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Human Mutagenicity Monitoring: Studies with 6-Thioguanine Resistant Lymphocytes

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Utilization of Mammalian Specific Locus Studies in Hazard Evaluation and Estimation of Genetic Risk

Part of the book series: Environmental Science Research ((ESRH,volume 28))

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Abstract

The field of human mutagenicity testing is maturing, with a resultant refinement in concepts and objectives. The screening of new chemical agents in order to identify those which may cause genetic damage is now only one form of mutagenicity testing. Screening tests attempt to identify the mutagens among new chemicals in hopes of achieving the ideal — i.e., the identification and elimination of dangerous chemicals before humans become exposed to them. By contrast, there are now also monitoring tests. As the name implies, these tests permit the monitoring of human populations for evidence of genetic damage resulting from mutagens present in the environment.

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References

  1. B. A. Bridges, The three-tier approach to mutagenicity screening and the concept of radiation-equivalent dose, Mutat. Res., 26:335–340 (1976).

    Google Scholar 

  2. EPA Document: EPA 625/9-79-003. Environmental Assessment: short term tests for carcinogens, mutagens and other genotoxic agents, July 1979.

    Google Scholar 

  3. C. F. Arlett and A. R. Lenman, Human disorders showing increased sensitivity to the induction of genetic damage, Ann. Rev. Genet., 12:95–115 (1978).

    Article  Google Scholar 

  4. M. Swift and C. Chase, Cancer in families with xeroderma pigmentosa, J. Natl. Cancer. Inst., 62:1415–1421 (1979).

    Google Scholar 

  5. H. J. Evans and M. L. O’Riordan, in: “Handbook of Mutagen Test Procedures,” B. J. Kilbey, M. Legator, and C. Ramel, eds., pp. 261–274, Elsevier/North Holland, Amsterdam, New York, Oxford (1977).

    Google Scholar 

  6. A. Brogger, in: “Genetic Damage in Man Caused by Environmental Agents,” K. Berg, ed., pp. 87–99, Academic Press, New York (1979).

    Google Scholar 

  7. P. Perry and H. J. Evans, Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange, Nature, 258: 121–125 (1975).

    Article  ADS  Google Scholar 

  8. D. G. Stetka and S. Wolff, Sister chromatid exchange as an assay for genetic damage induced by mutagen-carcinogens. I. In vivo test for compounds requiring metabolic activation, Mutat. Res., 41:333–341 (1976).

    Article  Google Scholar 

  9. S. Wolff, Sister chromatid exchanges, Ann. Rev. Genet., 11:183–201 (1977).

    Article  Google Scholar 

  10. S. Latt, J. W. Allen, W. E. Rogers, and L. A. Luerglus, in: “Handbook of Mutagen Test Procedures,” B. J. Kilbey, M. Legator, W. Nichols, and C. Ramel, eds., pp. 275–291, Elsevier/North Holland, Amsterdam, New York, Oxford (1977).

    Google Scholar 

  11. R. DeMars, in: “Banbury Report 2: Mammalian Cell Mutagenesis: The Maturation of Test Systems,” A. W. Hsie, J. P. O’Neill, and V. K. McElheny, eds., pp. 329–340, Cold Spring Harbor Laboratory (1979).

    Google Scholar 

  12. K. C. Atwood, The presence of A2 erythrocytes in A1 blood, Proc. Natl. Acad. Sci. (USA), 44:1054–1057 (1958).

    Article  ADS  Google Scholar 

  13. K. C. Atwood and S. L. Scheinberg, Somatic variation in human erythrocyte antigens, J. Cell. Comp. Physiol., 52:97–123 (1958).

    Article  Google Scholar 

  14. H. E. Sutton, in: “Mutagenic Effects of Environmental Contaminants,” H. E. Sutton and M. I. Harris, eds., pp. 121–128, Academic Press, New York (1972).

    Google Scholar 

  15. H. E. Sutton, in: “Birth Defects: Proceedings of the Fourth International Conference,” A. G. Motulsky and W. Lenz, eds., pp. 212–214 (1974).

    Google Scholar 

  16. G. Stamatoyannapoulos, W. G. Wood, T. N. Papayannopoulou, and P. E. Nute, An atypical form of hereditary persistance of fetal hemoglobin in blacks and its association with sickle cell trait, Blood, 46:683–692 (1975).

    Google Scholar 

  17. W. G. Wood, G. Stamatoyannapoulos, G. Lim, and P. E. Nute, F-cells in the adult: Normal values and levels in individuals with hereditary and acquired elevations of HbF, Blood, 46:671–682 (1975).

    Google Scholar 

  18. K. C. Atwood and F. J. Petter, Erythrocyte automosaicism in some persons of known genotype, Science, 134:2100–2102 (1961).

