Isoelectric Focusing of Microbial Proteins

  • William W. Yotis
Part of the Biological Separations book series (BIOSEP)

Abstract

The application of isoelectric focusing for the isolation and characterization of microbial proteins is increasing slowly. While such methods as electrophoresis, chromatography, ultracentrifugation, as well as fractionation of microbial proteins by salts and organic solvents are all occupying a prominent place in analytical biochemistry, today isoelectric focusing ranks as one of the leading tools in the analytical laboratory. The real strength of isoelectric focusing lies in its ability to provide separation, quantitation, and characterization of quite closely related amphoteric compounds. Proteins varying by as little as 0.02 pH units at their isoelectric point have been clearly resolved.

Keywords

Electrophoresis Fructose Streptomyces Milo Saccharomyces 

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References

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • William W. Yotis
    • 1
  1. 1.Department of Microbiology, Stritch School of MedicineLoyola University of ChicagoMaywood

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