Abstract
4-Hydroxy-4-androstene-3,17-dione (40HA) is an effective inhibitor of aromatase in vitro whilst in vivo it acts as an anti-estrogen (Brodie et al, Endocrinol. 100: 1684, 1977). The metabolism of (14C) 4-OHA was studied using rat hepatocytes (prepared by perfusion technique). After incubation at 37°C for 1hr, 70% of the total radioactivity added was in the aqueous residue remaining after extraction with ethyl acetate. Steroid constituents of the aqueous phase were absorbed on to amberlite X AD-2 resin, eluted with MeOH and separated by TLC. The only area of radioactivity on the TLC plate was associated with a band which stained strongly positive with a naphthoresorcinol spray reagent specific for glucuronide.
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© 1984 Martinus Nijhoff Publishing, Boston
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Parr, I.B., Rowlands, M.G. (1984). Metabolism of 4-Hydroxyandrostene-3, 17-Dione by Rat Liver. In: Harrap, K.R., Davis, W., Calvert, A.H. (eds) Cancer Chemotherapy and Selective Drug Development. Developments in Oncology, vol 23. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3837-6_122
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DOI: https://doi.org/10.1007/978-1-4613-3837-6_122
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