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Human Tumors: Enzyme Activities And Isozyme Profiles

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Application of Biological Markers to Carcinogen Testing

Part of the book series: Environmental Science Research ((ESRH,volume 29))

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Abstract

My own interest in tumor metabolism was sparked by the unique availability in South Africa, where I was located at the time, of human hepatoma specimens. Human primary hepatoma has a fairly low incidence in Western countries, but an incidence of 1:1,100 in Mozambique. We were able to obtain hepatoma material from migrant workers, mostly from Mozambique, who showed evidence of the disease while working in South Africa. Since I have been in the United States, I have extended these studies to human breast and lung tumors, and found, as I will indicate to you, that they do not show the same enzyme and isozyme profiles. This is an important point to note, since too often generalizations are made about “cancer”, as if all cancers were the same, irrespective of cause or tissue of origin.

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References

  1. Weber, G., and M.A. Lea (1966) The molecular correlation concept of neoplasia. Adv. Enz. Reg. 4:115–145.

    Article  Google Scholar 

  2. Weber, G., S.F. Queener, and J.A. Ferdinandus (1971) Control of gene expression in carbohydrate, pyrimidine and DNA metabolism. Adv. Enz. Reg. 9:63–95.

    Article  Google Scholar 

  3. Sneider, T.W., V.R. Potter, and H.P. Morris (1969) Enzymes of thymidine triphosphate synthesis in selected Morris hepatomas. Cancer Res. 29:40–54.

    Google Scholar 

  4. Cummins, R.R., and D. Balinsky (1980) Activities of some enzymes of pyrimidine and DNA synthesis in a rat transplantable hepatoma and human primary hepatomas, in cell lines derived from these tissues, and in human fetal liver. Cancer Res. 40:1235–1239.

    Google Scholar 

  5. Gordon, H.L., T.J. Bardos, Z.F. Chmielewicz, and J.L. Ambrus (1968) Comparative study of the thymidine kinase and thymidylate kinase activities and of the feedback inhibition of thymidine kinase in normal and neoplastic human tissue. Cancer Res. 28:2068–2077.

    Google Scholar 

  6. Laszlo, J., P. Ove, W.W. Shingleton, and H.P. Morris (1969) DNA replication and degradation in mammalian tissues. V. A series of slow-growing rat renal tumors and a variety of human cancers. J. Natl. Cancer Inst. 43:1331–1336.

    Google Scholar 

  7. Maehara, Y., H. Nakamura, Y. Nakane, K. Kawai, S. Okamoto, S. Nagayama, T. Shirasaka, and S. Fujii (1982) Activities of various enzymes of pyrimidine nucleotide and DNA synthesis in normal and neoplastic human tissues. Gann 73:289–298.

    Google Scholar 

  8. Taylor, A.T., M.A. Stafford, and O.W. Jones (1972) Properties of thymidine kinase partially purified from human fetal and adult tissue. J. Biol. Chem. 247:1930–1935.

    Google Scholar 

  9. Cummins, R.R., and D. Balinsky (1979) Thymidine kinases from normal human liver and hepatoma. Isozyme Bull. 12:36.

    Google Scholar 

  10. Baril, E., and J. Laszlo (1970) Sub-cellular localization and characterization of DNA polymerases from rat liver and hepatomas. Adv. Enz. Reg. 9:183–204.

    Article  Google Scholar 

  11. Warburg, O., K. Posener, and E. Negelein (1924) Uber den Stoffwechsel der Carcinomzelle. Biochem. Z. 152:309–344.

    Google Scholar 

  12. Morris, H.P., and B.P. Wagner (1968) Induction and transplantation of rat hepatomas with different growth rate (including “minimal deviation” hepatomas). Methods Cancer Res. 4:125–152.

    Google Scholar 

  13. Elwood, J.C., Y.C. Lin, V.J. Cristofalo, S. Weinhouse, and H.P. Morris (1963) Glucose utilization in homogenates of the Morris hepatoma 5123 and related tumors. Cancer Res. 23:906–913.

