Abstract
In our previous presentation in this Symposium series, we described the development of a coupled Salmonella mutagenesis/reverse-phase high performance liquid chromatography (HPLC) procedure for the subfractionation of mutagens from complex mixtures of drinking water organics. Using the 20-year-old drinking water residue mixture available to us at that time, we demonstrated the reproducibility of this system. A group of potent, microsomal-activation-dependent, strain TA100 mutagens was detected and partially purified. For one of the repeat HPLC subfractions, further purification by gas chromatography (GC) of multiple aliquots on an SE 30 column yielded 10-μg amounts of purified compound (Tabor and Loper, 1980; Loper and Tabor, 1981). This substance is the “something old” alluded to in our title. Part of the “something new” is that we have determined this substance to be a previously unrecognized mutagen; we will have more to say about it later (see Discussion).
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Loper, J.C., Tabor, M.W. (1983). Isolation of Mutagens from Drinking Water: Something Old, Something New. In: Waters, M.D., Sandhu, S.S., Lewtas, J., Claxton, L., Chernoff, N., Nesnow, S. (eds) Short-Term Bioassays in the Analysis of Complex Environmental Mixtures III. Environmental Science Research, vol 27. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3611-2_11
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