Abstract
In order to prevent in humans cancers as a result of exposure to environmental carcinogens, suitable methodologies and bioassays must be developed to detect all classes of carcinogenic agents. Quantitative cell culture systems provide procedures for detecting potential carcinogens in a relatively short period of time compared with the years required to complete in vivo tests. Mammalian cells in culture are particularly appropriate because they represent an extension of the in vivo experimental models, eliminate host factors, afford a means to control test chemical levels, and can be conducted in a controlled environment. Quantitative linear dose-response relationships have been demonstrated for cell transformation using fibroblasts from hamster embryo cells (1–4). The transformation event data are consistent with a one-hit phenomenon indicating a direct cause and effect relationship (2,3,5).
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References
Y. Berwald and L. Sachs, In vitro transformation of normal cells to tumor cells by carcinogenic hydrocarbons, J. Natl. Cancer Inst. 35:641–661 (1965).
J. A. DiPaolo, P. J. Donovan, and R. L. Nelson, Quantitative studies of in vitro transformation by chemical carcinogens, J. Natl. Cancer Inst. 42: 867–874 (1969).
J. A. DiPaolo, P. J. Donovan, and R. L. Nelson, Quantitative studies of in vitro transformation of hamster cells by polycyclic hydrocarbons: Factors influencing the number of cells transformed, Nature New Biol. 236: 240–241 (1971).
T. Kuroki and H. Sato, Transformation and neoplastic development in vitro of hamster embryonic cells by 4-nitroquinolino-l- oxide and its derivatives, J. Natl. Cancer Inst. 41: 53–71 (1968).
T. T. Chen and C. Heidelberger, Quantitative studies on the malignant transformation of mouse prostate cells by carcinogenic hydrocarbons in vitro, Intern. J. Cancer 4: 166–178 (1969).
B. C. Casto, Enhancement of adenovirus transformation by treatment of hamster cells with ultraviolet irradiation, DNA base analogs, and dibenz(a,h)anthracene, Cancer Res. 33: 402–407 (1973).
B. C. Casto, Enhancement of viral oncogenesis by chemical carcinogens, in: “Chemical Carcinogenesis. The Biochemistry of Disease”, P. O. P. Ts’o and J. A. DiPaolo, eds., Vol. 4, Marcel Dekker, Inc., N.Y., 607–618 (1974).
B. C. Casto, W. J. Pieczynski, and J. A. DiPaolo, Enhancement 87 of adenovirus transformation by t cells with diverse chemical carcinogens, Cancer Res. 34: 72–78 (1974).
B. C. Casto, W. J. Pieczynski, and J. A. DiPaolo, Enhancement of adenovirus transformation by pretreatment of hamster cells with carcinogenic polycyclic hydrocarbons, Cancer Res. 33: 819–824 (1978).
B. C. Casto, Detection of chemical carcinogens and mutagens in hamster cells by enhancement of adenovirus transformation in: “Advances in Modern Environmental Toxicology”, Vol. 1, N. M. Mishra, V. Dunkel, and M. Mehlman, eds., Senate Press, Princeton, N.J., 241–271 (1981).
B. C. Casto, Detection of chemical carcinogens and mutagens in hamster cells by enhancement of adenovirus transformation in: “Advances in Modern Environmental Toxicology”, Vol. 1, N. M. Mishra, V. Dunkel, and M. Mehlman, eds., Senate Press, Princeton, N.J., 241–271 (1981).
J. McCann, E. Choi, E. Yamasaki, and B. N. Ames, Detection of carcinogens as mutagens in the Salmonella/microsome test: Assay of 300 chemicals, Proc. Natl. Acad. Sci. (U.S.A.) 72: 5135–5139 (1975).
J. McCann and B. N. Ames, Detection of carcinogens as mutagens in the Salmonella/microsome test: Assay of 300 chemicals, Part II, Proc. Natl. Acad. Sci. (U.S.A.) 73: 950–954 (1976).
B. C. Casto, G. G. Hatch, S. L. Huang, J. L. Huisingh, S. Nesnow, and M. D. Waters, Mutagenic and carcinogenic potency of extracts of diesel and related environmental emissions: In Vitro mutagenesis and oncogenic transformation, in: “Health Effects of Diesel Engine Emissions”, Proceedings of an International Symposium, Vol. 2, USEPA-600/9-80-057a 843–860 (1980).
B. C. Casto, Adenovirus transformation of hamster embryo cells, J. Virol. 2: 376–383 (1968).
A. E. Freeman, P. H. Black, R. Wolford, and R. J. Huebner, Adenovirus type 12-rat embryo transformation system, J. Virol. 1: 362–367 (1967).
B. C. Casto, W. J. Peiczynski, N. Janosko, and J. A. DiPaolo, Significance of treatment interval and DNA repair in the enhancement of viral transformation by chemical carcinogens and mutagens, Chem.-Biol. Interactions 13: 105–125 (1976).
G. J. Todaro and H. Green, Enhancement by thymidine analogs of susceptibility of cells to transformation by SV40, Virology 24: 393–400 (1964).
R. J. Lorenz, Zur statistik des plaque–Testes, Archiv. Gesamte Virus forschung 12: 108–137 (1962).
IARC Mongraphs 11, 247, Lyon, France (1976).
E. Sawicki, Atmospheric genotoxicants — What numbers do we collect?, in: “Application of Short-term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures”, M. D. Waters, S. Nesnow, J. L. Huisingh, S. S. Sandhu, and L. Claxton, eds., Environmental Science Research, Vol. 15, Plenum Press, N.Y., 171–194 (1978).
T. Page, R. H. Harris, and S. S. Epstein, Drinking water and cancer mortality in Louisiana, Science 193: 55–57 (1976).
D. F. Krahn, Utilization of the CHO/HGPRT System: metabolic activation and method for testing gases, in: “Banbury Report 2, Mammalian Cell Mutagenesis: The Maturation of Test Systems”, W. H. Abraham, J. P. O’Neill, and V. R. McElheny, eds., Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 249–276 (1979).
V. F. Simmon, K. Kauhanen, and R. G. Tardiff, Mutagenic activity of chemicals identified in drinking water, in: “Progress in Genetic Toxicology”, D. Scott, B. A. Bridges, and F. H. Sobels, eds., Elsevier/North Holland Biomedical Press, Amsterdam, 249–258 (1977).
G. S. Samuelson, R. E. Rasmussen, B. K. Nair, and T. T. Crocker, Pollutants on mammalian cells, Environ. Sci. and Tech. 12: 426–430 (1978).
P. J. Price, C. M. Hassett, and J. I. Mansfield, Transforming activities of trichloroethylene and proposed industrial alternatives, In Vitro 14: 290–293 (1978).
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© 1982 Plenum Press, New York
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Hatch, G.G., Mamay, P.D., Ayer, M.L., Casto, B.C., Nesnow, S. (1982). Methods for Detecting Gaseous and Volatile Carcinogens Using Cell Transformation Assays. In: Tice, R.R., Costa, D.L., Schaich, K.M. (eds) Genotoxic Effects of Airborne Agents. Environmental Science Research, vol 25. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3455-2_7
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DOI: https://doi.org/10.1007/978-1-4613-3455-2_7
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