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Poxvirus DNA Replication

  • John A. Holowczak
Part of the Developments in Molecular Virology book series (DMVI, volume 2)

Summary

Vaccinia virus DNA replication occurs primarily in the cytoplasm of infected cells. Following adsorption, penetration and uncoating, at least two “nicks” are introduced into cell-associated, cross-linked, parental DNA molecules, which can be recovered from the cytoplasm of infected cells in the form of membrane-DNA complexes (p = 1.175 g/cni). Semiconservative, discontinuous viral DNA replication, requiring continuous protein synthesis, occurs initially in association with these membrane-parental DNA complexes and involves a partially circular or “rolling-circle” intermediate. The first product of DNA synthesis appears to be a concatemer in which unit-length genomes are held together, in head-to-head and tail-to-tail arrays. As the replication of these concatemers is completed, or nears completion, they associate together into larger, complex structures termed virosomes (p = 1.285 g/cm3). It is not clear if new rounds of DNA synthesis can be initiated in the virosomes or if partially replicated molecules are simply completed in these complexes. Unit lenght, but not yet cross-linked viral DNA molecules are excised from the virosomes and sealed, by a multi-enzyme system containing exonuclease, DNA polymerase and DNA ligase activities, present in the cytoplasm of infected cells. The mature, terminally cross-linked, 18ø kilobp, progeny genomes, are then encapsulated into developing vaccinia virions.

Keywords

Infected Cell Vaccinia Virus Cytoplasmic Fraction Inverted Terminal Repeat Parental Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Martinus Nijhoff Publishing, Boston 1983

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  • John A. Holowczak

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