Abstract
In the preceding chapters dealing with modifications of nucleic acids it is assumed that, in general, these chemical reactions are randomly located along the genome. Exceptions occur when protein-nucleic interactions or nucleic acid secondary structure playa role. When studying mutation in vivo, changes in phenotype or in the sequence of newly synthesized DNAs, RNAs, or protein have been used to detect the mutants. These methods can answer only the question of whether a particular reagent, usually modifying multiple nucleic acid sites, is a mutagen; if a cellular function is changed, sequence data may pinpoint the specific nucleic acid regions responsible.
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© 1983 Plenum Press, New York
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Singer, B., Grunberger, D. (1983). Site-Directed Mutagenesis. In: Molecular Biology of Mutagens and Carcinogens. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3772-0_9
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DOI: https://doi.org/10.1007/978-1-4613-3772-0_9
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