Abstract
Spectrophotometry continues to be very important in analytical chemistry and is the single most important detection system in the clinical laboratory. Even recent clinical laboratory advances in high-pressure liquid chromatography and enzyme-linked immunochemistry depend on spectrophotometric measurements. Although measurements made at a single wavelength are most common, multiple-wavelength analyses are growing in importance and add to the capabilities of the modern laboratory. Multiple wavelength spectrophotometry has been used to measure multiple components in a complex mixture, to correct for interferences, and to increase sensitivity. Examples of these applications will be discussed individually with special reference to their relevance in clinical chemistry. These general principles will serve as an introduction to the three following contributions which describe commercial applications of multiple-wavelength spectrophotometry in the clinical chemistry laboratory. This chapter is not intended to be an exhaustive review, but to provide the reader with general insight into clinical chemistry applications and a bibliography to guide further study. Two recent reviews(1,2) are suggested for additional background.
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References
T. J. Porro, Double-wavelength spectroscopy, Anal. Chem., 44, 93A-103A (1972).
K. L. Ratzlaff and D. F. Natusch, Theoretical assessment of precision in dual wavelength spectrophotometric measurement, Anal. Chem., 49, 2170–2176 (1977).
D. A. Skoog and D. M. West, Fundamentals of Analytical Chemistry, p. 663, Holt, Rinehart, Winston, New York (1963).
D. B. Siano and D. E. Metzler, Band shapes of the electronic spectra of complex molecules, J. Chem. Phys., 51, 1856–1861 (1969).
D. E. Metzler, C. Harris, I. Yang, D. Siano, and J. A. Thomson, Band-shape analysis and display of fine structure in protein spectra: A new approach to perturbation spectroscopy, Biochem. Biophys. Res. Commun., 46, 1588–1597 (1972).
D. E. Metzler, C. M. Harris, R. J. Johnson, D. B. Siano, and J. A. Thomson, Spectra of 3-hydroxypyridines. Band-shape analysis and evaluation of tautomeric equilibria, Biochemistry, 12, 5377–5392 (1973).
C. M. Harris, R. J. Johnson, and D. E. Metzler, Band-shape analysis and resolution of electronic spectra of pyridoxal phosphate and other 3-hydroxypyridine-4-aldehydes, Biochim. Biophys. Acta, 421, 181–194 (1976).
M. L. Fonda and R. J. Johnson, Computer analysis of spectra of enzyme-substrate and enzyme-inhibitor complexes involving aspartate aminotransferase, J. Biol. Chem., 245, 2709–2716 (1970).
W. M. Allen, A simple method for analyzing complicated absorption curves, of use in the colorimetric determination of urinary steroids, J. Clin. Endocrinol., 10, 71–83 (1950).
S. B. Jensen and R. W. A. Oliver, A simplified general proof of the Allen correction equation and some comments concerning its applicability to the colorimetric analysis of oestriol, Clin. Chim. Acta, 44, 443–448 (1973).
E. Allen and W. Rieman III, Determining only one compound in a mixture, Anal. Chem., 25, 1325–1331 (1953).
B. Chance, Rapid and sensitive spectrophotometry. III. A double beam apparatus, Rev. Sci. Instrum., 22, 634–638 (1951).
B. Chance, Spectrophotometry of intracellular respiratory pigments, Science, 120, 767–775 (1954).
S. Shibata, M. Furukawa, and K. Goto, Dual-wavelength spectrophotometry, Anal. Chim. Acta, 46, 271–279 (1969).
R. L. Sellers, G. W. Lowy, and R. W. Kane, A versatile UV-VIS spectrophotometer, Am. Lab. (March), 61–68 (1973).
S. M. Gerchakov, Dual wavelength spectrophotometry. Determination of 1, 2, 4-benzene-tri-carboxylic acid and benzenepentacarboxylic acid, Spectros. Lett., 4, 403–409 (1971).
B. Chance and N. Graham, A rapid scanning dual wavelength spectrophotometer, Rev. Sci. Instrum., 42, 941–945 (1971).
R. Szentrimay and T. Kuwana, Double wavelength spectrophotometry with a rapid scanning instrument, Anal. Chem., 49, 1348–1352 (1977).