    Google Scholar 

  14. Balinsky, D., E. Cayanis, E. Geddes, and I. Bersohn (1973) Activities and isoenzyme patterns of some enzymes of glucose metabolism in human primary malignant hepatoma. Cancer Res. 33:249–255.

    Google Scholar 

  15. Rosado, A., H.P. Morris, and S. Weinhouse (1969) Lactate dehydrogenase subunits in normal and neoplastic tissues of the rat. Cancer Res. 29:1673–1680.

    Google Scholar 

  16. Balinsky, D., O. Greengard, J.F. Head, E. Cayanis, and S.L. Goldberg (1983) Enzyme activities and isozyme patterns in human lung tumors (submitted for publication).

    Google Scholar 

  17. Balinsky, D., C.P. Platz, and J.W. Lewis (1983) Enzyme activities in normal, benign and malignant human breast tissues (submitted for publication).

    Google Scholar 

  18. Hilf, R., J.L. Wittliff, W.D. Rector, E.D. Savlov, T.C. Hall, and R.A. Orlando (1973) Studies on certain cytoplasmic enzymes and specific estrogen receptors in human breast cancer and in nonmalignant diseases of the breast. Cancer Res. 33:2054–2062.

    Google Scholar 

  19. Hammond, K.D., and D. Balinsky (1978) Activities of key gluconeogenic enzymes and glycogen synthetase in rat and human livers, hepatomas and hepatoma cell cultures. Cancer Res. 38:1317–1322.

    Google Scholar 

  20. Goldman, R.D., N.O. Kaplan, and T.C. Hall (1964) Lactic dehydrogenase in human neoplastic tissues. Cancer Res. 24:389–399.

    Google Scholar 

  21. Hilf, R., W.D. Rector, and R.A. Orlando (1976) Multiple molecular forms of lactate dehydrogenase and glucose 6-phosphate dehydrogenase in normal and abnormal human breast tissues. Cancer 37:1825–1830.

    Article  Google Scholar 

  22. Kaplan, N.O., and T.L. Goodfriend (1964) Role of the two types of lactic dehydrogenase. Adv. Enz. Reg. 2:203–212.

    Article  Google Scholar 

  23. Schapira, F., C. Micheau, and C. Junien (1972) Rev. Eur. Etud. Clin. Biol. 17:896.

    Google Scholar 

  24. Siciliano, M.J., M.E. Bordelon-Riser, R.S. Freedman, and P.O. Kohler (1980) A human trophoblastic isozyme (lactate dehydrogenases) associated with choriocarcinoma. Cancer Res. 40:283–287.

    Google Scholar 

  25. Anderson, G.R., and W.P. Kovacik, Jr. (1981) LDHK, an unusual oxygen-sensitive lactate dehydrogenase expressed in human cancer. Proc. Natl. Acad. Sci., USA 78:3209–3213.

    Article  ADS  Google Scholar 

  26. Hershey, F.B., G. Johnston, S.M. Murphy, and M. Schmitt (1966) Pyridine nucleotide-linked dehydrogenases and isozymes of normal rat breast and growing and regressing breast cancers. Cancer Res. 26:265–268.

    Google Scholar 

  27. Ibsen, K.H. (1977) Interrelationships and functions of pyruvate kinase isozymes and their variant forms: A review. Cancer Res. 37:341–353.

    Google Scholar 

  28. Blair, J.B. (1981) Regulatory properties of hepatic pyruvate kinase. In The Regulation of Carbohydrate Formation and Utilization in Mammals, C.M. Veneziale, ed. University Park Press, Baltimore pp. 121–151.

    Google Scholar 

  29. Imamura, K., and T. Tanaka (1972) Multimolecular forms of pyruvate kinase from rat and other mammalian tissues: I. Electrophoretic studies. J. Biochem. (Tokyo) 71:1043–1051.

    Google Scholar 

  30. Noguchi, T., and T. Tanaka (1982) The M. and M2 subunits of rat pyruvate kinase are encoded by different messenger RNAs. J. Biol. Chem. 257:1110–1113.

    Google Scholar 

  31. Marie, J., M.P. Simon, J.C. Dreyfus, and A. Kahn (1981) On gene, but two messenger RNAs encode liver L and red cell L pyruvate kinase subunits. Nature 292:70–72.

