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3T3-L1 preadipocyte differentiation and poly(ADP-ribose) synthetase

  • Phillip H. Pekala
  • Joel Moss
Part of the Developments in molecular and cellular biochemistry book series (DMCB, volume 3)

Summary

Differentiation of 3T3-L1 preadipocytes, induced by methyl-isobutylxanthine (MIX), dexamethasone (DEX), and insulin, results in cells with the morphological and biochemical characteristics of adipocytes. Following incubation of 3T3-L1 cells with MIX, DEX, and insulin, poly(ADP-ribose) synthetase activity decreased abruptly, remained low for several hours and then increased; this rise was delayed by readdition of MIX, DEX, and insulin. The transient reduction in poly(ADP-ribose) synthetase activity in 3T3-L1 cells occurred prior to the appearance of the adipocyte phenotype induced by the above agents. It was not observed when preparations were assayed in the presence of DNase I, indicating that poly(ADP-ribose) synthetase activity was masked following treatment with MIX, DEX, and insulin.

The change in synthetase activity represents the earliest alteration of a specific enzyme yet detected during the differentiation of 3T3-L1 cells. It appears to be differentiation specific since nondifferentiating 3T3-C2 control cells did not exhibit changes in poly(ADP-ribose) synthetase activity when treated with MIX, DEX, and insulin. The transient reduction in activity may be an early event in differentiation which reflects changes in chromatin structure.

Keywords

Chromatin Structure Particulate Fraction Undifferentiated Cell Synthetase Activity Transient Reduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Martinus Nijhoff Publishers, Boston 1983

Authors and Affiliations

  • Phillip H. Pekala
    • 1
  • Joel Moss
    • 2
  1. 1.Dept. of BiochemistryEast Carolina University School of MedicineGreenvilleUSA
  2. 2.Laboratory of Cellular Metabolism National Heart, Lung, and Blood InstituteBethesdaUSA

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