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Lung Cells Grown on Cellulose Membrane Filters as an In Vitro Model of the Respiratory Epithelium

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Genotoxic Effects of Airborne Agents

Part of the book series: Environmental Science Research ((ESRH,volume 25))

Abstract

Airborne agents enter the body for the most part by first contacting some part of the respiratory epithelium. Particulates and gases may cross the epithelium by solution in the cell membranes or pinocytosis. Insoluble particles may be engulfed and removed by macrophages and mucociliary clearance mechanisms, but macrophages engorged with particulate materials may remain in the lung for prolonged times and serve as a source for continued exposure of other cells to potential toxins.

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References

  1. C. G. Plopper, D. L. Dungworth and W. S. Tyler, Morphometric evaluation of pulmonary lesions in rats exposed to ozone. Am. J. Pathol. 71: 395–408 (1973).

    Google Scholar 

  2. M. J. Evans, R. J. Stephens, L. J. Cabral and G. Freeman, Cell renewal in the lungs of rats exposed to low levels of NO2. Arch. Environ. Health 27: 180–188 (1972).

    Google Scholar 

  3. C. E. Cross, A. J. de Lucia, A. K. Reddy, M. Z. Hussain, C. K. Chow and M. G. Mustafa, Ozone interactions with tissue: Biochemical approaches. Am. J. Med. 60: 929–935 (1976).

    Google Scholar 

  4. B. D. Goldstein, C. Lodi, C. Collinson and O. J. Balchum, Ozone and lipid peroxidation. Arch. Environ. Health 18: 631–635 (1969).

    Google Scholar 

  5. Y. Berwald and L. Sachs, In vitro transformation of normal cells to tumor cells by carcinogenic hydrocarbons. J. Natl. Cancer Inst. 35:641–661 (1965).

    Google Scholar 

  6. J. DiPaolo and P. Donovan, Properties of Syrian hamster cells transformed in the presence of carcinogenic hydrocarbons. Exptl. Cell Res. 48: 361–377 (1967).

    Article  Google Scholar 

  7. R. J. Mason, M. C. Williams, R. D. Greenleaf and J. A. Clements, Isolation and properties of Type II alveolar cells. Am. Rev. Resp. Pis. 115: 1015–1026 (1977).

    Google Scholar 

  8. Y. Kikkawa and K. Yoneda, The Type II epithelial cell of the lung. Lab. Invest. 30: 76–84 (1974).

    Google Scholar 

  9. T. R. Devereaux and J. R. Fouts, The nonciliated cell bronchiolar epithelial (Clara) cell of the rabbit lung: Isolation and identification. Fed. Proc. 1092 (1980).

    Google Scholar 

  10. D. M. Pace, J. R. Thompson, B. T. Aftonomos and H. G. O. Hoick, The effects of NO2 and salts of NO2 upon established cell lines. Can. J. Biochem. Physiol. 39: 1247–1255 (1961).

    Article  Google Scholar 

  11. R. H. Fetner, Ozone-induced chromosome breakage in human cell cultures. Nature 194: 793–794 (1962).

    Article  ADS  Google Scholar 

  12. C. Voisin, C. Aerts, E. Jakubczak and A. B. Tonnel, La culture cellulaire en phase gazeuse. Bull. Europ. phisopath. Resp. 13: 69–82 (1977).

    Google Scholar 

  13. G. M. Alink, J. C. M. van der Hoeven, F. M. H. Debets, W. S. M. ven de Ven and J. H. Koeman, A new exposure model for in vitro testing of effects of gaseous pollutants on mammalian cells by means of gas diffusion through plastic films. Chemosphere 2: 63–73 (1979).

    Google Scholar 

  14. R. B. Painter, Rapid test to detect agents that damage human DNA. Nature 265: 650–651 (1977).

    Article  ADS  Google Scholar 

  15. P. Perry and S. Wolff, A new giemsa method for the differential Nature 251: 156–158 (1974).

    Article  ADS  Google Scholar 

  16. G. S. Samuelsen, R. E. Rasmussen, B. K. Nair and T. T. Crocker, Novel culture and exposure system for measurement of effects of airborne pollutants on mammalian cells. Env. Sci. Techno1. 12: 426–430 (1978).

    Article  Google Scholar 

  17. H. J. Phillips, Dye exclusion tests for cell viability, in: Tissue Culture Methods and Applications, P. F. Kruse, Jr., and M. K. Patterson, Jr., Eds., Academic Press, New York, 406–408 (1973).

    Google Scholar 

  18. P. C. Gooch, D. A. Creasia and J. G. Brewen, The cytogenetic effects of ozone: Inhalation and in vitro exposures. Environ. Res. 12: 188–195 (1976).

    Article  Google Scholar 

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© 1982 Plenum Press, New York

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Rasmussen, R.E., Crocker, T.T. (1982). Lung Cells Grown on Cellulose Membrane Filters as an In Vitro Model of the Respiratory Epithelium. In: Tice, R.R., Costa, D.L., Schaich, K.M. (eds) Genotoxic Effects of Airborne Agents. Environmental Science Research, vol 25. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3455-2_9

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  • DOI: https://doi.org/10.1007/978-1-4613-3455-2_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-3457-6

  • Online ISBN: 978-1-4613-3455-2

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