    Article  ADS  Google Scholar 

  32. Marie, J., H. Garreau, and A. Kahn (1977) Evidence for a postsynthetic proteolytic transformation of human erythrocyte pyruvate kinase into L-type enzyme. FEBS Lett., 78:91–94.

    Article  Google Scholar 

  33. Balinsky, D., E. Cayanis, and I. Bersohn (1973) Comparative kinetic properties of human pyruvate kinases isolated from adult and fetal livers and from hepatoma. Biochemistry 12:863–870.

    Article  Google Scholar 

  34. Balinsky, D., E. Cayanis, C.F. Albrecht, and I. Bersohn (1972) Enzymes of carbohydrate metabolism in rat hepatoma induced by 3?-methyl-4-dimethylaminoazobenzene (3f-MeDAB). South African J. Med. Sci. 37:95–99.

    Google Scholar 

  35. Walker, P.R. and V.R. Potter (1972) Isozyme studies on adult, regenerating, precancerous and developing liver in relation to findings in hepatomas. Adv. Enz. Reg. 10:339–364.

    Article  Google Scholar 

  36. Farina, F.A., J.B. Shatton, H.P. Morris, and S. Weinhouse (1974) Isozymes of pyruvate kinase in liver and hepatomas of the rat. Cancer Res. 34:1439–1446.

    Google Scholar 

  37. Van Veelen, C.V.M., H. Verbiest, A.M.C. Vlug, G. Rijksen, and G.E.J. Staal (1978) Isozymes of pyruvate kinase from human brain, meningiomas, and malignant gliomas. Cancer Res. 38:4861–4867.

    Google Scholar 

  38. van Veelen, C.W.M., H. Verbiest, K. Ziilch, B.A. van Ketel, M.J.M. van der Vist, A.M.C. Vlug, G. Rijksen, and G.E.J. Staal (1979) L-alpha-alanine inhibition of pyruvate kinase from tumors of the human central nervous system. Cancer Res. 39:4263–4269.

    Google Scholar 

  39. Beemer, F.A. A.M.C. Vlug, G. Rijksen, and G.E.J. Staal (1982) Hexokinase, pyruvate kinase and aldolase from fetal and adult retina, retino-blastoma cell line, and retinoblastoma. Proc. 4th Intl. Conf. on Isozymes, Austin, Texas.

    Google Scholar 

  40. Lopez-Alarcon, L., P. Ruiz, and M. Gosalvez (1981) Determination of the degree of differentiation of mammary tumors by pyruvate kinase analysis. Cancer Res. 41:2019–2020.

    Google Scholar 

  41. Ibsen, K.H., R.A. Orlando, K.N. Garratt, A.M. Hernandez, S. Giorlando, and G. Nungaray (1982) Expression of multimolecular forms of pyruvate kinase in normal, benign and malignant human breast tissue. Cancer Res. 42:888–892.

    Google Scholar 

  42. Kamel, R., and F. Schwarzfischer (1975) Hexokinase isozymes in human neoplastic and fetal tissues: The existence of hexokinase II in malignant tumors and in placenta. Humangenetik 30:181–185.

    Article  Google Scholar 

  43. Sato, S., T. Matsushima, and T. Sugimura (1969) Hexokinase isozyme patterns of experimental hepatomas of rats. Cancer Res. 29:1437–1446.

    Google Scholar 

  44. Weinhouse, S., J.B. Shatton, W.E. Criss, and H.P. Morris (1972) Isozymes in relation to differentiation in transplantable rat hepatomas. Gann Monograph 13:1–17.

    Google Scholar 

  45. Hatzfeld, A., A. Weber, and F. Schapira (1975) Biochemical and immunological studies of some carcinofetal enzymes. Ann. N.Y. Acad. Sci. 259:287–297.

    Article  ADS  Google Scholar 

  46. Hatzfeld, A., G. Feldmann, J. Guesnon, C. Fraysinnet, and F. Schapira (1978) Location of adult and fetal aldolases A, B, and C by immunoperoxidase technique in LF fast-growing rat hepatomas. Cancer Res. 38:16–22.

