Use of Repair-Deficient Mammalian Cells for the Identification of Carcinogens
The most prominent cellular effects of DNA-damaging agents are cytotoxicity (cell killing), mutagenesis, and the induction of cytogenetic changes including chromosomal aberrations and sister chromatid exchanges. Although bioassays for environmental carcinogens in mammalian cells have employed several of these end points, mutagenesis has generally been considered the one most intimately involved in the process of carcinogenesis. In general terms, the use of hypermutable cells should improve the sensitivity of a bioassay for a carcinogen which is based on its mutagenic potential, particularly if such lines were relatively insensitive to the cytotoxic effects of the carcinogen.
KeywordsExcision Repair Xeroderma Pigmentosum Ethyl Methane Sulfonate Sensitive Bioassay Bulky Adduct
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