Detection of Enzyme Activity Variants in Mice

  • Daniel A. Casciano
  • Jack B. Bishop
  • Robert R. Delongchamp
  • Ritchie J. Feuers
Part of the Environmental Science Research book series (ESRH, volume 28)


The measurement and assessment of mutagenic risk to man partially depends upon further development of test methods which detect a broad spectrum of induced lesions in experimental animals [1, 2]. In addition to the phenotypic specific locus test and test systems which detect transmitted chromosomal damage, several promising biochemical test methods have been proposed [2–4]. However, these latter approaches measure qualitative characteristics of proteins to detect variants having alterations in enzyme electrophoretic mobility. Approximately two-thirds of all possible amino acid substitutions result in no apparent change in net charge but may result in an unstable protein. Additionally, some deletions, chain terminations, etc., may lead to inactive or absent proteins [5]. Modification of electrophoretic methods to detect these types of genetic alteration requires the laborious construction of specific genetic stocks.


Dose Group Mating Pair Aberrant Activity Ethyl Methanesulfonate Mating Interval 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Daniel A. Casciano
    • 1
  • Jack B. Bishop
    • 1
  • Robert R. Delongchamp
    • 1
  • Ritchie J. Feuers
    • 1
  1. 1.Department of Health and Human Services Food and Drug AdministrationNational Center for Toxicological ResearchJeffersonUSA

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