Abstract
The introduction of intracellular staining methods in neurobiology has provided possibilities for extensive light and electron microscopical studies of single neurones. Intracellular administration of horseradish peroxidase (Jankowska et al., 1976; Snow et al., 1976) has, in this respect, proven superior to other intracellular stains, since the enzyme rapidly distributes in neuronal processes visualizing both dendritic and axonal arborizations, including terminals of initial axon collaterals (Cullheim and Kellerth, 1976; Rastad, 1976; Rastad et al., in press). Thus ultrastructural investigations of initial axon collaterals of lumbar feline spinocervical tract (SCT) cells have demonstrated boutons with axo-dendritic, axo-somatic and axo-axonal synapses (Rastad, 1976; Rastad et al., in press) situated in the same or neighbouring segments of the spinal cord. The purposes of the present study were to investigate the appearance of axodendritic initial collateral terminals of a feline spinocervical tract cell, stained intracellularly with horseradish peroxidase (HRP), and to quantitatively analyse the extent of ultrastructural differences between terminals originating from one functionally characterized neurone.
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Rastad, J. (1978). Ultrastructural Analysis of Axo-Dendritic Initial Collateral Terminals of a Feline Spinocervical Tract Neurone, Stained Intracellularly with Horseradish Peroxidase. In: Fonnum, F. (eds) Amino Acids as Chemical Transmitters. NATO Advanced Study Institutes Series, vol 16. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-4030-0_3
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