Abstract
Most hepatocarcinogenesis models assume that chemical carcinogens (or their reactive metabolites) bind to hepatocyte DNA and, from mutations caused by this event, “initiate” malignant transformation (1). Recent studies of oncogene transfection (2), structure (3), and expression in normal regenerating rat liver (4) tend to strengthen — but not prove (5) — these concepts. For example, liver stem cells might instead be tumor progenitors and macromolecules besides DNA might instead be initiating “targets” (6). Nonetheless, if the “hepatocyte DNA target” point of view prevails, long-term primary cultures of such adult cells will be useful to analyze the molecular and cellular basis of chemical carcinogenesis in normal epithelial systems. We have been working since 1970 to develop a system of this kind. We highlight, here, some of our results along these lines (see also refs. 7–11). Detailed accounts, mainly with 2-acetyl-aminofluorene (AAF), will appear elsewhere (12–15).
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© 1983 Plenum Press, New York
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Leffert, H.L., Koch, K.S., Sell, S., Skelly, H., Shier, W.T. (1983). Biochemistry and Biology of 2-Acetyl-Aminofluorene in Primary Cultures of Adult Rat Hepatocytes. In: Milman, H.A., Sell, S. (eds) Application of Biological Markers to Carcinogen Testing. Environmental Science Research, vol 29. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3790-4_11
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DOI: https://doi.org/10.1007/978-1-4613-3790-4_11
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