Skip to main content
SpringerLink
Log in
Book cover

Handbook of Psychopharmacology pp 75–93Cite as

Radioreceptor Assays for Neurotransmitters and Drugs

  • Chapter

  • 89 Accesses

Abstract

Scientific progress is inexorably linked to the development of new methodologies. In the biological sciences, the past century has witnessed a progression from gross observation and classification to detailed studies of anatomical structure and biochemical processes at a molecular level. This shift in emphasis was made possible by the development of more sensitive and specific analytical techniques.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abramson, F. P., 1974, Femtomole level of analysis of biogenic amines and amino acids using functional group mass spectrometry, Anal. Biochem 51: 482–499.

    Article  Google Scholar 

  • Berson, S., Yalow, R., Bauman, A., Rothschild, M., and Newerly, K., 1956, Insulin-I131 metabolism in human subjects: Demonstration of insulin-binding globulin in the circulation of insulin-treated subjects, J. Clin. Invest 35: 170–190.

    Article  PubMed  CAS  Google Scholar 

  • Braestrup, C. Nielsen, M., Skovbjerg, H., and Gredal, O., 1981, β-Carboline-3-carboxylates and benzodiazepine receptors, in: GABA and Benzodiazepine Receptors (E. Costa, G. Di Chiara, and G. Gessa, eds.), pp. 147–155, Raven Press, New York.

    Google Scholar 

  • Burnett, G. B., Prange, A. J., Wilson, I. C., Jollitt, L., Creese, I., and Snyder, S. H., 1980, Adverse effects of anticholinergic antiparkinsonian drugs in tardive dyskinesia, Neuropsychobiology 6: 109–120.

    Article  PubMed  CAS  Google Scholar 

  • Burt, D. R., 1978, Criteria for receptor identification, in: Neurotransmitter Receptor Binding ( H. I. Yamamura, S.J. Enna, and M.J. Kuhar, eds.), pp. 41–55, Raven Press, New York.

    Google Scholar 

  • Calil, H. M., Avery, D. H., Hollister, L., Creese, I., and Snyder, S. H., 1979, Serum levels of neuroleptics measured by dopamine radioreceptor assay and some clinical observations, Psychiat. Res 1: 34–44.

    Article  Google Scholar 

  • Chang, R., and Snyder, S. H., 1978, Benzodiazepine receptors: Labeling in intact animals with 3H-flunitrazepam, Eur. J. Pharmacol 48: 213–218.

    Article  PubMed  CAS  Google Scholar 

  • Cheng, Y. C., and Prusoff, W. H., 1973, Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 percent inhibition (I50) of the enzymatic reaction, Biochem. Pharmacol 22: 3099–3108.

    Article  PubMed  CAS  Google Scholar 

  • Costa, E., Koslow, S. H., and Le Fevre, H. F., 1975, Mass fragmentography: a tool for studying transmitter function at synaptic level, in: Handbook of Psychopharmacology, Vol. 1 ( L. L. Iversen, S. D. Iversen, and S. H. Snyder, eds.), pp. 1–41, Plenum Press, New York.

    Google Scholar 

  • Creese, I., and Snyder, S. H., 1977, A simple and sensitive radioreceptor assay for antischizophrenic drugs in blood, Nature 270: 180–182.

    Article  PubMed  CAS  Google Scholar 

  • Creese, I., Manian, A., Prosser, T. D., and Snyder, S. H., 1978, 3H-Haloperidol binding to dopamine receptors in rat corpus striatum: Influence of chlorpromazine metabolites and derivatives, Eur. J. Pharmacol 47: 291–296.

    Google Scholar 

  • Enna, S. J., 1978, Radioreceptor assay techniques for neurotransmitters and drugs, in: Neurotransmitter Receptor Binding ( H. I. Yamamura, S.J. Enna, and M.J. Kuhar, eds.), pp. 127–139, Raven Press, New York.

    Google Scholar 

  • Enna, S. J., 1980a, Radioreceptor assays, in: Physico-Chemical Methodologies in Psychiatric Research ( I. Hanin and S. H. Koslow, eds.), pp. 83–101, Raven Press, New York.

    Google Scholar 

  • Enna, S. J., 19806, Radioreceptor assay: a simple, sensitive, and specific analytical tool, in: Receptor Binding Techniques (D. Bylund, ed.), pp. 257–271, Society for Neuroscience, Washington, D.C.

    Google Scholar 

  • Enna, S. J., and Kendall, D. A., 1981, Interaction of antidepressants with brain neurotrans-mitter receptors, J. Clin. Psychopharmacol 1: (Suppl.) 12–16.

    Article  CAS  Google Scholar 

  • Enna, S. J., and Snyder, S. H., 1975, Properties of γ-aminobutyric acid (GABA) receptor binding in rat brain synaptic membrane fractions, Brain Res. 100: 81–97.

    Article  PubMed  CAS  Google Scholar 

  • Enna, S. J., and Snyder, S. H., 1976, A simple, sensitive, and specific radioreceptor assay for endogenous GABA in brain tissue, J. Neurochem 26: 221–224.

