Modulation of functional activities in cultured rat hepatocytes

  • Christiane Guguen-Guillouzo
  • André Guillouzo
Part of the Developments in molecular and cellular biochemistry book series (DMCB, volume 3)

Summary

Rat hepatocytes isolated by enzymatic dissociation of the liver must attach in order to survive for more than a few hours. In conventional culture conditions, they rapidly lose their highly differentiated functions, e.g. adult isozymic forms, enzyme response to specific hormones and cytochrome P-450-dependent monoox-ygenase activities. Incompletely differentiated cells such as perinatal and regenerating hepatocytes, can transiently exhibit a more differentiated state. Therefore, regulation of hepatic functions, particularly enzyme activities cannot be studied for more than a few days.

Hepatocyte survival rate and maintenance of specific functions are dependent on nutrient composition of the medium as well as the substrate. Complex matrices, particularly that derived from the connective liver biomatrix, appear to have an important favorable effect. However, regardless of culture conditions specific functions cannot be quantitatively maintained for more than several days.

Recent observations strongly suggest that such a problem may be overcome by mimicking in vivo specific cell-cell interactions. Thus when co-cultured with a liver epithelial cell line, probably derived from biliary ductular cells, adult hepatocytes remain able to synthesize high levels of albumin and to conjugate drugs. In these conditions, the cells secrete an abundant heterogenous extracellular material. The co-cultures can be maintained in a serum-free medium and specific liver functions can be altered experimentally. Such a model could be appropriate for studying long-term induction and modulation of liver enzyme activities under defined experimental conditions.

Keywords

Albumin Testosterone Pyruvate Pyrene Aniline 

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References

  1. 1.
    Greengard, O., 1977. Pediat. Res. 11: 669–676.PubMedCrossRefGoogle Scholar
  2. 2.
    Schwartz, R. I. and Bissell, M. J., 1977. Proc. Natl. Acad. Sci. U.S.A. 74: 4451–4457.Google Scholar
  3. 3.
    Bissell, D. M., 1981. Fed. Proc. 40: 2469–2473.PubMedGoogle Scholar
  4. 4.
    Watanabe, H., 1966. Exp. Cell Res. 42: 685–699.PubMedCrossRefGoogle Scholar
  5. 5.
    Alexander, R. W. and Grisham, J. W., 1970. Lab. Invest. 22: 50–62.PubMedGoogle Scholar
  6. 6.
    Guillouzo, A., Oudea, P., Le Guilly, Y., Oudea, M. C., Lenoir, P. and Bourel, M., 1972. Exp. Mol. Path. 16: 1–15.CrossRefGoogle Scholar
  7. 7.
    Guillouzo, A., Guguen, C., Lenoir, P. and Bourel, M., 1972. Compt. Rend. Acad. Sci. ( Paris ) 274: 484–487.Google Scholar
  8. 8.
    Le Guilly, Y., Simon, M., Lenoir, P. and Bourel, M., 1973. Gerontologia 19: 303–313.PubMedCrossRefGoogle Scholar
  9. 9.
    Kaighn, M. E. and Prince, A. M., 1971. Proc. Natl. Acad. Sci. U.S.A. 68: 2396–2400.PubMedCrossRefGoogle Scholar
  10. 10.
    Le Guilly, Y., Lenoir, P. and Bourel, M., 1973. Biomedi- cine 19: 361–365.Google Scholar
  11. 11.
    Williams, G. M. and Gunn, J. M., 1974. Exp. Cell Res. 69: 139–142.CrossRefGoogle Scholar
  12. 12.
    Morel-Chany, E., Guillouzo, C., Trincal, G. and Szajnert, M. F., 1978. Eur. J. Cancer 14: 1341–1352.PubMedGoogle Scholar
  13. 13.
    Gerschenson, L. E., Anderson, M., Molson, J. and Okiga- ki, T., 1970. Science 170: 859–861.PubMedCrossRefGoogle Scholar
  14. 14.
    Borek, C., 1972. Proc. Natl. Acad. Sci. U.S.A. 69: 956–959.PubMedCrossRefGoogle Scholar
  15. 15.
    Sirica, A. E. and Pitot, H. C., 1980. Pharmacol. Rev. 31: 205–228.Google Scholar
  16. 16.
    Grisham, J. W., 1980. Ann. N.Y. Acad. Sci. 349: 128–137.PubMedCrossRefGoogle Scholar
  17. 17.
    Pitot, H. C., Peraino, C., Morse, P. A. and Potter, V. R., 1964. Natl. Cancer Inst. Monogr. 13: 229–242.PubMedGoogle Scholar
  18. 18.
    Howard, R. B., Christensen, A. K., Gibbs, F. A. and Pesh, L. A., 1967. J. Cell Biol. 35: 675–684.PubMedCrossRefGoogle Scholar
  19. 19.
