Skip to main content
SpringerLink
Log in

Induction of fatty acid synthetase and acetyl-CoA carboxylase by isolated rat liver cells

  • Chapter
Enzyme Induction and Modulation

Part of the book series: Developments in molecular and cellular biochemistry ((DMCB,volume 3))

  • 72 Accesses

Summary

Current studies on the synthesis of long-chain fatty acids by isolated rat liver cells are largely concerned with the regulation of the activity of previously existing acetyl-CoA carboxylase and fatty acid synthetase, and with the regulation of the quantity of these enzymes. These studies have required the development of methods for obtaining high yields of viable hepatocytes that respond to hormonal treatment. Such methods have been developed over the past 10–15 years through the efforts of several laboratories. These studies have also required the development of a method to determine whether a change in the activity of an enzyme is due to a modification of preexisting enzyme or to a change in quantity of that enzyme. The most satisfactory method to use for such studies is immunotitration of enzyme activity.

In recent years studies on the regulation of acetyl-CoA carboxylase have largely centered upon the effect of phosphorylation-dephosphorylation on the activity of this enzyme and whether glucagon inhibits the activity of this enzyme through this process. Much data from a number of laboratories have suggested that glucagon regulates the activity of this enzyme through phosphorylation-dephosphorylation. However, several of these studies involved the use of crude systems in which competing enzymes and substrates that can significantly interfere with acetyl-CoA carboxylase activity measurements were still present. Hence, a confirmation of these studies needs to be carried out under conditions in which the effects of competing enzymes and substrates are eliminated.

Studies on changes in quantity of acetyl-CoA carboxylase and latty acid synthetase have shown that these enzymes are induced by the fasting and refeeding of animals. They have also shown that insulin stimulates (10- to 30-fold) the induction of these enzymes. This induction appears to be due to a change in the quantity of translatable mRNA which may, in turn, be due to a change in the rate of transcription of the genes coding for these enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Howard, R. B., Christensen, A. K., Gibbs, F. A. and Pesch, L. A., 1967. J. Cell Biol. 35, 675–684.

    Article  PubMed  CAS  Google Scholar 

  2. Berry, M. N. and Friend, D. S., 1969. J. Cell Biol. 43, 506–520.

    Article  PubMed  CAS  Google Scholar 

  3. Ingebretsen, W. R., Jr. and Wagle, S. R. 1972. Biochem. Biophys. Res. Commun. 47, 403–410.

    Article  PubMed  CAS  Google Scholar 

  4. Zahlten, R. N. and Stratman, F. W. 1974. Arch. Biochem. Biophys. 163, 600–608.

    Article  PubMed  CAS  Google Scholar 

  5. Seglen, P. O. 1976. Methods Cell Biol. 13, 29–83.

    Article  PubMed  CAS  Google Scholar 

  6. Wagle, S. R. and Ingebretsen, W. R., Jr. 1975. Methods Enzymol. 35, 579–594.

    Article  PubMed  CAS  Google Scholar 

  7. Seglen, P. O. 1973. FEBS Lett. 30, 25–28.

    Article  PubMed  CAS  Google Scholar 

  8. Akpan, J. O., Gardner, R. and Wagle, S. R. 1974. Biochem. Biophys. Res. Commun. 61, 222–228.

    Article  PubMed  CAS  Google Scholar 

  9. Witters, L. A., Alberico, L. and Avruch, J. 1976. Biochem. Biophys. Res. Commun. 69, 997–1003.

    Article  PubMed  CAS  Google Scholar 

  10. Bissell, D. M., Hammaker, L. E. and Meyer, U. S. 1973. J. Cell Biol. 59, 722–734.

    Article  PubMed  CAS  Google Scholar 

  11. Bonney, R. J., Becker, J. E., Walker, P. R. and Potter, V. R. 1974. In vitro 9, 399–413.

    Article  CAS  Google Scholar 

  12. Burton, D. N., Collins, J. M. and Porter, J. W. 1969. J. Biol. Chem. 244, 1076–1077.

    PubMed  CAS  Google Scholar 

  13. Howard, R. B. and Pesch, L. A. 1968. J. Biol. Chem. 243, 3105–3109.

    PubMed  CAS  Google Scholar 

  14. Cohen, P. P. 1957. in ManometricTechniques(Umbreit, W. W., Burris, R. H. and Stauffer, J. F., eds.) p. 149, Burgess Publishing Company, Minneapolis.

