Summary
A soluble, cytochrome P-450-dependent fatty acid hydroxylase-epoxidase complex from Bacillus megaterium ATCC 14581 can be induced more than 100-fold by the addition of phenobarbital or one of its analogs (hexobarbital) to the growth medium. These barbiturate inducers are apparently not substrates for the enzyme nor do they activate the monooxygenase in the cell-free system. The induction efficiency of both phenobarbital and hexobarbital can be significantly increased with respect to monooxygenase activity by autoclaving the inducer in the growth medium rather than by adding it to the medium after autoclaving. Turnover numbers of about 3 000 nmoles of substrate oxygenated per min per nmole of P-450 were obtained in crude cell-free preparations obtained from maximally induced cultures. Our data indicate that products formed by heating phenobarbital or hexobarbital in the growth medium are significantly better inducers of monooxygenase activity than are the unaltered drugs.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Miura, Y. and Fulco, A. J., 1974. J. Biol. Chem. 249: 1880–1888.
Miura, Y. and Fulco, A. J., 1975. Biochim. Biophys. Acta 388: 305–317.
Hare, R. S. and Fulco, A. J., 1975. Biochem. Biophys. Res. Commun. 65: 665–672.
Ho, P. P. and Fulco, A. J., 1976. Biochim. Biophys. Acta 431: 249–256.
Matson, R. S., Hare, R. S. and Fulco, A. J., 1977. Biochim. Biophys. Acta 487: 487–494.
Buchanan, J. F. and Fulco, A. J., 1978. Biochem. Biophys. Res. Commun. 85: 1254–1260.
Ruettinger, R. T. and Fulco, A. J., 1981. J. Biol. Chem. 256: 5228–5234.
Matson, R. S., Stein, R. A. and Fulco, A. J., 1980. Biochem. Biophys. Res. Commun. 97P: 955–961.
Matson, R. S. and Fulco, A. J., 1981. Biochem. Biophys. Res. Commun. 103: 531–535.
Berg, A. and Rafter, J., 1981. Biochem. J. 196: 781–786.
Gunsalus, I. C., Meeks, J. R., Lipscomb, J. D., DeBrunner, P. and Munck, E., 1974. In: Molecular Mechanisms of Oxygen Activation. (Hayaishi, O., ed.), pp. 559–613, New York: Academic Press.
Omura, T., Takemori, S., Suhara, K., Katagiri, M., Yoshida, Y. and Tagawa, K., 1978. In: Cytochrome P-450. (Sato, R. and Omura, T., eds.), pp. 138–208, Tokyo: Kodansha Ltd.
Salaun, J.-P., Benveniste, I., Reichhart, D. and Durst, F., 1981. Eur. J. Biochem. 119: 651–655.
Salaun, J.-P., Benveniste, I., Reichhart, D. and Durst, F., 1978. Eur. J. Biochem. 90: 155–159.
Narhi, L. O. and Fulco, A. J., 1982. J. Biol. Chem. 257: 2147–2150.
Nebert, D. W., Eisen, H. J., Negishi, M., Lang, M. A., Hjel- meland, L. M. and Okey, A. B., 1981. Ann. Rev. Pharmacol. Toxicol. 21: 431–462.
Okey, A. B., Bondy, G. P., Mason, M. E., Kahl, G. F., Eisen, H. J., Guenthner, T. M. and Nebert, D. W., 1979. J. Biol. Chem. 254: 11636–11648.
Adesnik, M., Bar-Nun, S., Maschio, F., Zunich, M., Lipp¬man, A. and Bard, E., 1981. J. Biol. Chem. 256: 10340–10345.
Poland, A., Mak, I. and Glover, E., 1981. Mol. Pharmacol. 20: 442–450.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1983 Martinus Nijhoff Publishers, Boston
About this chapter
Cite this chapter
Fulco, A.J., Kim, B.H., Matson, R.S., Narhi, L.O., Ruettinger, R.T. (1983). Nonsubstrate induction of a soluble bacterial cytochrome P-450 monooxygenase by phenobarbital and its analogs. In: Najjar, V.A. (eds) Enzyme Induction and Modulation. Developments in molecular and cellular biochemistry, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3879-6_9
Download citation
DOI: https://doi.org/10.1007/978-1-4613-3879-6_9
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-3881-9
Online ISBN: 978-1-4613-3879-6
eBook Packages: Springer Book Archive