    Berry, M. N. and Friend, D. S., 1969. J. Cell Biol. 43: 506–520.PubMedCrossRefGoogle Scholar
  20. 20.
    Seglen, P. O., 1973. Exp. Cell Res. 76: 25–30.PubMedCrossRefGoogle Scholar
  21. 21.
    Le Cam, A., Guillouzo, A. and Freychet, P., 1976. Exp. Cell Res. 98: 383–395.Google Scholar
  22. 22.
    Guguen-Guillouzo, C., Campion, J. P., Brissot, P., Glaise, D., Launois, B., Bourel, M. and Guillouzo, A., 1982. Cell Biol. Intern. Rep. 6: 625–628.CrossRefGoogle Scholar
  23. 23.
    Bernaert, D., Wanson, J. C., Mosselmans, R., De Parmentier, F. and Drochmans, P., 1979. J. Cell Biol. 34: 159–174.Google Scholar
  24. 24.
    Le Rumeur, E., Beaumont, C., Guillouzo, C., Rissel, M., Bourel, M. and Guillouzo, A., 1981. Biochem. Biophys. Res. Commun. 101: 1038–1046.PubMedCrossRefGoogle Scholar
  25. 25.
    Leffert, H. L. and Paul, D., 1972. J. Cell Biol. 52: 559–568.PubMedCrossRefGoogle Scholar
  26. 26.
    Guguen-Guillouzo, C., Marie, J., Cottreau, D., Pasdeloup, N. and Kahn, A., 1980. Biochem. Biophys. Res. Commun. 93: 528–534.PubMedCrossRefGoogle Scholar
  27. 27.
    Kato, S., Aoyama, K., Nakamura, T. and Ichihara, A., 1979. J. Biochem. 86: 1419–1425.PubMedGoogle Scholar
  28. 28.
    Groothuis, G. M. M., Hulstaert, C. E., Kalicharan, D. and Hardonk, M. J., 1981. Eur. J. Cell Biol. 26: 43–51.PubMedGoogle Scholar
  29. 29.
    Allen, C. M., Hockin, L.J. and Paine, A. J., 1981. Biochem. Pharmacol. 30: 2739–2742.PubMedCrossRefGoogle Scholar
  30. 30.
    Tanaka, K., Sato, M., Tomita, Y. and Ichihara, A., 1978. J. Biochem. 84: 937–946.PubMedGoogle Scholar
  31. 31.
    Takeda, Y., Ichihara, A., Tanioka, H. and Inoue, H., 1964. J. Biol. Chem. 239: 3590–3596.PubMedGoogle Scholar
  32. 32.
    Jeejeebhoy, K.N. and Phillips, M. J., 1976. Gastroente¬rology 71: 1086–1096.Google Scholar
  33. 33.
    Jeejeebhoy, K. N., Phillips, M. J., Ho, J. and Bruce-Ro¬bertson, A., 1980. Ann. N.Y. Acad. Sci. 349: 18–27.PubMedCrossRefGoogle Scholar
  34. 34.
    Seglen, P. O., 1976. Meth. Cell Biol. 13: 29–83.CrossRefGoogle Scholar
  35. 35.
    Dalet, C., Fehlmann, M. and Debey, P., 1982. Anal. Bio¬chem. 122: 119–123.CrossRefGoogle Scholar
  36. 36.
    Guguen-Guillouzo, C., Tichonicky, L., Szajnert, M. F., and Kruh, J., 1980. In Vitro 16: 1–10.PubMedCrossRefGoogle Scholar
  37. 37.
    Bissell, D. M., Hammaker, L. E., Walker, P. R. and Pot¬ter, V. R., 1973. J. Cell Biol. 59: 722–734.PubMedCrossRefGoogle Scholar
  38. 38.
    Chapman, G. S., Jones, A. L., Meyer, U. A. and Bissell, D. M., 1973. J. Cell Biol. 59: 735–745.PubMedCrossRefGoogle Scholar
  39. 39.
    Guguen, C., Guillouzo, A., Boisnard, M., Le Cam, A. and Bourel, M., 1975. Biol. Gastroenterol. 8: 223–231.Google Scholar
  40. 40.
    Wanson, J. C., Drochmans, P., Mosselmans, R. and Ron- veaux, M. F., 1977. J. Cell Biol. 74: 838–877.CrossRefGoogle Scholar
  41. 41.
    Guguen-Guillouzo, C., Tichonicky, L., Szajnert, M. F., Schapira, F. and Kruh, J., 1978. Biol. Cell 31: 225–234.Google Scholar
  42. 42.
    Bonney, R.J., Becker, J. E., Walker, P. R. and Potter, V. R., 1974. In Vitro 9: 399–413.CrossRefGoogle Scholar
  43. 43.
