Abstract
Direct chemical ionization mass spectrometry (CI-MS) permits the determination of all common free fatty acids and amino acids in blood down to a 25 ng range at a signal/noise ratio better than 10:1 (1). Other low molecular weight blood constituents such as cholesterol, cholesterol esters, triglycerides and other organic acids which may be present in the mass spectra do not interfere with the analysis. Underivatized amino acids, peptides and other blood constituents are either insoluble in the extraction solvent or not sufficiently volatile for direct mass spectrometry.
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References
A. Frigerio and E.L. Ghisalberti (Eds.), “Mass Spectrometry in Drug Metabolism”, Plenum Press, New York, 1977.
J. Diekman and C. Djerassi, J. Org. Chem., 1967, 32, 1005.
J.M.L. Mee, J. Korth and B. Halpern, Clin. Chim. Acta, 1977, in press.
T. Murata, S. Takahashi and T. Takeda, Anal. Chem., 1975, 47, 577.
L.F. Fieser and W.F. Schneider, J. Am. Chem. Soc., 1952, 74, 2254.
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© 1978 Plenum Press, New York
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Mee, J.M.L., Halpern, B. (1978). Quantitative Analysis of Free and Bound Cholesterol and Cholesterol Ester Fatty Acids in Blood by Direct Mass Spectrometry. In: Frigerio, A. (eds) Recent Developments in Mass Spectrometry in Biochemistry and Medicine. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3991-5_25
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DOI: https://doi.org/10.1007/978-1-4613-3991-5_25
Publisher Name: Springer, Boston, MA
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