GABA Receptors and Phospholipids
The sodium-independent binding of GABA to certain membrane preparations from rat brain exhibits many of the properties of the interaction of GABA with postsynaptic receptors anticipated from in vivo studies (see papers by Enna and Olsen, this meeting). Enna and Snyder (1975) found that, while sodium-independent GABA binding could be detected in homogenates of fresh brain tissue, freezing and thawing the membrane preparation led to a 2-fold increase in observed sodium-independent binding. Subsequently these authors reported that treatment of the membranes with 0.05% Triton X-100 further increased binding up to 5-fold, and also increased the apparent potencies of various GABA agonists (Enna and Snyder, 1977). Wong and Horng (1977) reported that treatment with 0.5% Triton X-100 increased both the apparent affinity and the apparent density of sodium-independent GABA binding from the values obtained using membranes prepared by extensive freezing and thawing.
KeywordsMembrane Preparation Gaba Receptor Junctional Complex Gaba Agonist Fatty Acid Substituent
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