Summary
The poliovirus RNA-dependent RNA polymerase was isolated from cells infected with either the Sabin (LSC,2ab) or Mahoney strains of type 1 poliovirus. Both polymerases had similar levels of activity on all templates tested. Increasing the reaction temperature decreased the activity of both polymerases. No significant difference was found in the temperature sensitivity of the two enzymes. The size of the product RNA was measured by electrophoresis in CH3HgOH-agarose gels. Both enzymes were shown to synthesize full-sized product RNA and to have similar elongation rates at 30°C. Thus no significant difference was found between the in vitro activities of the two polymerases.
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© 1984 Martinus Nijhoff Publishing, Boston
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Flanegan, J.B., Clifford, C.A. (1984). Isolation and Characterization of the Viral RNA Dependent RNA Polymerases from Cells Infected with Either the Mahoney or Sabin Attenuated (LSc,2ab) Strains of Poliovirus Type 1. In: Kohn, A., Fuchs, P. (eds) Mechanisms of Viral Pathogenesis. Developments in Molecular Virology, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3894-9_22
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DOI: https://doi.org/10.1007/978-1-4613-3894-9_22
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