J. C. Cowles, Theory of dual-wavelength spectrophotometry for turbid samples, J. Opt. Soc. Am., 55, 690–693 (1965).
R. Rikmenspoel, The sensitivity and accuracy of dual-wavelength spectrophotometers, Appl. Opt., 3, 351–355, (1964).
R. W. Hendler, Limitations on the use of and interpretation of data from the Aminco-Chance dual-wavelength split-beam recording spectrophotometer and related instruments, Anal. Biochem., 94, 450–464 (1979).
W. Liley, Liquor amnii analysis in the management of the pregnancy complicated by rhesus sensitization. Am. J. Obstet. Gynecol., 82, 1359–1370 (1961).
W. Liley, Errors in the assessment of hemolytic disease from amniotic fluid, Am. J. Obstet. Gynecol., 86, 485–494 (1963).
R. W. Burnett, Instrumental and procedural sources of error in determination of bile pigments in amniotic fluid, Clin. Chem., 18, 150–154 (1972).
J. J. Pisano, Assay of catecholamine metabolites based on vanillin formation, in: The Thyroid and Biogenic Amines (J. E. Rail and I. J. Kopin, eds.), ch. 7, pp. 474–488, North-Holland, Amsterdam (1972).
S. H. Jackson, A direct-reading bilirubinometer incorporating hemolysis and turbidity correction, Clin. Chem., 11, 1051–1057 (1965).
S. H. Jackson and A. H. Hernandez, A new “bilirubinometer” and its use in estimating total and conjugated bilirubin in serum, Clin. Chem., 16, 462–465 (1970).
H. Levkoff, M. C. Westphal, and J. F. Finklea, Evaluation of a direct reading spectrophotometer for neonatal bilirubinometry, Am. J. Clin. Pathol., 54, 562–565 (1970).
M. Michaëlsson, Evaluation of a method for determination of bilirubin in serum using direct spectrophotometry, Scand. J. Clin. Lab. Invest., 30, 387–390 (1972).
AO Bilirubinometer Model 10200 Reference Manual, Scientific Instrument Division, American Optical Corporation, Buffalo, New York (1971).
T. A. Blumenfeld, C. F. Steinbrink, H. S. Cheskin, J. D. George, and T. P. Vogl, Manual micromethods for bilirubin determination in sera of adults and children and investigation of reasons for observed differences, Am. J. Clin. Pathol., 69, 388–397 (1978).
N. W. Tietz and E. A. Fiereck, The spectrophotometric measurement of carboxyhemoglobin, Ann. Clin. Lab. Sci., 3, 36–42 (1973).
Instructions IL182 Co-Oximeter, Instrumentation Laboratory, Inc., Lexington, Massachusetts (1968).
Instructions IL282 Co-Oximeter, Instrumentation Laboratory, Inc., Lexington, Massachusetts (1980).
A. Ramieri, Jr., P. Jatlow, and D. Seligson, New method for rapid determination of carboxyhemoglobin by use of double-wavelength spectrophotometry, Clin. Chem., 20, 278–281 (1974).
E. W. Catalano, G. F. Johnson, and H. M. Soloman, Measurement of erythrocyte glucose-6-phosphate dehydrogenase activity with a centrifugal analyzer, Clin. Chem., 21, 134–138 (1975).
T. E. Cook, R. E. Santini, and H. L. Pardue, Design and evaluation of a vidicon based derivative spectrophotometer, Anal. Chem., 49, 871–877 (1977).
T. C. O’Haver, Potential clinical applications of derivative and wavelength-modulation spectrometry, Clin. Chem., 25, 1548–1553 (1979).
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© 1982 Plenum Press, New York
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Witte, D.L. (1982). Introduction to Multiple-Wavelength Spectrophotometric Measurements in Analytical and Clinical Chemistry. In: Hercules, D.M., Hieftje, G.M., Snyder, L.R., Evenson, M.A. (eds) Contemporary Topics in Analytical and Clinical Chemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3418-7_2
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DOI: https://doi.org/10.1007/978-1-4613-3418-7_2
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