    Spence, J. T., Haars, L., Edwards, A., Bosch, A. and Pitot, H. C., 1980. Ann. N.Y. Acad. Sci. 349: 99–110.PubMedCrossRefGoogle Scholar
  44. 44.
    Pariza, M. W., Yager, J. D., Jr., Goldfarb, S., Gurr, J. A., Yanachi, S., Grossman, S. H., Becker, J. E., Barber, T. A. and Potter, V. R., 1975. Gene expression and carcinogenesis in cultured liver (Gerschenson, L. E. and Thompson, E. B., eds.), pp. 137–167, Academic Press, New York.Google Scholar
  45. 45.
    Lin, R. C. and Snodgrass, P. J., 1975. Biochem. Biophys. Res. Commun. 64: 725-734.Google Scholar
  46. 46.
    Schapira, F., 1981. Cur. Top. Biol. Med. Res. 5: 27–75.Google Scholar
  47. 47.
    Guguen, C., Gregori, C. and Schapira, F., 1975. Biochimie 57: 1065–1071.PubMedCrossRefGoogle Scholar
  48. 48.
    Sirica, A. E., Richards, W., Tsukada, C., Sattler, C. and Pitot, H.C., 1979. Proc. Natl. Acad. Sci. U.S.A. 76: 283–287.PubMedCrossRefGoogle Scholar
  49. 49.
    Feliu, J. E., Coloma, J., Gomez-Lechon, M. J., Garcia, M. D. and Baguena, J., 1982. Mol. Cell Biochem. 45: 73–81.PubMedCrossRefGoogle Scholar
  50. 50.
    Gurr, J. A. and Potter, V. R., 1980. Exp. Cell Res. 126: 237–248.PubMedCrossRefGoogle Scholar
  51. 51.
    Savage, C. R. and Bonney, R. J., 1978. Exp. Cell Res. 114: 307–315.PubMedCrossRefGoogle Scholar
  52. 52.
    Gurr, J. A. and Potter, V. R., 1980. Ann. N.Y. Acad. Sci. 349: 57–66.PubMedCrossRefGoogle Scholar
  53. 53.
    Guzelian, P. S., Bissell, D. M. and Meyer, U. A., 1977. Gastroenterology 72: 1232–1239.PubMedGoogle Scholar
  54. 54.
    Bissell, D. M. and Guzelian, P. S., 1979. Arch. Biochem. Biophys. 192: 569–576.PubMedCrossRefGoogle Scholar
  55. 55.
    Michalopoulos, G., Russell, G. and Biles, C., 1979. In Vitro 15: 796–806.PubMedCrossRefGoogle Scholar
  56. 56.
    Paine, A. J. and Legg, R. F., 1978. Biochem. Biophys. Res. Commun. 81: 672–679.PubMedCrossRefGoogle Scholar
  57. 57.
    Bissell, D. M. and Guzelian, P. S., 1975. Gene expression and carcinogenesis in cultured liver (Gerschenson, L. E. and Thompson, E. B., eds.), pp. 119–136, Academic Press, New York.Google Scholar
  58. 58.
    Schmeltz, I., Tosk, J. and Williams, G. M., 1978. Cancer Lett. 5: 81–89.PubMedCrossRefGoogle Scholar
  59. 59.
    Bissell, D. M., Deal, D. R. and Hammaker, L. E., 1975. Gastroenterology 69: 809.Google Scholar
  60. 60.
    Guillouzo, A., Beaumont, C., Le Rumeur, E., Rissel, M., Latinier, M. F., Guguen-Guillouzo, C. and Bourel, M., 1982. Biol. Cell 43: 163–172.Google Scholar
  61. 61.
    Haars, L. and Pitot, H. C., 1979. J. Biol. Chem. 254: 9401–9407.PubMedGoogle Scholar
  62. 62.
    May, C., Popowski, A., Mosselmans, R. and Wanson, J. C., 1979. Biol. Cell 35: 66a.Google Scholar
  63. 63.
    Le Rumeur, E., Beaumont, C., Guguen-Guillouzo, C. and Guillouzo, A., 1982. Biol. Cell 45: 71.Google Scholar
  64. 64.
    Wanson, J. C., Bernaert, D., May, C., Deschuyteneer, M. and Prieels, J. P., 1980. Ann. N.Y. Acad. Sci. 349: 413–415.Google Scholar
  65. 65.
    Hata, R., Ninomiya, Y., Nagai, Y. and Tsukada, Y., 1979. Proc. Japan Acad. 55: 531–536.CrossRefGoogle Scholar
  66. 66.
    Tseng, S. C. G., Lee, P. C., Ells, P. F., Bissell, D. M., Smuckler, E. A. and Stern, R., 1982. HepatolGoogle Scholar
  67. 67.
    Guzelian, P. S. and Diegelmann, R. F., 1979. Exp. Cell Res. 123: 269–279.PubMedCrossRefGoogle Scholar
  68. 68.