    Google Scholar 

  15. Siekevitz, P. 1952. J. Biol. Chem. 195, 549–565.

    Google Scholar 

  16. Lakshmanan, M. R., Nepokroeff, C. M., Kim, M. and Porter, J. W. 1975. Arch. Biochem. Biophys. 169, 737–745.

    Google Scholar 

  17. Geelen, M. J. H. and Gibson, D. M. 1976. In: Use of Isolated Liver Cells and Kidney Tubules in Metabolic Studies (Tager, J. M., Soling, H. D. and Williamson, J. R., eds.) pp. 219–230, North Holland Publishing Company, Amsterdam.

    Google Scholar 

  18. Geelen, M. J. H., Beynen, A. C., Christiansen, R. Z., Le- preau-Jose, M.J. and Gibson, D. M., 1978. FEBS Lett. 95, 326330.

    Google Scholar 

  19. Beynen, A. C., Vaartjes, W. J. and Geelen, M. J. H. 1979. Diabetes 28, 828–835.

    Article  PubMed  CAS  Google Scholar 

  20. Dugan, R. E., Slakey, L. L., Briedis, A. V. and Porter, J. W. 1972. Arch. Biochem. Biophys. 152, 21–27.

    Google Scholar 

  21. Majerus, P. W. and Kilburn, E. 1969. J. Biol. Chem. 244, 6254–6262.

    Google Scholar 

  22. Nakanishi, S. and Numa, S. 1970. Eur. J. Biochem. 16, 161–173.

    Article  PubMed  CAS  Google Scholar 

  23. Craig, M. C., Nepokroeff, C. M., Lakshmanan, M. R. and Porter, J. W. 1972. Arch. Biochem. Biophys. 152, 619–630.

    Google Scholar 

  24. Lornitzo, F. A., Drong, R. F., Katiyar, S. S. and Porter, J. W. 1981. Methods Enzymol. 71, 292–306.

    Article  PubMed  CAS  Google Scholar 

  25. Lornitzo, F. A., Katiyar, S. S., Puri, R. N. and Porter, J. W. 1981. J. Biol. Chem. 256, 8498–8505.

    PubMed  CAS  Google Scholar 

  26. Lowenstein, J. M. 1967. Biochem. Soc. Symp. 27, 61–86.

    Google Scholar 

  27. Greenspan, M. D. and Lowenstein, J. M. 1968. J. Biol. Chem. 243, 6273–6280.

    PubMed  CAS  Google Scholar 

  28. Vagelos, P. R., Alberts, A. W. and Martin, D. B. 1963. J. Biol. Chem. 238, 533–540.

    PubMed  CAS  Google Scholar 

  29. Bortz, W. M. and Lynen, F. 1963. Biochem. Z 337, 505–509.

    PubMed  CAS  Google Scholar 

  30. Halestrap, A. P. and Denton, R. M. 1974. Biochem. J. 142, 365–377.

    PubMed  CAS  Google Scholar 

  31. Spencer, A. F. and Lowenstein, J. M. 1967. Biochem. J. 103, 342–348.

    PubMed  CAS  Google Scholar 

  32. Allred, J. B. and Roehrig, K. L. 1972. Biochem. Biophys. Res. Commun. 46, 1135–1139.

    Article  PubMed  CAS  Google Scholar 

  33. Harris, R. A. 1975. Arch. Biochem. Biophys. 169, 168–180.

    Article  PubMed  CAS  Google Scholar 

  34. Bricker, L. A. and Levey, G. S. 1972. J. Biol. Chem. 247, 4914–4915.

    Google Scholar 

  35. Harris, R. A. and Yount, R. A. 1975. Lipids 10, 673–680.

    Article  PubMed  CAS  Google Scholar 

  36. Lent, B. A., Lee, K.-H. and Kim, K.-H. 1978. J. Biol. Chem. 253, 8149–8156.

    Google Scholar 

  37. Carlson, C. A. and Kim, K.-H. 1974. Arch. Biochem. Biophys. 164, 478–489.

    Article  PubMed  CAS  Google Scholar 

  38. Lee, K.-H. and Kim, K.-H. 1977. J. Biol. Chem. 252, 1748–1751.

