Abstract
The use of somatic cell hybrids formed between human and mouse cells led to the discovery that certain genes in human cells determine cell sensitivity or resistance to virus infections. The technique is based on the property of human–mouse somatic cell hybrids to lose most of the human chromosomes during cultivation under in vitro conditions. The human and mouse cells are fused by a virus (Sendai virus) or by the chemicals lysolecithin or polyethyleneglycol. The hybrid cells are propagated in culture, and the human and mouse chromosomes are defined by selective staining.
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© 1983 Martinus Nijhoff Publishers, The Hague
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Becker, Y., Hadar, J. (1983). Genes in Human Cells Determining Virus Susceptibility. In: Hadar, J. (eds) Molecular Virology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3906-9_4
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DOI: https://doi.org/10.1007/978-1-4613-3906-9_4
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