Cell types of endocrine pancreas by immunoelectron microscopy

  • Georges Pelletier
Part of the Electron Microscopy in Biology and Medicine book series (EMBM, volume 1)


The first light microscopic studies using differential staining have established that the endocrine pancreas (also named islets of Langerhans) of a large number of mammals contained at least three different cell types (1,2). In the early days of electron microscopy, two cell types, the alpha and the beta cell, were identified by using ultrathin sections adjacent to thick sections specifically stained for light microscopic investigation (3–5). Later on, the delta cell was identified on the basis of its location in the islet and on the ultrastructural characteristics of its secretory granules which appeared different from those of alpha and beta cells (6–10). In the seventies, the rapid development of immunocytochemical techniques, especially those applied to the ultrastructural localization of antigens (11), as well as the discovery of new pancreatic hormones (12, 13), has largely contributed to an easier identification of the cell types and organelles containing the different hormones of the endocrine pancreas. In this chapter, we have summarized what is presently known about the identification and classification of the different cell types of the mammalian endocrine pancreas.


Beta Cell Pancreatic Islet Secretory Granule Dense Core Pancreatic Polypeptide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Hartroft WS, Wrenshall G A: Correlation of beta cell granulation with extractable insulin of the pancreas. Diabetes 4: 1–7, 1955.PubMedGoogle Scholar
  2. 2.
    Hellerstrom C, Hellman B: Some aspects of silver impregnation of the islets of Langerhans in the rat. Acta Endocrinol 35: 518–532, 1960.Google Scholar
  3. 3.
    Bencosme SA, Peace DC: Electron microscopy of the pancreatic islets. Endocrinology 63: 1–13, 1958.PubMedCrossRefGoogle Scholar
  4. 4.
    Lacy PE: Electron microscopy of the normal islets of Langerhans: studies in the dog, rabbit, guinea pig and rat. Diabetes 6: 498–507, 1957.PubMedGoogle Scholar
  5. 5.
    Lacy PE: Electron microscopy of the beta cell of the pancreas. Am J Med 31: 851–859, 1961.PubMedCrossRefGoogle Scholar
  6. 6.
    Sato TL, Herman, Fitzgerald PJ: The comparative ultrastructure of the pancreatic islet of Langerhans. Gen Comp Endocrinol 7: 132–157, 1966.CrossRefGoogle Scholar
  7. 7.
    Bjorkman N, Hellerstrom C, Hellman B, Petersson B: The cell types in the endocrine pancreas of the human fetus. Z Zellforsch 72: 425–445, 1966.PubMedCrossRefGoogle Scholar
  8. 8.
    Caramia F: Electron microscopic description of a third cell type in the islets of the rat pancreas. Am J Anat 112: 53–64, 1963.PubMedCrossRefGoogle Scholar
  9. 9.
    Caramia F, Munger BL, Lacy PE: The ultrastructural basis for the identification of cell types in the pancreatic islets. I. Guinea pig. Z Zellforsch 67: 533–546, 1965.PubMedCrossRefGoogle Scholar
  10. 10.
    Greider MH, Bencosme SA, Lechago J: The human pancreatic islet cells and their tumors. I. The normal pancreatic islets. Lab Invest 22: 344–354, 1970.PubMedGoogle Scholar
  11. 11.
    Sternberger LA: Immunocytochemistry. Englewood Cliffs, NJ, Prentice-Hall, 1974.Google Scholar
  12. 12.
    Brazeau P, Vale W, Burgus R, Ling N, Butcher M, Rivier J, Guillemin R: Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. i 179: 77–79, 1973.Google Scholar
  13. 13.
    Lin TM, Chance RE: The biology of secretory tumors of the pancreatic islets. In: Endocrinology of the Gut Cheng WY, Brooks FP (eds), Charles B Slack Inc 1974, pp 143–145.Google Scholar
  14. 14.
    Pelletier G: Identification of four cell types in the human endocrine pancreas by immunoelectron microscopy. Diabetes 26: 749–756, 1977.PubMedCrossRefGoogle Scholar
  15. 15.
    Pelletier G: Immunohistochemical localization of somatostatin. In: Progress in Histochemistry and Cytochemistry, vol. 12, Gustav Fischer (ed) Verlag, Stuttgart, New York, 1979, pp 140.Google Scholar
  16. 16.
    Ranazzola M. Siperstein A, Moody AJ, Sundy F, Jacobsen H, Orci L: Glicentin immunoreactive cells: their relationship to glucagon-producing cells. Endocrinology 105: 499 - 508, 1979.CrossRefGoogle Scholar
  17. 17.
    Lomsky R, Longr F, Vortel V: Immunohistochemical demonstration of gastrin in mammalian islets of Langerhans. Nature 223: 618–619, 1969.PubMedCrossRefGoogle Scholar
  18. 18.
    Greider MH, McGuigan JE: A comparison of ulcerogenic tumors of the pancreas with the gastrin cell of normal human pancreas and hog antrum. Am J Pathol 59: 76–77, 1970.Google Scholar
  19. 19.
    Creutzfeldt W, Creutzfeldt C, Arnold R: Gastrin-producing cells. In: Endocrinology of the Gut. Cheng WY, Brooks FP, Thorofare, NJ (eds), Charles B Slack Inc, 1975, pp 35–62.Google Scholar
  20. 20.
    Orci L, Unger RH: Functional subdivision of islets of Langerhans and possible role of D-cells. Lancet 2: 1243–1244, 1975.PubMedCrossRefGoogle Scholar
  21. 21.
    Pelletier G, Leclerc R, Arimura A, Schally AV: Immunohistochemical localization of somatostatin in the rat pancreas. J Histochem Cytochem 23: 699–701, 1975.PubMedCrossRefGoogle Scholar
  22. 22.
    Dubois MP: Immunoreactive somatostatin is present in discrete cells of the endocrine pancreas. Proc Natl Acad Sci (USA) 72: 1340–1343, 1975.CrossRefGoogle Scholar
  23. 23.
    Goldsmith PC, Rose JC, Arimura A, Ganong WF: Ultrastructural localization of somatostatin in pancreatic islets of the rat. Endocrinology 97: 1061–64, 1975.PubMedCrossRefGoogle Scholar
  24. 24.
    Lacy PE, Greider MH: Ultrastructural organization of mammalian pancreatic islets. In: Handbook of Physiology, vol. 1, Endocrine Pancreas, Greep RO, Astwood ER (eds). Am Phys Soc, Washington DC, 1972, pp 77–89.Google Scholar
  25. 25.
    Pelletier G, Leclerc R: Immunohistochemical localization of human pancreatic polypeptide (HPP) in the human endocrine pancreas. Gastroenterology 72: 569–571, 1977.PubMedGoogle Scholar
  26. 26.
    Greider MA, Gersell DJ, Gingerish RL: Ultrastructural localization of pancreatic polypeptide in the F cell of the dog pancreas. J Histochem Cytochem 26, 1103–1108, 1978.PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishers, Boston, The Hague, Dordrecht, Lancaster 1984

Authors and Affiliations

  • Georges Pelletier
    • 1
  1. 1.MRC Group in Molecular EndocrinologyLe Centre Hospitalier de l’Université LavalCanada

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