    Article  PubMed  CAS  Google Scholar 

  • Enna, S. J., and Snyder, S. H., 1977, Influences of ions, enzymes, and detergents on γ-aminobutyric acid receptor binding in synaptic membranes of rat brain, Mol. Pharmacol 13: 442–453.

    PubMed  CAS  Google Scholar 

  • Enna, S. J., Stern, L., Wastek, G., and Yamamura, H. I., 1977«, Cerebrospinal fluid GABA variations in neurological disorders, Arch. Neurol 34: 683–685.

    Google Scholar 

  • Enna, S. J., Wood, J. H., and Snyder, S. H., 1977b, γ-Aminobutyric acid (GABA) in human cerebrospinal fluid: radioreceptor assay, J. Neurochem 28: 1121–1124.

    Google Scholar 

  • Enna, S. J., Ziegler, M. G., Lake, C. R., Wood, J. H., Brooks, B. R., and Butler, I. J., 1980, Cerebrospinal fluid γ-aminobutyric acid, in: Neurobiology of Cerebrospinal Fluid, Vol. 1 ( J. Wood, ed.), pp. 189–196, Plenum Press, New York.

    Google Scholar 

  • Ferkany, J. W., Smith, L. A., Seifert, W. E., Caprioli, R. M., and Enna, S. J., 1978, Measurement of gamma-aminobutyric acid (GABA) in blood, Life Sci. 22: 2121–2128.

    Article  PubMed  CAS  Google Scholar 

  • Fields, J., Reisine, T. D., and Yamamura, H. I., 1977, Biochemical demonstration of dopaminergic receptors in rat and human brain using 3H-spiroperidol, Brain Res. 136: 578–584.

    Article  PubMed  CAS  Google Scholar 

  • Freedberg, K. A., Innis, R. B., Creese, I., and Snyder, S. H., 1979, Antischizophrenic drugs: differential plasma protein binding and therapeutic activity, Life Sci. 24: 2467–2474.

    Article  PubMed  CAS  Google Scholar 

  • Gilman, A. G., 1970, A protein binding assay for adenosine 3,5-cyclic monophosphate, Proc. Natl. Acad. Sci. U.S.A 67: 305–312.

    Article  PubMed  CAS  Google Scholar 

  • Hollenberg, M. D., and Cuatrecasas, P., 1975, Biochemical identification of membrane receptors: principles and techniques, in: Handbook of Psychopharmacology, Vol. 1 ( L. L. Iversen, S. D. Iversen, and S. H. Snyder, eds.), pp. 129–177, Plenum Press, New York.

    Google Scholar 

  • Innis, R. B., Bylund, D. B., and Snyder, S. H., 1978, A simple, sensitive, and specific radioreceptor assay for β-adrenergic antagonist drugs, Life Sci. 23: 2031–2038.

    Article  PubMed  CAS  Google Scholar 

  • Korenman, S. G., 1970, Relation between estrogen inhibitory activity and binding to cytosol of rabbit and human uterus, Endocrinology 87: 1119–1123.

    Article  CAS  Google Scholar 

  • Lefkowitz, R., Roth, J., and Pastan, I., 1970, Radioreceptor assay of adrenocorticotropic hormone: new approach to assay of polypeptide hormones in plasma, Science 170: 633–635.

    Article  PubMed  CAS  Google Scholar 

  • Lloyd, K. G., and Hornykiewicz, O., 1978, Effect of chronic neuroleptic or L-dopa administration on GABA levels in rat substantia nigra, Life Sci. 21: 1489–1496.

    Article  Google Scholar 

  • Maggi, A., U’Prichard, D. C., and Enna, S. J., 1980, Differential effects of antidepressant treatment on brain monoaminergic receptors, Eur. J. Pharmacol 61: 91–98.

    Article  PubMed  CAS  Google Scholar 

  • Mohler, H., Polc, P., Cumin, R., Pieri, L., and Kettler, R., 1979, Nicotinamide, a brain constituent with benzodiazepine-like actions, Nature 278: 563–565.

    Article  PubMed  CAS  Google Scholar 

  • Nahorski, S. R., Batta, M. I., and Barnett, D. B., 1978, Measurement of β-adrenoceptor antagonists in biological fluids using a radioreceptor assay, Eur. J. Pharmacol 52: 393–396.

    Article  PubMed  CAS  Google Scholar 

  • Neethling, A. C., McCarthy, B., and Taljaard, J. J., 1980, γ-Aminobutyric acid in CSF, Lancet, Jan. 26, 211.

    Google Scholar 

  • Peroutka, S. J., and Snyder, S. H., 1979, Multiple serotonin receptors: differential binding of 3H-5-hydroxytryptamine, 3H-lysergic acid diethylamide, and 3H-spiroperidol, Mol. Pharmacol 16: 687–699.

    PubMed  CAS  Google Scholar 

  • Rodbard, D., and Lewald, J. E., 1970, Computer analysis of radioligand assay and radioimmunoassay data, Acta Endocrinol (Suppl) 147: 79–103.