    Takeuchi, T. and Prokkop, D. J., 1969. Gastroenterology 56: 744–000.Google Scholar
  69. 69.
    Diegelmann, R. F., Cohen, I. K. and Guzelian, P. S., 1980. Biochem. Biophys. Res. Commun. 97: 819–826.PubMedCrossRefGoogle Scholar
  70. 70.
    Tarentino, A. L. and Galivan, J., 1980. In Vitro 16: 833–846.PubMedCrossRefGoogle Scholar
  71. 71.
    Seglen, P. O. and Solheim, A. E., 1978. Biochim. Biophys. Acta. 520: 630–641.PubMedGoogle Scholar
  72. 72.
    Seglen, P. O., Solheim, A. E., Grinde, B., Gordon, P. B., Schwarze, P. E., Gjessing, R. and Poli, A., 1980. Ann. N.Y. Acad. Sci. 349: 1–17.PubMedCrossRefGoogle Scholar
  73. 73.
    Leffert, H., Moran, T., Sell, S., Skelly, H., Ibsen, K., Mueller, M. and Arias, I., 1978. Proc. Natl. Acad. Sci. U.S.A. 75: 1834–1838.PubMedCrossRefGoogle Scholar
  74. 74.
    Edwards, A. M., 1982. Cancer Res. 42: 1107–1115.PubMedGoogle Scholar
  75. 75.
    Kruh, J., Courtois, Y., Dastugue, B., Defer, N., Gibson, K., Kamiyama, M. and Tichonicky, L., 1975. Regulation of growth and differentiated functions in eukaryote cells (Talwar, G. P., ed.), pp. 139 - 154, Raven Press, New York.Google Scholar
  76. 76.
    Althaus, F. R., Lawrence, S. D., Sattler, G. L. and Pitot, H. C., 1982. J. Biol. Chem. 257: 5528–5535.PubMedGoogle Scholar
  77. 77.
    Plas, C., 1969. Comp. Rend. Acad. Sci. ( Paris ) 268: 143–146.Google Scholar
  78. 78.
    Armato, U., Andreis, P. G., Draghi, E. and Mengato, L., 1978. In Vitro 14: 479–484.PubMedCrossRefGoogle Scholar
  79. 79.
    Rupriak, H. T. and Paul, D., 1978. Biochim. Biophys. Acta 543: 10–15.Google Scholar
  80. 80.
    Acosta, D., Anuforo, D. C. and Smith, R. V., 1978. In Vitro 14: 428–436.PubMedCrossRefGoogle Scholar
  81. 81.
    Armato, U., Andreis, P. G., Draghi, E., Negri, L., Mengato, L. and Neri, G., 1978. In Vitro 14: 838–848.PubMedCrossRefGoogle Scholar
  82. 82.
    Armato, U., Draghi, E. and Andreis, P. G., 1975. Cell Differ. 4: 147–153.PubMedCrossRefGoogle Scholar
  83. 83.
    Guillouzo, A., Feldmann, G., Boisnard, M., Sapin, C. and Benhamou, J. P., 1975. Exp. Cell Res. 96: 239–246.PubMedCrossRefGoogle Scholar
  84. 84.
    Sell, S., Skelly, H., Leffert, H. L., Muller-Eberhard, H. and Kida, S., 1975. Ann. N.Y. Acad. Sci. 259: 45–58.PubMedCrossRefGoogle Scholar
  85. 85.
    Plas, C. and Nunez, J., 1975. J. Biol. Chem. 250: 5304–5311.PubMedGoogle Scholar
  86. 86.
    Plas, C., Menuelle, P., Moncany, M. L. J. and Fulchigno- ni-Lataud, C., 1979. Diabetes 28: 705–112.PubMedGoogle Scholar
  87. 87.
    Sereni, F., Kenney, F. T. and Kretchmer, N., 1959. J. Biol. Chem. 234: 609–612.PubMedGoogle Scholar
  88. 88.
    Cake, M. H., Yeoh, G. C. T. and Oliver, I. T., 1981. Biochem. J. 198: 301–307.PubMedGoogle Scholar
  89. 89.
    Yeoh, G. C. T., Bennett, F. A. and Oliver, I. T., 1979. Biochem. J. 180: 153–160.PubMedGoogle Scholar
  90. 90.
    Yeoh, G. C. T., Arbuckle, T. and Oliver, I. T., 1979. Biochem. J. 180: 545–549.PubMedGoogle Scholar
  91. 91.
    Leakey, J. E. A. and Fouts, J. R., 1979. Biochem. J. 182: 233–235.PubMedGoogle Scholar
  92. 92.
    Kremers, P., Goujon, F., De Graeve, J., Van Cantfort, J. and Gielen, J. E., 1981. Eur. J. Biochem. 116: 67–72.PubMedCrossRefGoogle Scholar
  93. 93.
