Detection of Enzyme Activity Variants in Mice
The measurement and assessment of mutagenic risk to man partially depends upon further development of test methods which detect a broad spectrum of induced lesions in experimental animals [1, 2]. In addition to the phenotypic specific locus test and test systems which detect transmitted chromosomal damage, several promising biochemical test methods have been proposed [2–4]. However, these latter approaches measure qualitative characteristics of proteins to detect variants having alterations in enzyme electrophoretic mobility. Approximately two-thirds of all possible amino acid substitutions result in no apparent change in net charge but may result in an unstable protein. Additionally, some deletions, chain terminations, etc., may lead to inactive or absent proteins . Modification of electrophoretic methods to detect these types of genetic alteration requires the laborious construction of specific genetic stocks.
KeywordsCage Glutam Electrophoresis Germinal Pyruvate
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- 2.H. V. Mailing and L. R. Valcovic, New approaches to detecting gene mutations in mammals, in: “Advances in Modern Toxicology,” W. G. Flamm and M. A. Mehlman, eds., Vol. 5, pp. 149–171, Hemisphere, Washington, D.C. (1978).Google Scholar
- 3.J. Klose, Isoelectric focusing and electrophoresis combined as a method for defining new point mutations in the mouse, Genetics, 92:513–524 (1970).Google Scholar
- 4.K.R. Narayanana, Detection of biochemical mutants in mice, Arch. Toxicol., 39:61–73 (1979).Google Scholar
- 9.T. O. Tiffany, D. D. Chilcote, and C. A. Burtis, Evaluation of kinetic enzyme parameters by use of a small computer interfaced “fast analyzer” — an addition to automated clinical enzymology, Clin. Chem., 19:908–918 (1973).Google Scholar
- 10.H. N. Kirkman, Enzyme defects, in: “Prog. Med. Genet.,” A. F. Steinburg, ed., Vol. 8, pp. 125–168, Grune and Stratton, New York (1972).Google Scholar
- 12.K. M. Plowman, Enzyme Kinetics, McGraw-Hill Book Company, Inc., New York (1972).Google Scholar