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Isoelectric Focusing of Membrane Components

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Biological and Biomedical Applications of Isoelectric Focusing

Part of the book series: Biological Separations ((BIOSEP))

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Abstract

Three distinctive approaches are now available for the high-resolution, analytical fractionation of membrane proteins. These are: (1) Dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE), where proteins are separated as SDS complexes according to molecular weight (Fairbanks et al., 1971; Weber and Osborn, 1969); (2) crossed immune electrophoresis (CIE), where the proteins, solubilized in nonionic detergent, are separated in one dimension according to charge and in a second dimension according to their antigenic properties (Bjerrum and Bøg-Hansen, 1976); and (3) Isoelectric focusing (IF), where the proteins, solubilized in nonionic detergent, are separated in a pH gradient, according to their isoelectric points.

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Authors and Affiliations

    Authors
    1. Rupert Schmidt-Ullrich
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    2. Donald F. H. Wallach
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    Editor information

    Editors and Affiliations

    1. Massachusetts Institute of Technology, Cambridge, Massachusetts

      Nicholas Catsimpoolas

    2. Tufts University School of Medicine, Boston, Massachusetts

      James Drysdale

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    © 1977 Plenum Press, New York

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    Schmidt-Ullrich, R., Wallach, D.F.H. (1977). Isoelectric Focusing of Membrane Components. In: Catsimpoolas, N., Drysdale, J. (eds) Biological and Biomedical Applications of Isoelectric Focusing. Biological Separations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-4181-9_7

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    • DOI: https://doi.org/10.1007/978-1-4613-4181-9_7

    • Publisher Name: Springer, Boston, MA

    • Print ISBN: 978-1-4613-4183-3

    • Online ISBN: 978-1-4613-4181-9

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