    Article  ADS  Google Scholar 

  19. T. H. Papayannopoulou, M. Brice, G. Stamatoyannopoulos, Hemoglobin F synthesis in vitro: Evidence for control at the level of primitive erythroid stem cells, Proc. Natl. Acad. Sci. (USA), 74:2923–2927 (1977).

    Article  ADS  Google Scholar 

  20. T. H. Papayannopoulou, P. E. Nute, G. Stamatoyannopoulos, and T. G. McGuire, Hemoglobin ontogenesis: Test of the gene exclusion hypothesis, Science, 197:1215–1216 (1977).

    Article  ADS  Google Scholar 

  21. T. H. Papayannopoulou, T. C. McGuire, G. Lim, E. Garzel, P. E. Nute, and G. Stamatoyannopoulos, Identification of hemoglobin S in red cells and normoblasts using fluorescent anti-Hb antibodies, Br. J. Haematol., 34: 25–31 (1976).

    Article  Google Scholar 

  22. T. H. Papayannopoulou, G. Lim, T. C. McGuire, V. Ahern, P. E. Nute, and G. Stamatoyannopoulos, Use of specific fluorescent antibody for the identification of a hemoglobin C in erythocytes, Am. J. Hematol., 2:95–112 (1977).

    Article  Google Scholar 

  23. G. Stamatoyannopoulos, P. E. Nute, T. H. Papayarinopoulou, T. G. McGuire, G. Lim, H. F. Bunn, and D. Rucknagel, Development of a somatic mutation screening system using Hb mutants. IV. Successful detection of red cells containing the human frame-shift mutants Hb Wayne and Hb Cranston using monospecific fluorescent antibodies, Am. J. Hum. Genet., 32:484–496 (1980).

    Google Scholar 

  24. G. H. Strauss and R. J. Albertini, Enumeration of 6-thioguanine resistant peripheral blood lymphocytes in man as a potential test for somatic cell mutation arising in vivo, Mutat. Res., 61:353–379 (1979).

    Article  Google Scholar 

  25. R. J. Albertini, Drug resistant lymphocytes in man as indicators of somatic cell mutation, Teratog. Carcinog. Mutagen., 1: 25–48 (1980).

    Article  Google Scholar 

  26. M. Lesch and W. L. Nyhan, A familial disorder of uric acid metabolism and central nervous system function, Am. J. Med., 36:561–570 (1964).

    Article  Google Scholar 

  27. J. E. Seegmiller, F. M. Rosenbloom, and W. N. Kelley, Enzyme defect associated with a sex linked human neurological disorder and excessive purine synthesis, Science, 155:1682–1684 (1967).

    Article  ADS  Google Scholar 

  28. W. N. Kelley, Enzymology and Biochemistry, A. HG-PRT deficiency in the Lesch-Nyhan syndrome and gout, Fed. Pros., 27:1047–1052 (1968).

    Google Scholar 

  29. C. T. Caskey and G. D. Kruh, The HPRT locus, Cell, 6:1-(1979).

    Article  Google Scholar 

  30. G. B. Elion, Biochemistry and pharmacology of purine analogs, Fed. Proc., 26:898–904 (1967).

    Google Scholar 

  31. G. B. Elion and G. H. Hitchings, in: “Advances in Chemotherapy”,: A. Goldin, F. Hawkin, and R. J. Schnitzer, eds., Vol. 2, pp. 91–177, Academic Press, New York (1965).

    Google Scholar 

  32. R. J. Albertini and R. DeMars, Mosaicism of peripheral blood lymphocyte populations in females heterozygous for the Lesch-Nyhan mutation, Biochem. Genet., 11:397–411 (1974).

    Article  Google Scholar 

  33. R. J. Albertini and R. DeMars, Detection and quantification of x-ray induced mutation in cultured human fibroblasts, Mutat. Res., 18:199–224 (1973).

    Article  Google Scholar 

  34. L. Jacobs and R. DeMars, in: “Handbook of Mutagenicity Test Procedures,” B. J. Kilbey, M. Legator, W. Nichols, and C. Ramel, eds., pp. 193–220, Elsevier/North Holland, Amsterdam, New York, Oxford (1977).

    Google Scholar 

  35. R. J. Albertini, D. L. Sylwester, and E. F. Allen, in: “Mutagenicity: New Horizons in Genetic Toxicology,” J. A. Heddle, ed., pp. 305–336, Academic Press, New York (1982).

    Google Scholar 

  36. G. H. Strauss, R. J. Albertini, P. Krusinski, and R. D. Baughman, 6-thioguanine resistant peripheral blood lymphocytes in humans following psoralen long-wave light therapy, J. Invest. Dermatol., 73:211–216 (1979).

