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Protein Conformation as an Immunological Signal pp 3–13Cite as

Antibody-Hapten Binding Kinetics, Conformational Transitions and Domains Interactions

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Abstract

Upon binding antigen, gross changes primarily in the spatial relation of the Fab domains relative to that of the Fc are expected to occur in the antibody structure 1,2. The question whether conformational changes are caused in the combining site has been of great concern in the context of understanding the mode of effector function activation mechanism3–5. Therefore, the effect induced in antibodies by binding their haptens or even larger parts of their respective antigens, has been examined by a wide range physical and chemical methods3–5. The direct structural studies by crystallography failed to detect differences between the free combining sites and their complexes with haptens in the two cases examined to date6–7. In contrast several spectroscopic and other physical methods provided clear evidence for structural changes induced by hapten binding8–10. In the following the results of the time resolved analysis of antibody-hapten reaction will be described in detail. The kinetic studies of these reactions provided a very detailed insight into the elementary steps of hapten recognition by the antibody combining site. Furthermore, conclusive evidence for a conformational change induced upon hapten binding emerged from these investigations11–14.

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Author information

Authors and Affiliations

  1. The Weizmann Institute of Science, Rehovot, Israel

    Israel Pecht

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  1. Israel Pecht
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Editors and Affiliations

  1. University of Genoa, Genoa, Italy

    Franco Celada

  2. University of California, Los Angeles, California, USA

    Verne N. Schumaker  & Eli E. Sercarz  & 

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© 1983 Plenum Press, New York

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Pecht, I. (1983). Antibody-Hapten Binding Kinetics, Conformational Transitions and Domains Interactions. In: Celada, F., Schumaker, V.N., Sercarz, E.E. (eds) Protein Conformation as an Immunological Signal. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3778-2_2

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  • DOI: https://doi.org/10.1007/978-1-4613-3778-2_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-3780-5

  • Online ISBN: 978-1-4613-3778-2

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