Bacillus Subtilis Rec-Assay With and Without Metabolic Activation: Improvements and Applications
This assay is one of the systems that is most inexpensive, rapid, and nonlaborious.
Bacillus subtilis, gram-positive, has a permeable cell membrane to most chemicals. A rec deficiency, rec45, has been selected among DNA repair-deficient markers because of wide spectra of sensitivities to many chemical mutagens.
Recent improvements using spores in place of vegetative cells enabled us to increase assay sensitivity (some 50-fold), to use S-9 (rat liver homogenate) for in vitro metabolic activation as well as to obtain reproducible quantitative data.
KeywordsBacillus Subtilis Inhibition Zone Sodium Nitrite Sorbic Acid Chemical Mutagen
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