multiple forms of ADA varying in molecular weight from 30,000 to 47,000 (designated small form) and 230,000 to 440,000 (designated large form) have been reported. (3–16).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Conway, E.J., and Cooke, R. (1939) Biochem. J. 331, 479–492.
Giblett, E.R., Anderson, J.E., Cohen, F., Pollara, B., and Meuwissen, H.J. (1972) Lancet 2, 1067–1069.
Ma, P.F., and Fisher, J.R. (1968) Comp. Biochem. Physiol. 27, 105–112.
Ma, P.F., and Fisher, J.R.(1968) Comp. Biochem. Physiol. 27, 687–694.
Ma, P.F., and Fisher, J.R.(1968) Biochem. Physiol. Acta 159, 153–159.
Murphy, P.M., Noonan, M., Collins, P., Tully, E., and Brady, T.G. (1969) Biochim. Biophys. Acta 171, 157–166.
Brady, T.G., and O’Connell, W. (1962) Biochim. Biophys. Acta 62216–229.
Pfrogner, N. (1967) Arch. Biochem. Biophys. 119, 141–146.
Cory, J.G., Weinbaum, G., and Suhadolnik, R.J. (1967) Arch. Biochem. Biophys. 118, 428–433.
Spencer, N., Hopkinson, D.A., and Harris, H. (1968) Ann. Hum. Genet. 32, 9–14.
Ressler, N. (1969) Clin. Chim. Acta 24, 247–251.
Ma, P.F., and Fisher, J.R. (1969) Comp. Biochem. Physiol. 31, 771–781.
Akedo, H., Nishihara, H., Shinkai, K., and Komatsu, K., and Ishikawa, S. (1972) Biochim. Biophys. Acta 276, 257–271.
Osborne, W.R.A., and Spencer, N. (1973) Biochem. J. 133, 117–123.
Van der Weyden, M.B., and Kelley, W.N. (1976) J. Biol. Chem., in press.
Piggott, C. O., and Brady, T.G. (1976) Biochim. Biophys. Acta 429, 600–607.
Van der Weyden, M.B., Buckley, R.H., and Kelley, W.N. (1974) Biochem Biophys. Res. Commun. 57, 590–595.
Hirschhorn, R., Beratis, N. and Rosen, R.S. (1975). Proc. Nat. Acad. Sci. USA. 73. 213–217.
Chen, S., Scott, C.R. and Swedberg, K.R., (1975). Am. J. Hum. Genet. 27, 46–52.
Rossi, C.A., Lucacchini, A., Montali, U., and Ronca, G. (1975) Int. J. Peptide Res. 7, 81–89.
Daddona, P.E., and Kelley, W.N. (1976) J. Biol. Chem., in press.
Davis, B.J. (1964) Ann. N.Y. Acad. Sci. 121, 404–427.
Vesterberg, o., and Svensson, H. (1966) Acta Chem. Scand. 20. 820–834.
Cuatrecasas, P. (1970) J. Biol. Chem. 245, 3059–3065.
Gilham, P.T., (1970) Methods Enzymol. 21, 191–197.
Stevenson, K.J. and Landman, A. (1971) Can. J. Biochem. 49. 119–126.
Weber, K., and Osborn, M. (1969) J. Biol. Chem. 244, 4406–4412.
Segrest, J.P. and Jackson, R.L. (1972) Methods Enzymol 28. 54–63.
Ackers, G.K. (1967) J. Biol. Chem. 242, 3237–3238.
McCarty, K.S., Stafford, D., and Brown, O. (1968) Anal. Biochem. 24, 314–329.
Cohn, E.J. and Edsall, J.T. (1943) in “Proteins, Amino Acids, and Peptides”, pp. 370, Reinhold Publishing, New York.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1977 Plenum Press, New York
About this chapter
Cite this chapter
Daddona, P.E., Kelley, W.N. (1977). Purification of Human Erythrocyte Adenosine Deaminase. In: Müller, M.M., Kaiser, E., Seegmiller, J.E. (eds) Purine Metabolism in Man—II. Advances in Experimental Medicine and Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-4223-6_28
Download citation
DOI: https://doi.org/10.1007/978-1-4613-4223-6_28
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-4225-0
Online ISBN: 978-1-4613-4223-6
eBook Packages: Springer Book Archive