    Google Scholar 

  47. Fishman, W.H., N.R. Inglis, L.L. Stolbach, and M.J. Kraut (1968) A serum alkaline phosphatase isoenzyme of human neoplastic cell origin. Cancer Res. 28:150–154.

    Google Scholar 

  48. Fishman, W.H. (1974) Perspectives on alkaline phosphatase isoenzymes. Amer. J. Med. 56:617–650.

    Article  Google Scholar 

  49. Nakayama, T., M. Yoshida, and M. Kitamura (1970) L-leucine sensitive, heat-stable alkaline-phosphatase isoenzyme detected in a patient with pleuritis carcinomatosa. Clin. Chim. Acta 30:546–548.

    Article  Google Scholar 

  50. Higashino, K., M. Hashinotsume, K.-Y Yang, Y. Takahashi, and Y. Yamamura (1972) Studies on a variant alkaline phosphatase in sera of patients with hepatocellular carcinoma. Clin. Chim. Acta 40:67–81.

    Article  Google Scholar 

  51. Fishman, L., H. Miyahama, S.G. Driscoll, and W.H. Fishman (1976) Developmental phase-specific alkaline phosphatase isoenzymes of human placenta and their occurrence in human cancer. Cancer Res. 36:2268–2273.

    Google Scholar 

  52. Fishman, W.H., T. Nishiyama, A. Rule, S. Green, N.R. Inglis, and L. Fishman (1976) Onco-developmental alkaline phosphatase isozymes. In Onco-Developmental Gene Expression, W.H. Fishman and S. Sell, eds. Academic Press, New York, pp. 165–176.

    Google Scholar 

  53. Balinsky, D., P.S. Jenkins, C.E. Platz, J.W. Lewis, and M.C. Kew (1981) Esterases in human cancer. Proc. Amer. Assoc. Cancer Res. 22:35.

    Google Scholar 

  54. Coates, P.M., M.A. Mestriner, and D.A. Hopkinson (1975) A preliminary genetic interpretation of the esterase isozymes of human tissues. Ann. Hum. Genet. 39:1–20.

    Article  Google Scholar 

  55. Guillouzo, A., A. Weber, E. LeProvost, M. Rissel, and F. Schapira (1981) Cell-types involved in the expression of foetal aldolases during rat azo-dye hepatocarcinogenesis. J. Cell. Sci. 49:249–260.

    Google Scholar 

  56. LeProvost, E., A. Weber, F. Schapira, M. Boisnard-Rissel, and A. Guillouzo (1980) Immunolocalization of fetal aldolase isoenzymes in rat regenerating liver after carbon tetrachloride intoxication. Oncodevelop. Biol. Med. 1:263–272.

    Google Scholar 

  57. Herzfeld, A., and 0. Greengard (1972) Effect of neoplasms on the content and activity of alkaline phosphatase and gamma-glutamyl transpeptidase in uninvolved host tissues. Cancer Res. 32:1826–1832.

    Google Scholar 

  58. Suda, M., T. Tanaka, S. Yanagi, S. Hayashi, K. Imamura, and K. Taniuchi (1972) Dedifferentiation of enzymes in the liver of tumor-bearing animals. Gann Monograph 13:79–93.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Iowa State University, 50011, Ames, Iowa, USA

    Doris Balinsky

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  1. Doris Balinsky
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Editors and Affiliations

  1. United States Environmental Protection Agency, Washington, D.C., USA

    Harry A. Milman

  2. University of Texas Health Science Center, Houston, Texas, USA

    Stewart Sell

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© 1983 Plenum Press, New York

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Balinsky, D. (1983). Human Tumors: Enzyme Activities And Isozyme Profiles. In: Milman, H.A., Sell, S. (eds) Application of Biological Markers to Carcinogen Testing. Environmental Science Research, vol 29. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3790-4_19

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  • DOI: https://doi.org/10.1007/978-1-4613-3790-4_19

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-3792-8

  • Online ISBN: 978-1-4613-3790-4

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