    Nakamura, T., Aoyama, K. and Ichihara, A., 1979. Biochem. Biophys. Res. Commun. 91: 515–520.PubMedCrossRefGoogle Scholar
  94. 94.
    Wakelam, M. J. O. and Walker, D. G., 1980. FEBS Lett. 111: 115–119.PubMedCrossRefGoogle Scholar
  95. 95.
    Nakamura, T., Aoyama, K., Tomomura, A. and Ichihara, A., 1981. Biochim. Biophys. Acta 678: 91–79.PubMedGoogle Scholar
  96. 96.
    Greegard, O., 1969. Biochem. J. 115: 19–24.Google Scholar
  97. 97.
    Mandelli, M., Piceni-Sereni, L. and Sereni, F., 1973. Bio¬chim. Biophys. Acta 309: 515–520.Google Scholar
  98. 98.
    Dodek, P., Kirby, L., Frohlich, J., Hahn, P. and Ho-Yuen, B., 1975. Proc. Soc. Exp. Biol. Med. 150: 7–10.PubMedGoogle Scholar
  99. 99.
    Dyson, R. D., Cardenas, J. M., Richards, T. C. and Garnett, M. E., 1977. Biochem. Biophys. Acta 481: 115–126.PubMedGoogle Scholar
  100. 100.
    Guguen-Guillouzo, C. and Hatzfeld, A., 1979. Protides in Biological Fluids (Peters, H. ed.), Vol. 27, pp. 157–160, Pergamon Press, Oxford.Google Scholar
  101. 101.
    Croizat, B., Granelli-Piperno, A. and Gros, F., 1974. Biochimie 56: 305–313.PubMedCrossRefGoogle Scholar
  102. 102.
    Guguen-Guillouzo, C., Tichonicky, L. and Kruh, J., 1979. Eur. J. Biochem. 178: 89–95.Google Scholar
  103. 103.
    Guguen-Guillouzo, C., Tichonicky, L., Glaise, D. and Kruh, J., 1982. Biol. Cell 44: 101–110.Google Scholar
  104. 104.
    Marceau, N., Noel, M. and Deschenes, J., 1982. In Vitro 18: 1–11.PubMedCrossRefGoogle Scholar
  105. 105.
    Richman, R. A., Claus, T. H., Pilkis, S. J. and Friedman, D. L., 1976. Proc. Natl. Acad. Sci. U.S.A. 73: 3589–3593.PubMedCrossRefGoogle Scholar
  106. 106.
    Bonney, R. J., Hopkins, M. A., Walker, P. R. and Potter, V. R., 1973. Biochem. J. 136: 947–953.PubMedGoogle Scholar
  107. 107.
    Garnett, M. E., Dyson, R. D. and Dost, F. N., 1973. J. Biol. Chem. 249: 5222–5226.Google Scholar
  108. 108.
    Walker, P. R. and Potter, V. R., 1972. Adv. Enz. Regul. 10: 339–364.CrossRefGoogle Scholar
  109. 109.
    Guguen-Guillouzo, C., Szajnert, M. F., Glaise, D., Grego- ri, C. and Schapira, F., 1981. In Vitro 17: 369–377.PubMedCrossRefGoogle Scholar
  110. 110.
    Weber, A., Hatzfeld, A., Guillouzo, A. and Schapira, F., 1979. Biochem. Biophys. Res. Commun. 86: 6–13.PubMedCrossRefGoogle Scholar
  111. 111.
    Scherer, E. and Emmelot, E., 1975. Eur. J. Cancer 11: 145–153.PubMedGoogle Scholar
  112. 112.
    Pitot, H. C., Barsness, L., Goldworthy, T. and Kitagawa, T., 1978. Nature 271: 456–458.PubMedCrossRefGoogle Scholar
  113. 113.
    Solt, D. and Farber, E., 1976. Nature 263: 701–703.CrossRefGoogle Scholar
  114. 114.
    Judah, D. J., Legg, R. F. and Neal, G. E., 1977. Nature 265, 343–345.PubMedCrossRefGoogle Scholar
  115. 115.
    Wanson, J. C., Bernaert, D., Penasse, W., Mosselmans, R. and Bannasch, P., 1980. Cancer Res. 40: 459–471.PubMedGoogle Scholar
  116. 116.
    Williams, G. M., 1976. Cancer Res. 36: 2540–2543.PubMedGoogle Scholar
  117. 117.
    Laishes, B. A., Ogawa, K., Roberts, E. and Farber, E., 1978. J. Natl. Cancer Inst. 60: 1009–1016.PubMedGoogle Scholar
  118. 118.
    Laishes, B. A., Roberts, E. and Farber, E., 1978. Int. J. Cancer 21: 186–193.PubMedCrossRefGoogle Scholar
  119. 119.
    Iype, P. T., Tomaszewski, J. E. and Dyple, A., 1979. Cancer Res. 39: 4925–4929.PubMedGoogle Scholar
  120. 120.
    Sato, G. and Reid, L., 1978. Biochemistry and mode of action of hormones II (Rickenberg, H. V., ed.), Vol. 20, pp. 219 - 251, University Park Press, Baltimore.Google Scholar
  121. 121.
    Gatmaitan, Z. and Reid, L., 1980. Gastroenterology 79: 1019.Google Scholar
  122. 122.
    Bernaert, D., Wanson, J. C., Drochmans, P. and Popows- ki, A., 1977. J. Cell Biol. 74: 878–900.PubMedCrossRefGoogle Scholar
  123. 123.
    Laishes, B. A. and Williams, G. M., 1976. In Vitro 12: 521–532.PubMedCrossRefGoogle Scholar
  124. 124.
    Michalopoulos, G. and Pitot, H. C., 1975. Exp. Cell Res. 94: 70–78.PubMedCrossRefGoogle Scholar
  125. 125.
    Blackard, W. G., Guzelian, P. S. and Small, M. E., 1978. Endocrinology 103: 548–553.PubMedCrossRefGoogle Scholar
  126. 126.
    Koontz, J. W. and Iwahashi, M., 1981. Science 211: 947–949.PubMedCrossRefGoogle Scholar
  127. 127.
    Crane, L. J. and Miller, D. L., 1977. J. Cell Biol. 72: 11–25.PubMedCrossRefGoogle Scholar
  128. 128.
    Wagle, S. R., Ingerbretsen, W. R. and Sampson, L., 1973. Biochem. Biophys. Res. Commun. 53: 937–943.PubMedCrossRefGoogle Scholar
  129. 129.
    Ichihara, A., Nakamura, T., Tanaka, K., Tomita, Y., Aoyama, K., Kato, S. and Shinno, H., 1980. Ann. N.Y. Acad. Sci. 349: 77–84.PubMedCrossRefGoogle Scholar
  130. 130.
    Nakamura, T., Yoshimoto, K., Aoyama, K. and Ichihara, A., 1982. J. Biochem. 91: 681–693.PubMedGoogle Scholar
  131. 131.
    Leffert, H. L., 1974. J. Cell Biol. 62: 792–801.PubMedCrossRefGoogle Scholar
  132. 132.
    Leffert, H. L., Moran, T., Boorstein, R. and Koch, K. S., 1977. Nature 267: 58–61.PubMedCrossRefGoogle Scholar
  133. 133.
    Katz, N. R. and Ick, M., 1981. Biochem. Biophys. Res. Commun. 100: 703–709.PubMedCrossRefGoogle Scholar
  134. 134.
    Spence, J. T., Merril, M. J. and Pitot, H. C., 1981. J. Biol. Chem. 256: 1598–1603.PubMedGoogle Scholar
  135. 135.
    Tomita, Y., Nakamura, T. and Ichihara, A., 1981. Exp. Cell Res. 135: 363–371.PubMedCrossRefGoogle Scholar
  136. 136.
    Laishes, B. A. and Williams, G. M., 1976. In Vitro 12: 821–832.PubMedCrossRefGoogle Scholar
  137. 137.
    Laishes, B. A., Roberts, E. and Burrowes, C., 1976. Biochem. Biophys. Res. Commun. 72: 462–471.PubMedCrossRefGoogle Scholar
  138. 138.
    Williams, G. M., Bermudez, E., San, R. H. C., Goldblatt, P. J. and Laspia, M. F., 1978. In Vitro 14: 824–837.PubMedCrossRefGoogle Scholar
  139. 139.
    Coloma, J., Gomez-Lechon, M. J., Garcia, M. D., Feliu, J. E. and Baguena, J., 1981. Experientia 37: 941–942.PubMedCrossRefGoogle Scholar
  140. 140.
    Billat, C., Plas, C. and Jacquot, R., 1972. Compt. Rend. Acad. Sci. ( Paris ) 274: 2338–2340.Google Scholar
  141. 141.
    Marceau, N., Goyette, R., Valet, J. P. and Deschenes, J.,1980. Exp. Cell Res. 125: 497–502.PubMedCrossRefGoogle Scholar
  142. 142.
    Failla, M. L. and Cousins, R. J., 1978. Biochim. Biophys. Acta 543: 293–304.PubMedGoogle Scholar
  143. 143.
    Schudt, C., 1979. Eur. J. Biochem. 98: 77–82.PubMedCrossRefGoogle Scholar
  144. 144.
    Schudt, C., 1980. Biochim. Biophys. Acta 629: 499–509.PubMedGoogle Scholar
  145. 145.
    Plas, C. and Nunez, J., 1976. J. Biol. Chem. 251:1431 –1437.PubMedGoogle Scholar
  146. 146.
    Katz, N. R., Nauck, M. A. and Wilson, P. T., 1979. Biochem. Biophys. Res. Commun. 88: 23–29.PubMedCrossRefGoogle Scholar
  147. 147.
    Gebhardt, R. and Mecke, D., 1979. Eur. J. Biochem. 97: 29–35.PubMedCrossRefGoogle Scholar
  148. 148.
    Stump, D. J. and Kletzien, R. F., 1981. J. Cell Physiol. 107: 11–19.CrossRefGoogle Scholar
  149. 149.
    Wilson, E. J. and McMurray, C., 1980. Biochem. Biophys. Res. Commun. 93: 179–185.PubMedCrossRefGoogle Scholar
  150. 150.
    Friedman, D. L., Claus, T. H., Pilkis, S. J. and Pine, G. E.,1981.Exp. Cell Res. 135: 283–290.PubMedCrossRefGoogle Scholar
  151. 151.
    Paine, A. J., Williams, L.J. and Legg, R. F., 1979. Life Sci. 24: 2185–2192.PubMedCrossRefGoogle Scholar
  152. 152.
    Michalopoulos, G., Sattler, C. A., Sattler, G. L. and Pitot, H. C., 1976. Science 193: 907–909.PubMedCrossRefGoogle Scholar
  153. 153.
    Decad, G. M., Hsieh, D. P. H. and Byard, J. L., 1977. Biochem. Biophys. Res. Commun. 78: 279–287.PubMedCrossRefGoogle Scholar
  154. 154.
    Dickins, M. and Peterson, R. E., 1980. Biochem. Pharmacol. 29: 1231–1238.PubMedCrossRefGoogle Scholar
  155. 155.
    Guzelian, P. S. and Bissell, D. M., 1976. J. Biol. Chem. 251: 4421–4427.PubMedGoogle Scholar
  156. 156.
    Paine, A. J., Hockin, L. J. and Legg, R. F., 1979. Biochem. J. 184: 461–463.PubMedGoogle Scholar
  157. 157.
    Fahl, W. E., Michalopoulos, G., Sattler, G. L., Jefcoate, C. R. and Pitot, H. C., 1979. Arch. Biochem. Biophys. 192: 61–72.PubMedCrossRefGoogle Scholar
  158. 158.
    Stenberg, A. and Gustafsson, J. A., 1978. Biochim. Biophys. Acta 540: 402–407.Google Scholar
  159. 159.
    Fry, J. R., Wiebkin, P. and Bridges, J. W., 1980. Biochem. Pharmacol. 29: 577–581.PubMedCrossRefGoogle Scholar
  160. 160.
    Toftgard, R., Nilsen, O. G., Ingelman-Sundberg, M. and Gustafsson, J. A., 1980. Acta Pharmacol. Toxicol. 46: 353–361.CrossRefGoogle Scholar
  161. 161.
    Elshourbagy, N. A., Barwick, J. L. and Guzelian, P. S., 1981. J. Biol. Chem. 256: 6060–6068.PubMedGoogle Scholar
  162. 162.
    Newman, S. L., Barwick, J. L., Elshourbagy, N. A. and Guzelian, P. S., 1982. Biochem. J. 204: 281–220.PubMedGoogle Scholar
  163. 163.
    Newman, S. and Guzelian, P. S., 1982. Proc. Natl. Acad. Sci. U.S.A. 79: 2922–2926.PubMedCrossRefGoogle Scholar
  164. 164.
    Bissell, D. M. and Guzelian, P. S., 1980. Ann. N.Y. Acad. Sci. 349: 85–98.PubMedCrossRefGoogle Scholar
  165. 165.
    Reid, L., Morrow, B., Jubinsky, P., Schwartz, E. and Gatmaitan, Z., 1981. Ann. N.Y. Acad. Sci. 364: 354–370.Google Scholar
  166. 166.
    Guguen, C., Guillouzo, A., Boisnard, M., Le Cam, A. and Bourel, M., 1975. Digestion 75: 171.Google Scholar
  167. 167.
    Hirsiger, H., Gautschi, S. R. and Schindler, R., 1976. Exp. Cell Res. 100: 356–364.PubMedCrossRefGoogle Scholar
  168. 168.
    Ponce, P., Cordero, J. and Rojkind, M., 1981. Hepatology 1: 204–210.PubMedCrossRefGoogle Scholar
  169. 169.
    Sattler, C. A., Michalopoulos, G., Sattler, G. L. and Pitot, H. C., 1978. Cancer Res. 38: 1539–1549.PubMedGoogle Scholar
  170. 170.
    Seglen, P. O. and Fossa, J., 1978. Exp. Cell Res. 116: 199–206.PubMedCrossRefGoogle Scholar
  171. 171.
    Rubin, K., Johansson, S., Pettersson, I., Ocklind, O., Obrink, B. and Hook, M., 1979. Biochem. Biophys. Res. Commun. 91: 86–94.Google Scholar
  172. 172.
