Advertisement

Detection of Enzyme Activity Variants in Mice

  • Daniel A. Casciano
  • Jack B. Bishop
  • Robert R. Delongchamp
  • Ritchie J. Feuers
Part of the Environmental Science Research book series (ESRH, volume 28)

Abstract

The measurement and assessment of mutagenic risk to man partially depends upon further development of test methods which detect a broad spectrum of induced lesions in experimental animals [1, 2]. In addition to the phenotypic specific locus test and test systems which detect transmitted chromosomal damage, several promising biochemical test methods have been proposed [2–4]. However, these latter approaches measure qualitative characteristics of proteins to detect variants having alterations in enzyme electrophoretic mobility. Approximately two-thirds of all possible amino acid substitutions result in no apparent change in net charge but may result in an unstable protein. Additionally, some deletions, chain terminations, etc., may lead to inactive or absent proteins [5]. Modification of electrophoretic methods to detect these types of genetic alteration requires the laborious construction of specific genetic stocks.

Keywords

Dose Group Mating Pair Aberrant Activity Ethyl Methanesulfonate Mating Interval 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    L. R. Valcovic and H. V. Mailing, An approach to measuring germinal mutations in the mouse, Environ. Health Perspect., 6: 201–205 (1973).CrossRefGoogle Scholar
  2. 2.
    H. V. Mailing and L. R. Valcovic, New approaches to detecting gene mutations in mammals, in: “Advances in Modern Toxicology,” W. G. Flamm and M. A. Mehlman, eds., Vol. 5, pp. 149–171, Hemisphere, Washington, D.C. (1978).Google Scholar
  3. 3.
    J. Klose, Isoelectric focusing and electrophoresis combined as a method for defining new point mutations in the mouse, Genetics, 92:513–524 (1970).Google Scholar
  4. 4.
    K.R. Narayanana, Detection of biochemical mutants in mice, Arch. Toxicol., 39:61–73 (1979).Google Scholar
  5. 5.
    H. Mohrenweiser, Frequency of enzyme deficiency variants in erythrocytes of newborn infants, Proc. Natl. Acad. Sci. USA, 78:5046–5050 (1981).ADSCrossRefGoogle Scholar
  6. 6.
    R. J. Feuers, R. R. Delongchamp, D. A. Casciano, J. G. Burkhart, and H. W. Mohrenweiser, Assay for mouse tissue enzymes: levels of activity and statistical variation for 29 enzymes of liver or brain, Anal. Biochem., 101:895–903 (1980).CrossRefGoogle Scholar
  7. 7.
    R. J. Feuers, J. B. Bishop, R. R. Delongchamp, and D. A. Casciano, Development of a new biochemical mutation test in mice based upon measurement of enzyme activities: 1. Theoretical concepts and basic procedure, Mutat. Res., 95:263–271 (1982).CrossRefGoogle Scholar
  8. 8.
    J. B. Bishop and R. J. Feuers, Development of a new biochemical mutation test in mice based upon measurement of enzyme activities: 2. Test results with ethyl methanesulfonate (EMS), Mutat. Res., 95:273–285 (1982).CrossRefGoogle Scholar
  9. 9.
    T. O. Tiffany, D. D. Chilcote, and C. A. Burtis, Evaluation of kinetic enzyme parameters by use of a small computer interfaced “fast analyzer” — an addition to automated clinical enzymology, Clin. Chem., 19:908–918 (1973).Google Scholar
  10. 10.
    H. N. Kirkman, Enzyme defects, in: “Prog. Med. Genet.,” A. F. Steinburg, ed., Vol. 8, pp. 125–168, Grune and Stratton, New York (1972).Google Scholar
  11. 11.
    K. Paigen, Acid hydrolases as models of genetic control, Ann. Rev. Genet., 13:417–466 (1979).CrossRefGoogle Scholar
  12. 12.
    K. M. Plowman, Enzyme Kinetics, McGraw-Hill Book Company, Inc., New York (1972).Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Daniel A. Casciano
    • 1
  • Jack B. Bishop
    • 1
  • Robert R. Delongchamp
    • 1
  • Ritchie J. Feuers
    • 1
  1. 1.Department of Health and Human Services Food and Drug AdministrationNational Center for Toxicological ResearchJeffersonUSA

Personalised recommendations