    PubMed  CAS  Google Scholar 

  39. Carlson, C. A. and Kim, K.-H., 1974. Arch. Biochem. Biophys. 164, 490–501.

    Article  PubMed  CAS  Google Scholar 

  40. Carlson, C. A. and Kim, K.-H. 1973. J. Biol. Chem. 248, 378–380.

    PubMed  CAS  Google Scholar 

  41. Shiao, M.-S., Drong, R. F. and Porter, J. W. 1981. Biochem. Biophys. Res. Commun. 98, 80–87.

    Article  PubMed  CAS  Google Scholar 

  42. Lent, B. and Kim, K.-H. 1982. J. Biol. Chem. 257, 1897–1901.

    Google Scholar 

  43. Geelen, M. J. H. and Gibson, D. M. 1975. FEBS Lett. 58, 334–339.

    Article  PubMed  CAS  Google Scholar 

  44. Witters, L. A., Moriarity, D. and Martin, D. B. 1979. J. Biol. Chem. 254, 6644–6649.

    PubMed  CAS  Google Scholar 

  45. Saccone, G. and Chang, C.-M. 1977. Fed. Proc. 36, 800 Abstr.

    Google Scholar 

  46. Witters, L. A., Kowaloff, E. M. and Avruch, J. 1979. J. Biol. Chem. 254, 245–248.

    Google Scholar 

  47. Witters, L. A. 1981. Biochem. Biophys. Res. Commun. 100, 872–878.

    Article  PubMed  CAS  Google Scholar 

  48. Geelen, M. J. H. and Beynen, A. C. 1981. In: Short-Term Regulation of Liver Metabolism (Hue, L. and Van de Werve, G., eds.), pp. 231–246, Elsevier/North Holland Biomedical Press, Amsterdam.

    Google Scholar 

  49. Song, C.-S. and Kim, K.-H. 1981. J. Biol. Chem. 256, 7786–7788.

    Google Scholar 

  50. Kowluru, R. A. and Porter, J. W. 1982. Diabetes 31 (Suppl. 2), 56.

    Google Scholar 

  51. Majerus, P. W., Jacobs, R., Smith, M. B. and Morris, N. P. 1968. J. Biol. Chem. 243, 3588–3595.

    Google Scholar 

  52. Davies, D. R., Van Schaftingen, E. and Hers, H.-G. 1982. Biochem. J. 202, 559–560.

    PubMed  CAS  Google Scholar 

  53. Inoue, H. and Lowenstein, J. M. 1975. Methods Enzymol. 35B, 3–11.

    Article  PubMed  CAS  Google Scholar 

  54. Myers, W.-F. and Huang, K. Y. 1969. 13, 431–434.

    Google Scholar 

  55. Allred, J. B. and Roehrig, K. L. 1973. J. Biol. Chem. 248, 4131–4133.

    Google Scholar 

  56. Assimacopoulos-Jeannet, F., Denton, R. M. and Jeanre- naud, B. 1981. Biochem. J. 198, 485–490.

    PubMed  CAS  Google Scholar 

  57. Buechler, K. F., Beynen, A. C. and Geelen, M. J. H. 1982. Fed. Proc. 41, 1192 (Abstr.).

    Google Scholar 

  58. Dorsey, J. K. and Porter, J. W. 1968. J. Biol. Chem. 243, 4667–4670.

    Google Scholar 

  59. Aultman, K. S. and Mapes, J. P. 1982. Fed. Proc. 41, 1191 (Abstr.).

    Google Scholar 

  60. Numa, S. 1969. Methods Enzymol. 14, 9–16.

    Article  CAS  Google Scholar 

  61. Collins, J. M., Craig, M. C., Nepokroeff, C. M., Kennan, A. L. and Porter, J. W., 1971. Arch. Biochem. Biophys. 143, 343–353.

    Google Scholar 

  62. Burton, D. N., Collins, J. M., Kennan, A. L. and Porter, J. W. 1969. J. Biol. Chem. 244. 4510–4516.

    Google Scholar 

  63. Craig, M. C., Dugan, R. E., Muesing, R. A., Slakey, L. L. and Porter, J. W. 1972. Arch. Biochem. Biophys. 151, 128–136.