    Rubin, K., Hook, M., Obrink, B. and Timpl, R., 1981. Cell 24: 463–470.PubMedCrossRefGoogle Scholar
  173. 173.
    Rojkind, M., Mackensen, S., Giambrone, M. A., Ponce, P. and Reid, L., 1980. J. Cell. Biol. 87: 255–283.Google Scholar
  174. 174.
    Gospodarowicz, D., Moran, J., Braun, D. and Birdwell,C. A., 1976. Proc. Natl. Acad. Sci. U.S.A. 73: 4120–4124.Google Scholar
  175. 175.
    Gospodarowicz, D., Greenburg, G., Vlodarsky, I., Alvaro, J. and Johnson, L. K., 1979. Exp. Eye Res. 29: 485–509.PubMedCrossRefGoogle Scholar
  176. 176.
    Tseng, S. C. G., Savion, N., Gospodarowicz, D. and Stern, R., 1981. J. Biol. Chem. 256: 3361–3365.Google Scholar
  177. 177.
    Guguen-Guillouzo, C., Seignoux, D., Courtois, Y., Bris- sot, P., Marceau, N., Glaise, D. and Guillouzo, A., 1982. Biol. Cell 46: 11–20.Google Scholar
  178. 178.
    Gospodarowicz, D., Greenburg, G. and Birdwell, C. R., 1978. Cancer Res. 38: 4155–4171.PubMedGoogle Scholar
  179. 179.
    Cervera, M., Dreyfuss, G. and Penman, S., 1981. Cell 23: 113–120.PubMedCrossRefGoogle Scholar
  180. 180.
    Emerman, J. T. and Pitelka, D. R., 1977. In Vitro 13: 316–328.PubMedCrossRefGoogle Scholar
  181. 181.
    Albanese, J., Kuhlenschmidt, M., Schmell, E., Slife, C. W. and Roseman, S., 1982. J. Biol. Chem. 257: 3165–3170.PubMedGoogle Scholar
  182. 182.
    Wanson, J. C., Mosselmans, R., Brouwer, A. and Knook, D. L., 1979. Biol. Cell 36: 7–16.Google Scholar
  183. 183.
    Le Douarin, N. M., 1975. Med. Biol. 53: 427–455.PubMedGoogle Scholar
  184. 184.
    Grobstein, C., 1975. Extracellular matrix influences on gene expression (Slavkin, M. C., Grenlich, R. C., Brinkley, B. R. and Porter, K. R., eds.), pp. 9–16, Academic Press, New York.Google Scholar
  185. 185.
    Rojkind, M., 1982. The Liver; Biology and Pathobiology (Arias, I., Popper, H., Schachter, D. and Schafritz, D. A., eds.), pp. 537–548, Raven Press, New York.Google Scholar
  186. 186.
    Wilson, J. W., Groat, C. S. and Leduc, E. H., 1963. Ann. N.Y. Acad. Sci. Ill: 8–22.Google Scholar
  187. 187.
    Guguen-Guillouzo, C., 1982. Biol. Cell 45: 17.Google Scholar
  188. 188.
    Guguen-Guillouzo, C., Clement, B., Baffet, G., Beaumont, C., Morel-Chany, E., Glaise, D. and Guillouzo, A., 1983. Exp. Cell Res. 143: 47–54.PubMedCrossRefGoogle Scholar
  189. 189.
    Guguen-Guillouzo, C., Lescoat, G., Baffet, G., Le Rumeur, E., Clement, B., Glaise, D. and Guillouzo, A., 1983. Proc. First Europ. Conf. on Serum-free Cell Culture (Fischer, G., ed.), Springer Verlag, Heidelberg, in press.Google Scholar
  190. 190.
    Baffet, G., Clement, B., Glaise, D., Guillouzo, A. and Guguen-Guillouzo, C., 1982. Biochem. Biophys. Res. Commun. 109: 507–512.PubMedCrossRefGoogle Scholar
  191. 188.
    Guguen-Guillouzo, C., Baffet, G., Clement, B., Begue, J. M., Glaise, D. and Guillouzo, A., 1983. Isolation charac¬terization and use of Hepatocytes (Harris, R. A. and Cor¬nell, N. W., eds.), Elsevier, in press.Google Scholar
  192. 189.
    Begue, J. M., Le Bigot, J. F., Guguen-Guillouzo, C., Kie- chel, J. R. and Guillouzo, A., 1983. Biochem. Pharmacol., in press.Google Scholar

Copyright information

© Martinus Nijhoff Publishers, Boston 1983

Authors and Affiliations

  • Christiane Guguen-Guillouzo
    • 1
  • André Guillouzo
    • 1
  1. 1.Unité de Recherches Hépatologiques U 49 de l’INSERMHôpital de PontchaillouRennes CedexFrance

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