    CAS  Google Scholar 

  • Saavedra, J., Brownstein, M., and Axelrod, J., 1973, A specific and sensitive enzymatic- isotope microassay for serotonin in tissue, J. Pharmacol Exp. Ther 186: 508–515.

    PubMed  CAS  Google Scholar 

  • Scatchard, G., 1949, The attractions of proteins for small molecules and ions, Ann. N.Y. Acad. Sci 51: 660–672.

    Article  CAS  Google Scholar 

  • Shibuya, J., Bowie, D., and Pert, C., 1977, Opiate receptor ligand in cerebrospinal fluid: a simple radioreceptor assay requiring no preliminary purification, Proc. Soc. Neurosci 3: 460.

    Google Scholar 

  • Shore, P. A., and Olin, J. S., 1958, Identification and chemical assay of norepinephrine in brain and other tissues, J. Pharmacol Exp. Ther 122: 295–300.

    PubMed  CAS  Google Scholar 

  • Simantov, R., Childers, S., and Snyder, S. H., 1977, Opioid peptides: differentiation by radioimmunoassay and radioreceptor assay, Brain Res. 135: 358–367.

    Article  PubMed  CAS  Google Scholar 

  • Skolnick, P., Marangos, P. J., Goodwin, F. K., Edwards, M., and Paul, S., 1978, Identification of inosine and hypoxanthine as endogenous inhibitors of 3H-diazepam binding in the central nervous system, Life Sci. 23: 1473–1480.

    Article  PubMed  CAS  Google Scholar 

  • Snyder, S. H., Axelrod, J., and Zweig, M., 1965, A sensitive and specific fluorescence assay for tissue serotonin, Biochem. Pharmacol 14: 831–835.

    Article  PubMed  CAS  Google Scholar 

  • Snyder, S. H., Baldessarini, R., and Axelrod, J., 1966, A sensitive and specific enzymatic isotopic assay for tissue histamine, J. Pharmacol Exp. Ther 153: 544–549.

    PubMed  CAS  Google Scholar 

  • Snyder, S. H., and Yamamura, H. I., 1977, Antidepressants and the muscarinic acetylcholine receptor, Arch. Gen. Psychiat 34: 236–239.

    PubMed  CAS  Google Scholar 

  • Speth, R. C., Wastek, G. J., Johnson, P. C., and Yamamura, H. I., 1978, Benzodiazepine binding in human brain: characterization using 3H-flunitrazepam, Life Sci. 22: 859–866.

    Article  PubMed  CAS  Google Scholar 

  • Tune, L. E., Creese, I., Coyle, J. T., Pearlson, G., and Snyder. S. H., 1980a, Low neuroleptic serum levels in patients receiving fluphenazine decanoate, Am. J. Psychiat 137: 80–82.

    PubMed  CAS  Google Scholar 

  • Tune, L. E., Creese, I., De Paulo, J. R., Slavney, P. R., Coyle, J. T., and Snyder, S. H., 19806, Clinical state and serum neuroleptic levels measured by radioreceptor assay in schizophrenia, Am. J. Psychiat 137: 187–190.

    Google Scholar 

  • Valdes, F., and Orrego, F., 1978, Electrically induced, calcium-dependent release of endogenous GABA from rat brain cortex slices, Brain Res. 141: 357–363.

    Article  PubMed  CAS  Google Scholar 

  • Von Euler, U. S., 1947, A specific sympathomimetic ergone in adrenergic nerve fibers (sympathin) and its relations to adrenaline and noradrenaline, Acta Physiol Scand. 12: 73–97.

    Article  Google Scholar 

  • Wood, J. H., Glaeser, B. S., Enna, S. J., and Hare, T. A., 1978, Verification and quantification of GABA in human cerebrospinal fluid, J. Neurochem 30: 291–293.

    Article  PubMed  CAS  Google Scholar 

  • Yamamura, H. I., Enna, S. J., and Kuhar, M.J. (eds.), 1978, Neurotransmitter Receptor Binding, Raven Press, New York.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology, University of Texas Medical School at Houston, Houston, Texas, 77025, USA

    S. J. Enna

  2. Department of Neurobiology and Anatomy, University of Texas Medical School at Houston, Houston, Texas, 77025, USA

    S. J. Enna

Authors
  1. S. J. Enna
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Pharmacology, University of Cambridge, UK

    Leslie L. Iversen  & Susan D. Iversen  & 

  2. Department of Neuroscience, Pharmacology and Psychiatry, The Johns Hopkins University School of Medicine, USA

    Solomon H. Snyder

Rights and permissions

Reprints and permissions

Copyright information

© 1982 Plenum Press, New York

About this chapter

Cite this chapter

Enna, S.J. (1982). Radioreceptor Assays for Neurotransmitters and Drugs. In: Iversen, L.L., Iversen, S.D., Snyder, S.H. (eds) Handbook of Psychopharmacology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3452-1_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-3452-1_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-3454-5

  • Online ISBN: 978-1-4613-3452-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation