Abstract
Cell strains can be used in a sensitive method for studying the process of carcinogenesis and are useful for identifying environmental agents that may be carcinogenic to humans. A wide range of organic and inorganic carcinogens are capable of inducing transformation. The inorganic metal salts include arsenic which is a known human carcinogen, although demonstration of its carcinogenicity in animals is lacking. The degree to which any agent-- a chemical, a virus, or irradiation-- transforms Syrian hamster cells and the extent to which one agent interacts with another from the same or a different category of carcinogens can be analyzed. Sequential treatment involving chemicals, viruses, and radiation are important, since combinations of various agents are probably responsible for an increased risk of cancer in human populations. The enhancement of chemical and viral transformation by chemicals is related to the length of chemical treatment and the interval between treatments. In chemical carcinogenesis, the mechanism of transformation enhancement by various agents is not associated with the rate of excision or post-replication repair in DNA, whereas the enhanced viral transformation by chemicals is related to additional sites for viral DNA attachment into cell DNA during repair of damaged DNA. Carcinogens affect a number of molecular processes of modify existing DNA and thus provide an explanation for carcinogenesis; in fact, in some systems some of these agents may also show mutagenic activity, and produce SCE and chromosomal aberrations. Quantitative approaches permit the study of dose-response relationships. The ability of the transformed cells to form tumors in animals makes mammalian cell models relevant to in vivo cancer studies. The in vitro results correlate well with results from life-time studies in mice and rats.
Keywords
- Transformation Frequency
- Sister Chromatid Exchange
- Syrian Hamster
- Chemical Carcinogen
- Cloning Efficiency
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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DiPaolo, J.A. (1983). Modulation of Induced Quantitative Mammalian Cell Transformation. In: Kolber, A.R., Wong, T.K., Grant, L.D., DeWoskin, R.S., Hughes, T.J. (eds) In Vitro Toxicity Testing of Environmental Agents. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3566-5_15
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