    Article  Google Scholar 

  37. G. H. Strauss, E. F. Allen, and R. J. Albertini, An enumerative assay purine analogue resistant lymphocytes in women heterozygous for the Lesch-Nyhan mutation, Biochem. Genet., 18:529–547 (1980).

    Article  Google Scholar 

  38. J. Dancis, P. H. Berman, V. Jansen, and M. E. Balis, Absence of mosaicism in the lymphocyte in x-linked congenital hyperuricemia, Life Sci., 7:587–591 (1968).

    Article  Google Scholar 

  39. J. A. McDonald and W. N. Kelley, Lesch-Nyhan syndrome: Absence of the mutant enzyme in erythrocytes of a heterozygote for both normal and mutant hypoxanthine-guanine phosphoribosyltransferase, Biochem. Genet., 6:21–26 (1972).

    Google Scholar 

  40. W. L. Nyhan, B. Bakay, J. D. Connor, J. F. Marks, and D. K. Keele, Hemizygous expression of heterozygotes for the Lesch-Nyhan syndrome, Proc. Natl. Acad. Sci. (USA), 65:214–218 (1970).

    Article  ADS  Google Scholar 

  41. R. J. Albertini, E. F. Allen, A. S. Quinn, and M. R. Albertini, in: “Population and Biological Aspects of Human Mutation: Birth Defects Institute Symposium XI,” E. B. Hook and I. H. Porter, eds., pp. 235–263, Academic Press, New York (1981).

    Google Scholar 

  42. A. L. Maizel, S. R. Mehta, S. Häuft, D. Franzini, L. B. Lachman, and R. J. Ford, Human T-lymphocyte/monocyte interaction in response to lectin: Kinetics of entry into S-phase, J. Immunol., 127:1058–1064 (1981).

    Google Scholar 

  43. C. Allison, T. Hovi, R. W. E. Watts, and A. B. D. Webster, in: “Purine and Pyrimidine Metabolism,” K. Elliot and D. W. Fitzsimmons, eds., pp. 207–224, Elsevier/North Holland/Excerpta Medica, Amsterdam (1977).

    Google Scholar 

  44. T. Hovi, A. C. Allison, K. O. Raivio, and A. Vaheri, in: “Purine and Pyrimidine Metabolism,” K. Elliot and D. W. Fitzsimmons, eds., pp. 207–224, Elsevier/North Holland/Excerpta Medica, Amsterdam (1977).

    Google Scholar 

  45. R. J. Albertini, W. R. Borcherding, Cloning in vitro of human 6-thioguanine resistant peripheral blood lymphocytes arising in vivo, Environ. Mutagenis. (abstract) in press (1982).

    Google Scholar 

  46. R. J. Albertini, K. Castle, and W. R. Borcherding, T-Cell Cloning to Detect the Mutant 6-Thioguanine-Resistant Lymphocytes Present in Human Peripheral Blood, Proc. Natl. Acad. Sci. (USA) 79:6617–6621 (1982).

    Article  ADS  Google Scholar 

  47. R. J. Albertini, D. L. Sylwester, E. F. Allen, and B. D. Dannenberg, in: “Carcinogens and Mutagens in the Environment,” H. F. Stich, ed., Vol. I, CRC Press, Boca Ratan, Florida (in press) (1982).

    Google Scholar 

  48. R. J. Albertini, D. L. Sylwester, B. D. Dannenberg, and E. F. Allen, in: “Genetic Toxicology, An Agricultural Perspective,” R. Flech, ed., Plenum Press, New York (1982) (in press).

    Google Scholar 

  49. E. H. Y. Chu and S. S. Powell, in: “Advances in Human Genetics,” Harris and Hirshhorn, eds., Vol. 7, pp.189–258, Plenum Press, New York (1976).

    Google Scholar 

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Authors and Affiliations

  1. University of Vermont College of Medicine, Given Medical Building E305, 05405, Burlington, Vermont, USA

    Richard J. Albertini

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  1. National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA

    Frederick J. de Serres  & William Sheridan  & 

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© 1983 Plenum Press, New York

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Albertini, R.J. (1983). Human Mutagenicity Monitoring: Studies with 6-Thioguanine Resistant Lymphocytes. In: de Serres, F.J., Sheridan, W. (eds) Utilization of Mammalian Specific Locus Studies in Hazard Evaluation and Estimation of Genetic Risk. Environmental Science Research, vol 28. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3739-3_2

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  • DOI: https://doi.org/10.1007/978-1-4613-3739-3_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-3741-6

  • Online ISBN: 978-1-4613-3739-3

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