Abstract
The influence of chromatin structure on the binding of benzo- (a)pyrene metabolites to the genome is studied. Carcinogen modified regions, in cellular chromatin, in restriction fragments of SV40, and in polytene chromosomes are detected using antibodies elicited by [r-7, t-8-dihydroxy-t-9, 10-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene] BPDE-modified DNA. A method has been developed which allows detection of adducts in defined restriction fragments derived from DNA molecules modified to less than one carcinogen adduct per DNA molecule, Polytene chromosomes allow direct visualization of carcinogen binding regions. Autoradiography of 3H-BPDE-treated chromosomes followed by immunofluorescence in the presence or absence of RNase allows distinction between the binding to DNA, RNA, and proteins. The results indicate that the nucleosomal structure of chromatin has a very slight effect on the binding of the carcinogen. However, certain loci in the polytene chromosomes display a significant preference for anti-BPDE binding.
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© 1983 Plenum Press, New York
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Bustin, M., Kurth, P.D., Slor, H., Seidman, M. (1983). Immunological Studies on the Influence of Chromatin Structure on the Binding of a Chemical Carcinogen to the Genome. In: Milman, H.A., Sell, S. (eds) Application of Biological Markers to Carcinogen Testing. Environmental Science Research, vol 29. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3790-4_28
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DOI: https://doi.org/10.1007/978-1-4613-3790-4_28
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