    Google Scholar 

  64. Lakshmanan, M. R., Nepokroeff, C. M. and Porter, J. W. 1972. Proc. Natl. Acad. Sci. USA 69, 3516–3519.

    Google Scholar 

  65. Craig, M. C. and Porter, J. W. 1973. Arch. Biochem. Biophys. 159, 606–614.

    Google Scholar 

  66. Nakanishi, S., Tanabe, T., Horikawa, S. and Numa, S. 1976. Proc. Natl. Acad. Sci. USA 73, 2304–2307.

    Article  PubMed  CAS  Google Scholar 

  67. Nepokroeff, C. M., Lau, H.-P. and Porter, J. W. 1979. Int. J. Biochem. 10, 791–796.

    Article  PubMed  CAS  Google Scholar 

  68. Horikawa, S., Nakanishi, S. and Numa, S. 1977. FEBS Lett. 74, 55–58.

    Article  PubMed  CAS  Google Scholar 

  69. Alberts, A. W., Strauss, A. W., Hennessy, S. and Vagelos, P. R. 1975. Proc. Natl. Acad. Sci. USA 72, 3956–3960.

    Google Scholar 

  70. Strauss, A. W., Alberts, A. W., Hennessy, S. and Vagelos, P. R. 1975. Proc. Natl. Acad. Sci. USA 72, 4366–4370.

    Article  PubMed  CAS  Google Scholar 

  71. Nepokroeff, C. M. and Porter, J. W. 1978. J. Biol. Chem. 253, 2279–2283.

    Google Scholar 

  72. Flick, P. K., Chen, J., Alberts, A. W. and Vagelos, P. R. 1978. Proc. Natl. Acad. Sci. USA 75, 730–734.

    Article  PubMed  CAS  Google Scholar 

  73. Pry, T. A. and Porter, J. W. 1981. Biochem. Biophys. Res. Commun. 100, 1002–1009.

    Google Scholar 

  74. Katz, N. R. and Ick, M. 1981. Biochem. Biophys. Res. Commun. 100, 703–709.

    Article  PubMed  CAS  Google Scholar 

  75. Nepokroeff, C. M., Lakshmanan, M. R., Ness, G. C., Muesing, R. A., Kleinsek, D. A. and Porter, J. W. 1974. Arch. Biochem. Biophys. 162, 340–344.

    Article  PubMed  CAS  Google Scholar 

  76. Roncari, D. A. K. and Murthy, V. K. 1975. J. Biol. Chem. 250, 4134–4138.

    PubMed  CAS  Google Scholar 

  77. Kumar, S., Das, D. K., Dorfman, A. E. and Asato, N., 1977. Arch. Biochem. Biophys. 178, 507–516.

    Article  PubMed  CAS  Google Scholar 

  78. Das, D. K. 1980. Arch. Biochem. Biophys. 203, 25–36.

    Article  PubMed  CAS  Google Scholar 

  79. Volpe, J. J. and Marasa, J. C. 1975. Biochem. Biophys. Acta 380, 454–472.

    PubMed  CAS  Google Scholar 

  80. Kitajima, K., Tashior, S. and Numa, S., 1975. Eur. J. Bio-chem. 54, 373–383.

    CAS  Google Scholar 

  81. Allman, D. W., Hubbard, D. D. and Gibson, D. M. 1965. J. Lipid Res. 6, 63–74.

    Google Scholar 

  82. Gibson, D. M., Hicks, S. E. and Allman, D. W. 1966. Adv. Enzyme Regul. 4, 239–246.

    Article  CAS  Google Scholar 

  83. Adachi, K., Pry, T. A., Nepokroeff, C. M. and Porter, J. W. 1982. Biochim. Biophys. Acta 697: 295–304.

    PubMed  CAS  Google Scholar 

  84. Yu, H. L. and Burton, D. N. 1974. Arch. Biochem. Biophys. 161, 297–305.

    Article  CAS  Google Scholar 

  85. Burton, D. N., Sobhy, C., Jenik, R. A. and Porter, J. W. 1979. Methods Enzymol. 62, 249–262.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Physiological Chemistry, University of Wisconsin, Madison, WI, 53705, USA

    John W. Porter & Theresa L. Swenson

  2. Lipid Metabolism Laboratory, William S. Middleton Memorial Veterans Hospital, Madison, WI, 53705, USA

    John W. Porter & Theresa L. Swenson

Authors
  1. John W. Porter
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Theresa L. Swenson
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Division of Protein Chemistry, Tufts University School of Medicine, Boston, Massachusetts, USA

    V. A. Najjar

Rights and permissions

Reprints and permissions

Copyright information

© 1983 Martinus Nijhoff Publishers, Boston

About this chapter

Cite this chapter

Porter, J.W., Swenson, T.L. (1983). Induction of fatty acid synthetase and acetyl-CoA carboxylase by isolated rat liver cells. In: Najjar, V.A. (eds) Enzyme Induction and Modulation. Developments in molecular and cellular biochemistry, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3879-6_20

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-3879-6_20

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-3881-9

  • Online ISBN: 978-1-4613-3879-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation